Compstatin TFA
Based on 3 publication(s) in Google Scholar
Compstatin TFA, a 13-residue cyclic peptide, is a potent inhibitor of the complement system C3 with species specificity. Compstatin TFA binds to baboon C3 and is resistant to proteolytic cleavage in baboon blood (similar to humans). Compstatin TFA inhibits only the activation of primates’ complement system. Compstatin TFA exhibits IC50 values of 63 μM and 12 μM for classical and alterative complement pathway, respectively.
For research use only. We do not sell to patients.
- Purity: 99.11%
- Formula: C66H99N23O17S2.xC2HF3O2
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Compstatin TFA
More
Customer Validation & Images
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RT-PCR
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Flow Cytometry
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IF
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IF
Biological Activity
Compstatin exhibits an in vitro half-life in human blood of about 2 hr[2].
In solution, compstatin forms a β-turn at residues Gln-5–Gly-8 with the disulfide bridge Cys-2–Cys12, residues Ile-1–Val-4, and Thr-13, forming a hydrophobic cluster[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Compstatin is stable in baboon plasma for more than 24 h[1].
Pig xenografts survival is significantly longer in the Compstatin perfused group than in the control group[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Juvenile baboons (P. Anubis) weighing 10.5-28.8 kg[1].
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Dosage:50, 25 mg/kg 60 min after heparin and 2 min before protamine.
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Administration:A bolus injection.
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Result:Completely inhibited complement activation induced by heparin–protamine complexes.
Chemical Information
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Appearance Solid
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Formula C66H99N23O17S2.xC2HF3O2
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Color White to off-white
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Sequence
Ile-Cys-Val-Val-Gln-Asp-Trp-Gly-His-His-Arg-Cys-Thr-NH2 (Disulfide bridge: Cys2-Cys12)
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Sequence Shortening
ICVVQDWGHHRCT-NH2 (Disulfide bridge: Cys2-Cys12)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Compstatin TFA purchased from MedChemExpress. Usage Cited in: J Mater Chem B. 2019, 7, 4207-4216.
The transfection characterized by inverted fluorescence microscope. GFP-3T3 cells and GFP-MCF-7 cells cultured with 10% FBS were transfected with the addition of C3a or Compstatin (25 μM; 4 h) under the influence of magnetic fields or not.
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Eur J Immunol
Differences in Phage Recognition and Immunogenicity Contribute to Divergent Human Immune Responses to Escherichia coli and Klebsiella pneumoniae Phages. [Abstract]2025 Mar;55(3):e202451543. PMID: 40071703
Compstatin TFA purchased from MedChemExpress. Usage Cited in: Eur J Immunol. 2025 Mar;55(3):e202451543. [Abstract]
Ec70 and Kp127 phage internalization following inhibition of complement using Compstatin (C3 inhibitor, 50 µM; 15 min) or heat inactivation. Intracellular phage was measured by flow cytometry (SYBR labeling), plaque‐forming units (viable phage), and qPCR (intracellular genomes) (n = 8).
Compstatin TFA purchased from MedChemExpress. Usage Cited in: Eur J Immunol. 2025 Mar;55(3):e202451543. [Abstract]
C3 internalization was quantified by flow cytometry in phage+ and phage‐monocytes following complement inactivation using Compstatin (50 µM; 15 min) and heat inactivation (n = 7).
Compstatin TFA purchased from MedChemExpress. Usage Cited in: Eur J Immunol. 2025 Mar;55(3):e202451543. [Abstract]
Immunofluorescent imaging of monocytes treated with SYBR‐labelled phages (green) ± C3 inhibitor Compstatin (50 µM). Internalized C3 was labelled in red and cell nucleus in blue. Detected phages and C3 marked with a white arrow, scale bar = 2 µm.
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Glycobiology
Sialidases derived from Gardnerella vaginalis and Prevotella timonensis remodel the sperm glycocalyx and impair sperm function. [Abstract]2025 Nov 6;35(11):cwaf067. PMID: 41165775
Solvent & Solubility
DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (Need ultrasonic)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (285 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Soulika AM, et al. Inhibition of heparin/protamine complex-induced complement activation by Compstatin in baboons. Clin Immunol. 2000 Sep;96(3):212-21. [Content Brief]
[2]. Fiane AE, et al. Compstatin, a peptide inhibitor of C3, prolongs survival of ex vivo perfused pig xenografts. Xenotransplantation. 1999 Feb;6(1):52-65. [Content Brief]
[3]. Bert J C Janssen, et al. Structure of compstatin in complex with complement component C3c reveals a new mechanism of complement inhibition. J Biol Chem. 2007 Oct 5;282(40):29241-7. [Content Brief]
[4]. A Sahu, et al. Inhibition of human complement by a C3-binding peptide isolated from a phage-displayed random peptide library. J Immunol. 1996 Jul 15;157(2):884-91. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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