BODIPY−DOX
BODIPY-DOX is a conjugate composed of BODIPY and Doxorubicin (HY-15142A), as well as a pH-activated fluorescent probe for M1 macrophages and an apoptosis inducer. BODIPY-DOX undergoes pH-induced hydrazone bond cleavage in acidic M1 macrophage phagosomes, thereby releasing cytotoxic Doxorubicin (Dox) and inhibiting the function of pro-inflammatory M1 macrophages. BODIPY-DOX highly selectively inhibits the production of relevant pro-inflammatory cytokines by mouse and human monocyte-derived M1 macrophages, while exerting minimal effects on M2 or unactivated macrophages. Therefore, BODIPY-DOX enables simultaneous fluorescent tracing, differentiation and elimination of specific macrophage subsets, and exhibits the potential to regulate tissue regeneration in zebrafish models.
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- No. CAS: 2173116-67-9
- Fòrmula: C54H62BF2N7O12
- Peso molecular:1049.92
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
- Pureza y Documentación
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Help & FAQs
Actividad biológica
BODIPY-DOX (5-10 μM; overnight) induces dose-dependent cytotoxicity in LPS-polarized M1 RAW264.7 murine macrophages, but does not affect cell viability in nonactivated or IL-4-polarized M2 RAW264.7 murine macrophages[1].
BODIPY-DOX (10 μM; 20 h) activates to release fluorescent signal and induce apoptosis specifically in LPS-polarized M1 RAW264.7 murine macrophages, with minimal effects on nonactivated RAW264.7 murine macrophages[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LPS-polarized M1 RAW264.7 murine macrophages, IL-4-polarized M2 RAW264.7 murine macrophages, nonactivated RAW264.7 murine macrophages
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Concentration:5 μM, 10 μM
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Incubation Time:overnight
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Result:Reduced normalized cell viability of M1 macrophages to ~0.8.
Reduced normalized cell viability of M1 macrophages to ~0.65.
Did not reduce normalized cell viability in nonactivated or M2 macrophages at either concentration.
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Cell Line:LPS-polarized M1 RAW264.7 murine macrophages, nonactivated RAW264.7 murine macrophages
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Concentration:10 μM
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Incubation Time:20 h
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Result:Showed strong BODIPY fluorescence staining in LPS-polarized M1 macrophages.
Increased apoptotic index (Annexin V-positive cells) of LPS-polarized M1 macrophages to 3-fold higher than that of nonactivated macrophages.
Exhibited coincident strong BODIPY fluorescence in apoptotic M1 macrophages.
Showed low BODIPY fluorescence staining and a low apoptotic index in nonactivated macrophages.
Chemical Information
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No. CAS 2173116-67-9
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Peso molecular 1049.92
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Fòrmula C54H62BF2N7O12
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SMILES
[H][C@@]1(C[C@](/C(CO)=N\NC(CCC2=[N+]3C(C(C)=C2)=C(C4=C(C=C(N4[B-]3(F)F)C)C)C5=CC=C(C=C5)NC(CN(CC)CC)=O)=O)(CC6=C(C7=C(C(O)=C61)C(C8=C(C=CC=C8C7=O)OC)=O)O)O)O[C@H]9C[C@@H]([C@@H]([C@@H](O9)C)O)N
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BODIPY−DOX Related Classifications
HY-D3190 Related Classifications
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