Methylophiopogonone A
Based on 1 Customer Validation
Methylophiopogonone A is a homoisoflavonoid compound found in the tuberous roots of Ophiopogon japonicus. Methylophiopogonone A inhibits lipopolysaccharide-induced NO production in mouse microglia with an IC50 of 19.2 μM. Methylophiopogonone A blocks the proliferation of LX-2 hepatic stellate cells and downregulates TGF-β1-induced expressions of type I collagen and α-SMA. Methylophiopogonone A blocks RELA/EGFR-mediated hepatic stellate cell activation, inhibits the MAPK12 pathway to reduce extracellular matrix accumulation, and simultaneously suppresses inflammatory signal transduction. Methylophiopogonone A can be used in studies related to chronic hepatitis B-associated hepatic fibrosis and neuroinflammation.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 74805-90-6
- Formula: C19H16O6
- Molecular Weight:340.33
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Collagen I |
Methylophiopogonone A (compound 10) (24 h) potently inhibits lipopolysaccharide-induced nitric oxide production in mouse microglial BV-2 cells, with an IC50 value of 19.2 μM[1].
Methylophiopogonone A (3.13-100 μM; 24-72 h) inhibits the proliferation of immortalized human hepatic stellate LX-2 cells in a time- and dose-dependent manner[2].
Methylophiopogonone A (3.13-25 μM; 24 h) dose-dependently inhibits the expression of type Ⅰ collagen and α-smooth muscle actin (α-SMA) in immortalized human hepatic stellate LX-2 cells induced by TGF-β1[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:immortalized human hepatic stellate LX-2 cells
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Concentration:3.13, 6.25, 12.5, 25, 50, 100 μM
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Incubation Time:24 h; 48 h; 72 h
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Result:Inhibited LX-2 cell viability in a time- and dose-dependent manner.
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Cell Line:immortalized human hepatic stellate LX-2 cells
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Concentration:3.13, 6.25, 12.5, 25 μM (in the presence of 5 ng/mL TGF-β1)
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Incubation Time:24 h
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Result:Inhibited TGF-β1-induced expression of collagen I and α-SMA.
Reduced protein levels of collagen I in a dose-dependent manner.
Reduced protein levels of α-SMA in a dose-dependent manner.
Chemical Information
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CAS No. 74805-90-6
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Appearance Solid
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Molecular Weight 340.33
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Formula C19H16O6
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Color Off-white to light yellow
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SMILES
O=C1C(CC2=CC=C(OCO3)C3=C2)=COC4=C(C)C(O)=C(C)C(O)=C14
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (293.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 12.5 mg/mL (36.73 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Li N, et al. Anti-inflammatory homoisoflavonoids from the tuberous roots of Ophiopogon japonicus. Fitoterapia. 2012 Sep;83(6):1042-5. [Content Brief]
[2]. Li G, et al. Active Ingredients and Action Mechanisms of Yi Guan Jian Decoction in Chronic Hepatitis B Patients with Liver Fibrosis. Evidence-based complementary and alternative medicine : eCAM. 2019;2019:2408126. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9383 mL | 14.6916 mL | 29.3832 mL | 73.4581 mL |
| 5 mM | 0.5877 mL | 2.9383 mL | 5.8766 mL | 14.6916 mL | |
| 10 mM | 0.2938 mL | 1.4692 mL | 2.9383 mL | 7.3458 mL | |
| 15 mM | 0.1959 mL | 0.9794 mL | 1.9589 mL | 4.8972 mL | |
| 20 mM | 0.1469 mL | 0.7346 mL | 1.4692 mL | 3.6729 mL | |
| 25 mM | 0.1175 mL | 0.5877 mL | 1.1753 mL | 2.9383 mL | |
| 30 mM | 0.0979 mL | 0.4897 mL | 0.9794 mL | 2.4486 mL | |
| 40 mM | 0.0735 mL | 0.3673 mL | 0.7346 mL | 1.8365 mL | |
| 50 mM | 0.0588 mL | 0.2938 mL | 0.5877 mL | 1.4692 mL | |
| 60 mM | 0.0490 mL | 0.2449 mL | 0.4897 mL | 1.2243 mL | |
| 80 mM | 0.0367 mL | 0.1836 mL | 0.3673 mL | 0.9182 mL | |
| 100 mM | 0.0294 mL | 0.1469 mL | 0.2938 mL | 0.7346 mL |
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