CHVB-066

CHVB-066 (Serial dilutions; 96 h) potently inhibits CHIKV replication in Vero E6 cells with an EC₅₀ of 0.6 μM and has a CC₅₀ of 25 μM, with no activity against SFV^[1].
CHVB-066 (0.5-32 μM; 30 min) dose-dependently inhibits the combined methyltransferase and guanylyltransferase enzymatic activity of purified wild-type CHIKV nsP1 at concentrations ranging from 0.5 to 32 μM^[1].
CHVB-066 (0.16-5.00 μM; 48 h) induces a dose-dependent reduction in CHIKV 899 viral RNA and infectious progeny production in Vero cells, with a significant >4-log₁₀ reduction at 5.00 μM after 48 h^[2].
CHVB-066 (0-12.5 μM; 4 days) inhibits wild-type rCHIKV LS3 replication in Vero E6 cells with an EC₅₀ of 0.65 μM; resistance requires the combined nsP1^S454G and nsP1^W456R mutations, with maximum resistance (18.5-fold) achieved when combined with nsP2^M703T and nsP3^L494P mutations^[2].
CHVB-066 (0.5-1000 μM) potently inhibits the guanylyltransferase activity of purified VEEV nsP1 with an IC₅₀ of <0.5 μM^[2].
CHVB-066 (0.001-100 μM) inhibits the methyltransferase activity of purified VEEV nsP1 with an IC₅₀ of 1.9 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.