2. Apoptosis
  3. Apoptosis
  4. (±)-Forbesione

(±)-Forbesione, a potential apoptosis inducer, is a racemate of Forbesione (HY-N7892). (±)-Forbesione inhibits proliferation of cancer cells. (±)-Forbesione can be used for cancer research.

For research use only. We do not sell to patients.

(±)-Forbesione

(±)-Forbesione Chemical Structure

CAS No. : 667914-50-3

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

(±)-Forbesione Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

(±)-Forbesione, a potential apoptosis inducer, is a racemate of Forbesione (HY-N7892). (±)-Forbesione inhibits proliferation of cancer cells. (±)-Forbesione can be used for cancer research[1].

In Vitro

(±)-Forbesione (range of concentrations; 24 h) exhibits low micromolar antiproliferative activity against HepG2, A549, and U251 cells, with IC50 values of 4.07 μM, 3.58 μM, and 8.57 μM, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(±)-Forbesione Related Antibodies

Cell Cycle Analysis[1]

Cell Line: Ham-1 cholangiocarcinoma cells
Concentration: 0.5 µM; 1 µM; 2 µM; 4 µM; 8 µM
Incubation Time: 24 h
Result: Induced concentration-dependent S-phase cell cycle arrest (P<0.01).
Reduced the proportion of cells in the G0/G1 (P<0.05) and G2/M (P<0.05) phases.
Increased the percentage of cells in the sub-G1 fraction (apoptotic cells) significantly in a concentration-dependent manner (P<0.01).

Apoptosis Analysis[1]

Cell Line: Ham-1 cholangiocarcinoma cells
Concentration: 2 µM; 4 µM; 8 µM; 16 µM
Incubation Time: 24 h
Result: Induced bright green, condensed, and fragmented chromatin, which are hallmarks of apoptosis, in treated cells, while untreated cells showed uniformly green-stained, normal nuclei.

Apoptosis Analysis[1]

Cell Line: Ham-1 cholangiocarcinoma cells
Concentration: 2 µM; 4 µM; 8 µM; 16 µM; 32 μM
Incubation Time: 6, 24, 48 h
Result: Increased the percentage of apoptotic cells in a concentration- and time-dependent manner.
Resulted in 50.75% (P<0.01), 55.57% (P<0.001), and 64.43% (P<0.001) apoptotic cells at 6, 24, and 48 h respectively when treated with 32 µM.

Western Blot Analysis[1]

Cell Line: Ham-1 cholangiocarcinoma cells
Concentration: 2 µM; 4 µM; 8 µM
Incubation Time: 24 h
Result: Significantly decreased protein expression of cyclin E (P<0.01), cyclin A (P<0.05), and cyclin-dependent kinase 2 (Cdk2) (P<0.01).
Significantly increased protein expression of p21 (P<0.05) and p27 (P<0.05) compared to control cells.\nIncreased protein expression of Fas (P<0.05), Fas-associated death domain (FADD) (P<0.05), and activated caspase-3 (P<0.05) in the death receptor pathway.
Decreased protein expression of procaspase-8 (P<0.05) and procaspase-3 (P<0.01) in the death receptor pathway.
Increased protein expression of B-cell lymphoma-2-like protein 4 (Bax) (P<0.05), activated caspase-9 (P<0.05), and activated caspase-3 (P<0.05) in the mitochondrial pathway.
Decreased protein expression of B-cell lymphoma-2 (Bcl-2) (P<0.05), procaspase-9 (P<0.05), and procaspase-3 (P<0.01) in the mitochondrial pathway.
Increased protein expression of activated caspase-12 (P<0.05), activated caspase-9 (P<0.05), and activated caspase-3 (P<0.05) in the endoplasmic reticulum pathway.
Decreased protein expression of procaspase-12 (P<0.05), procaspase-9 (P<0.05), and procaspase-3 (P<0.01) in the endoplasmic reticulum pathway.\nSignificantly decreased protein expression of NF-κB/p65 (P<0.01) compared to control cells.
Significantly increased protein expression of inhibitor of κB-α (IκB-α) (P<0.05) compared to control cells.\nSignificantly decreased protein expression of cytokeratin 19 (CK19) (P<0.01) compared to control cells.
Significantly decreased protein expression of proliferating cell nuclear antigen (PCNA) (P<0.05) compared to control cells.

