CHVB-066 

CHVB-066 is an alphavirus non-structural protein nsP1 inhibitor that exhibits varying degrees of inhibitory activity against nsP1 of CHIKV and VEEV. CHVB-066 inhibits MTase and GTase activities and disrupts the capping process of viral RNA, including inhibiting CHIKV replication, reducing progeny viruses, and preventing cytopathic effects. It shows varying degrees of inhibitory activity against nsP1 of CHIKV and VEEV but lacks anti-SFV replication activity, displays cross-resistance with MADTP series compounds, and requires nsP2/nsP3 mutations to develop complete drug resistance. This inhibitor with varying inhibitory activity against nsP1 of CHIKV and VEEV can be widely applied to relevant studies on chikungunya virus infection and chikungunya fever^[1][2][3][4].

CHVB-066 (Serial dilutions; 96 h) potently inhibits CHIKV replication in Vero E6 cells with an EC₅₀ of 0.6 μM and has a CC₅₀ of 25 μM, with no activity against SFV^[1].
CHVB-066 (0.5-32 μM; 30 min) dose-dependently inhibits the combined methyltransferase and guanylyltransferase enzymatic activity of purified wild-type CHIKV nsP1 at concentrations ranging from 0.5 to 32 μM^[1].
CHVB-066 (0.16-5.00 μM; 48 h) induces a dose-dependent reduction in CHIKV 899 viral RNA and infectious progeny production in Vero cells, with a significant >4-log₁₀ reduction at 5.00 μM after 48 h^[2].
CHVB-066 (0-12.5 μM; 4 days) inhibits wild-type rCHIKV LS3 replication in Vero E6 cells with an EC₅₀ of 0.65 μM; resistance requires the combined nsP1^S454G and nsP1^W456R mutations, with maximum resistance (18.5-fold) achieved when combined with nsP2^M703T and nsP3^L494P mutations^[2].
CHVB-066 (0.5-1000 μM) potently inhibits the guanylyltransferase activity of purified VEEV nsP1 with an IC₅₀ of <0.5 μM^[2].
CHVB-066 (0.001-100 μM) inhibits the methyltransferase activity of purified VEEV nsP1 with an IC₅₀ of 1.9 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

339.43

C₁₉H₂₅N₅O

1309199-94-7

O=C(N1CCN(CC1)C2=NC(NCC)=NC(C)=C2)C3=CC=C(C)C=C3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Besztercei B, et al. Three nitrogen atoms turn old kinase inhibitors into new targetable remedy[J]. bioRxiv, 2026: 2026.04. 11.717649.  [Content Brief]

  [2]. Abdelnabi R, Kovacikova K, Moesslacher J, et al. Novel class of chikungunya virus small molecule inhibitors that targets the viral capping machinery[J]. Antimicrobial Agents and Chemotherapy, 2020, 64(7): 10.1128/aac. 00649-20.  [Content Brief]

  [3]. Kovacikova K. Chikungunya virus nonstructural protein 1 as an antiviral target[J]. Antimicrob Agents Chemother, 2020, 64(12): e01788-20.

  [4]. Kovacikova K, van Hemert M J. Small-molecule inhibitors of chikungunya virus: mechanisms of action and antiviral drug resistance[J]. Antimicrobial agents and chemotherapy, 2020, 64(12): 10.1128/aac. 01788-20.  [Content Brief]