hUP1-IN-2

hUP1-IN-2 (Compound 10a) at a concentration of 1 μM inhibits the catalytic activity of recombinant hUP1 by 80%^[1].
hUP1-IN-2 acts as a competitive inhibitor of recombinant hUP1 against Urd (K_i = 68 nM), and a non-competitive inhibitor against Pᵢ (K_i = 127 nM)^[1].
hUP1-IN-2 binds to free recombinant hUP1, the hUP1-P_i binary complex, and the hUP1-R1P binary complex via exothermic reactions, with the highest affinity for the hUP1-P_i complex (K_d = 151 nM)^[1].
hUP1-IN-2 exhibits negligible inhibitory activity against thymidine phosphorylase, with an IC₅₀ >100 μM^[1].
hUP1-IN-2 (1-100 μM; 72 h) exerts no significant in vitro cytotoxicity against HaCat, HT-29 or SW-620 cells when applied at concentrations ranging from 1 to 100 μM for 72 h^[1].
hUP1-IN-2 (30 μM; 72 h) does not alter the cytotoxicity of 5-FU in HaCat cells, reduces the cytotoxicity of 5-FU in HT-29 cells, and enhances the cytotoxicity of 5-FU in SW-620 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.