Apoptosis Analysis[3]

Cell Line: human cholangiocarcinoma KKU-100 cells
Concentration: 0.25 µM; 0.5 µM; 1 µM; 2 μM; 1 μM (combined with 0.025 μM Doxorubicin)
Incubation Time: 48 h
Result: Increased apoptosis in KKU-100 cells in a dose-dependent manner, with the highest single-agent dose (2 μM) inducing ~32% apoptotic cells.
Induced ~75% apoptotic cells when combined with 0.025 μM Doxorubicin, which was significantly higher than either single agent alone.

Western Blot Analysis[3]

Cell Line: human cholangiocarcinoma KKU-100 cells
Concentration: 0.25 μM; 0.5 μM; 1 μM (single agent); 0.25 μM + 0.006 μM Doxorubicin (C1); 0.5 μM + 0.012 μM Doxorubicin (C2); 1 μM + 0.025 μM Doxorubicin (C3)
Incubation Time: 48 h
Result: Decreased Bcl-2 expression (0.27-fold at 1 μM) and increased Bax expression (2.06-fold at 1 μM) as a single agent, leading to a Bax/Bcl-2 ratio of 1.63; the C3 combination further decreased Bcl-2 to 0.10-fold and increased Bax to 3.24-fold, resulting in a Bax/Bcl-2 ratio of 12.00.
Decreased survivin (0.35-fold at 1 μM), procaspase-9 (0.70-fold at 1 μM), and procaspase-3 (0.76-fold at 1 μM) while increasing activated caspase-9 (42.00-fold at 1 μM) and activated caspase-3 (42.00-fold at 1 μM) as a single agent; the C3 combination enhanced these effects, reducing procaspase-9 and procaspase-3 to 0-fold and increasing activated caspase-9 and caspase-3 to 64.00-fold and 54.00-fold, respectively.
Increased IκB-α (1.15-fold at 1 μM) and decreased pIκB-α (0.14-fold at 1 μM) and NF-κB/p65 (0.12-fold at 1 μM) as a single agent; the C3 combination further increased IκB-α to 1.21-fold and decreased pIκB-α and NF-κB/p65 to 0.01-fold each.
Decreased MRP1 expression (0.71-fold at 1 μM) as a single agent, while the C3 combination reduced MRP1 to 0.34-fold, a significantly greater effect than single-agent treatment.
In Vivo
Animal Model: Syrian hamsters (male, 6-8 weeks old, intradermal injection of 2.5×105 Ham-1 cholangiocarcinoma cells)[1]
Dosage: 50 mg/kg
Administration: p.o.; daily; 4 weeks
Result: Reduced tumor volume (P<0.01) and mean tumor weight from 0.54 g (control) to 0.27 g (P<0.001).
Significantly decreased relative CK19 mRNA expression in tumor tissues (P<0.05).
Significantly increased relative Bax, Apaf-1, caspase-9, and caspase-3 mRNA expression in tumor tissues (P<0.05).
Significantly decreased expression of CK19 (P=0.036), PCNA (P=0.032), cyclin A (P=0.028), and Bcl-2 (P=0.039) in tumor tissues via immunohistochemical analysis.
Significantly increased expression of Bax (P=0.039), caspase-9 (P=0.028), and caspase-3 (P=0.039) in tumor tissues via immunohistochemical analysis.
Increased body weight (P<0.001), food intake (P<0.001), and water intake (P<0.05) in treated hamsters.
Caused no significant histopathological changes in liver, kidney, or stomach, and no changes in serum liver (ALT, ALP) or kidney (BUN, creatinine) function markers.
Molecular Weight

464.55

Formula

C28H32O6
CAS No.
SMILES

C/C(C)=C\CC1(O2)C(C(C3)C2(C)C)(OC4=C(C(O)=C5)C/C=C(C)\C)C(C(C4=C5O)=O)=CC3C1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

(±)-Forbesione Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(±)-Forbesione667914-50-3ApoptosisHepG2 cellsapoptosisU251 cellsA549 cellscancerInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
(±)-Forbesione
Cat. No.:
HY-N7892A
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.