Autophagy inducer 3
Based on 1 publication(s) in Google Scholar
Autophagy inducer 3 has autophagy induced activity. Autophagy inducer 3 possesses robust autophagic cell death in diverse cancer cells sparing normal counterpart. Autophagy inducer 3 induces lethal autophagy by formation of characteristic autophagic vacuoles, LC3 puncta formation, upregulation of signature autophagy markers like Beclin and Atg family proteins.
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- Pureza: 98.02%
- No. CAS: 2691054-63-2
- Fòrmula: C24H43NO2
- Peso molecular:377.60
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Autophagy inducer 3
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Actividad biológica
Autophagy[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCD-18Co | IC50 |
>10 μM
Compound: 26b
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Antiproliferative activity against human CCD-18Co cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
Antiproliferative activity against human CCD-18Co cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
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[PMID: 31926468] |
| COLO 205 | IC50 |
2.03 μM
Compound: 26b
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Antiproliferative activity against human COLO 205 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
Antiproliferative activity against human COLO 205 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
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[PMID: 31926468] |
| DLD-1 | IC50 |
4.46 μM
Compound: 26b
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Antiproliferative activity against human DLD-1 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
Antiproliferative activity against human DLD-1 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
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[PMID: 31926468] |
| HT-29 | IC50 |
4.15 μM
Compound: 26b
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Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
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[PMID: 31926468] |
| LoVo | IC50 |
3.33 μM
Compound: 26b
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Antiproliferative activity against human LoVo cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
Antiproliferative activity against human LoVo cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
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[PMID: 31926468] |
| SW48 | IC50 |
3.14 μM
Compound: 26b
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Antiproliferative activity against human SW48 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
Antiproliferative activity against human SW48 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
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[PMID: 31926468] |
| SW-620 | IC50 |
1.86 μM
Compound: 26b
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Antiproliferative activity against human SW-620 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
Antiproliferative activity against human SW-620 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay
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[PMID: 31926468] |
Autophagy inducer 3 (compound 26b) (10 μM; 48 hours) effectively inhibits cell growth in diverse breast, lung and colon cancer cell lines[1].
Autophagy inducer 3 (0-10 μM; 48 hours) exhibits potent antiproliferative activity in COLO-205, LOVO, HT-29, DLD-1, SW48 and SW-620 with IC50s of 2.03 μM, 3.33 μM, 4.15 μM, 4.46 μM, 3.14 μM, 1.86 μM, respectively; and shows low cytotoxicity in normal human colon fibroblast CCD-18Co with IC50 over 10 μM[1].
Autophagy inducer 3 (7.5 μM; 18 hours) promotes non apoptotic cell death in DLD-1 cells by cellular granularity through vacuole formation and increase Annexin-V positive cells as well as PARP cleavage; and robustly induces the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7, MDA-MB-231, DLD-1, HT-29, A549 and NCI-H358[1]
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Concentration:10 μM
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Incubation Time:48 hours
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Result:Effectively inhibited cell growth at 10 μM dose in diverse cancer cell lines with the inhibition rates of 89.28~97.66%.
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Cell Line:DLD-1[1]
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Concentration:7.5 μM
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Incubation Time:18 hours
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Result:Promoted intracytoplasmic vacuole accumulation in colon cancer (DLD-1) cells; and robustly induced the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7.
Chemical Information
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No. CAS 2691054-63-2
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Appearance Solid
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Peso molecular 377.60
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Fòrmula C24H43NO2
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Color White to off-white
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SMILES
CCCCCCCCCCCCCC[C@H](O)[C@@H](N)CC1=CC=C(C=C1)OC
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
Solvente y solubilidad
Ethanol : 100 mg/mL (264.83 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.62 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: 2.5 mg/mL (6.62 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureza y Documentación
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Ficha de datos (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.6483 mL | 13.2415 mL | 26.4831 mL | 66.2076 mL |
| 5 mM | 0.5297 mL | 2.6483 mL | 5.2966 mL | 13.2415 mL | |
| 10 mM | 0.2648 mL | 1.3242 mL | 2.6483 mL | 6.6208 mL | |
| 15 mM | 0.1766 mL | 0.8828 mL | 1.7655 mL | 4.4138 mL | |
| 20 mM | 0.1324 mL | 0.6621 mL | 1.3242 mL | 3.3104 mL | |
| 25 mM | 0.1059 mL | 0.5297 mL | 1.0593 mL | 2.6483 mL | |
| 30 mM | 0.0883 mL | 0.4414 mL | 0.8828 mL | 2.2069 mL | |
| 40 mM | 0.0662 mL | 0.3310 mL | 0.6621 mL | 1.6552 mL | |
| 50 mM | 0.0530 mL | 0.2648 mL | 0.5297 mL | 1.3242 mL | |
| 60 mM | 0.0441 mL | 0.2207 mL | 0.4414 mL | 1.1035 mL | |
| 80 mM | 0.0331 mL | 0.1655 mL | 0.3310 mL | 0.8276 mL | |
| 100 mM | 0.0265 mL | 0.1324 mL | 0.2648 mL | 0.6621 mL |
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