GW311616 hydrochloride
Based on 11 publication(s) in Google Scholar
GW-311616 is a potent, orally bioavailable, long duration and selective human neutrophil elastase (HNE) inhibitor with IC50 value of 22 nM and Ki value of 0.31 nM.
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- Pureza: 99.96%
- No. CAS: 197890-44-1
- Fòrmula: C19H32ClN3O4S
- Peso molecular:433.99
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) GW311616 hydrochloride
More- Adv Sci (Weinh). 2023 Aug;10(24):e2302613. [Abstract]
- J Invest Dermatol. 2020 Jul;140(7):1371-1378.e3. [Abstract]
- Life Sci. 2020 Feb 1:242:117229. [Abstract]
- EMBO Rep. 2023 Oct 9;24(10):e57032. [Abstract]
- Int Immunopharmacol. 2023 Jan:114:109537. [Abstract]
- Sci Rep. 2017 Nov 30;7(1):16628. [Abstract]
- Genes Immun. 2026 Jun 23. [Abstract]
- J Leukoc Biol. 2022 Dec;112(6):1427-1443. [Abstract]
- Cell Immunol. 2020 Jun:352:104101. [Abstract]
- BMC Immunol. 2025 Aug 30;26(1):63. [Abstract]
- Res Sq. 2025 Mar 30.
Actividad biológica
GW-311616 (150 μM; 48 hours) markedly suppresses NE activity in U937 and K562 cells lines[2].
GW311616A (20-320 μM; 48 hours; U937 cells) treatment inhibits proliferation and induces apoptosis in leukemia cells[2].
GW-311616 (150 μM; U937 cells) treatment can increase the protein expression levels of Bax and decrease the expression of Bcl-2[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U937 and K562 cells
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Concentration:150 μM
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Incubation Time:48 hours
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Result:Markedly suppressed NE activity.
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Cell Line:U937 cells
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Concentration:20 μM, 40 μM, 80 μM, 160 μM, 320 μM
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Incubation Time:48 hours
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Result:The rate of apoptosis was enhanced.
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Cell Line:U937 cells
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Concentration:150 μM
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Incubation Time:48 hours
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Result:Increased the protein expression levels of Bax and decreased the expression of Bcl-2.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Dogs (9-month-old)[3]
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Dosage:0.22 mg/kg, 0.66 mg/kg and 2 mg/kg (Pharmacokinetic study)
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Administration:Oral administration
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Result:At 0.22 mg/kg, greater than 50% inhibition of elastase is achieved 6 hours after dosing, with activity returning towards control values. Single oral dose of 2 mg/kg rapidly abolishes circulating enzyme activity, and greater than 90% inhibition is maintained for 4 days.
Chemical Information
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No. CAS 197890-44-1
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Appearance Solid
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Peso molecular 433.99
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Fòrmula C19H32ClN3O4S
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Color White to yellow
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SMILES
O=C1[C@@H](C(C)C)[C@@](N(C(/C=C/CN2CCCCC2)=O)CC3)([H])[C@]3([H])N1S(=O)(C)=O.[H]Cl
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Synonyms
GW311616A
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (11)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
FGF19-Induced Inflammatory CAF Promoted Neutrophil Extracellular Trap Formation in the Liver Metastasis of Colorectal Cancer. [Abstract]2023 Aug;10(24):e2302613. PMID: 37345586 -
J Invest Dermatol
Differences in Staining for Neutrophil Elastase and its Controlling Inhibitor SLPI Reveal Heterogeneity among Neutrophils in Psoriasis. [Abstract]2020 Jul;140(7):1371-1378.e3. PMID: 31945345 -
Life Sci
Neutrophil elastase-mediated proteolysis of the tumor suppressor p200 CUX1 promotes cell proliferation and inhibits cell differentiation in APL. [Abstract]2020 Feb 1:242:117229. PMID: 31887298 -
EMBO Rep
2023 Oct 9;24(10):e57032. PMID: 37650863 -
Int Immunopharmacol
Inhibition of neutrophil elastase prevents cigarette smoke exposure-induced formation of neutrophil extracellular traps and improves lung function in a mouse model of chronic obstructive pulmonary disease. [Abstract]2023 Jan:114:109537. PMID: 36495695 -
Sci Rep
Mineral particles stimulate innate immunity through neutrophil extracellular traps containing HMGB1. [Abstract]2017 Nov 30;7(1):16628. PMID: 29192209 -
Genes Immun
Neutrophil extracellular traps formation driven by novel ELANE mutations in cyclic neutropenia patients. [Abstract]2026 Jun 23. PMID: 42337056 -
J Leukoc Biol
Epigallocatechin-3-gallate reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis. [Abstract]2022 Dec;112(6):1427-1443. PMID: 35983712 -
Cell Immunol
Early recruited neutrophils promote asthmatic inflammation exacerbation by release of neutrophil elastase. [Abstract]2020 Jun:352:104101. PMID: 32278493 -
BMC Immunol
Neutrophil expression of CD41/CD61 complex contributes to their adhesiveness in both healthy individuals and heart failure patients. [Abstract]2025 Aug 30;26(1):63. PMID: 40885916 -
Solvente y solubilidad
DMSO : 100 mg/mL (230.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (230.42 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Pureza y Documentación
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Ficha de datos (284 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Ohbayashi H,et al. Neutrophil elastase inhibitors as treatment for COPD. Expert Opin Investig Drugs. 2002 Jul;11(7):965-80. [Content Brief]
[2]. Jiang KL, et al. Neutrophil elastase and its therapeutic effect on leukemia cells. Mol Med Rep. 2015 Sep;12(3):4165-4172. [Content Brief]
[3]. Macdonald SJ, et al. The discovery of a potent, intracellular, orally bioavailable, long duration inhibitor of human neutrophil elastase--GW311616A a development candidate. Bioorg Med Chem Lett. 2001 Apr 9;11(7):895-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.3042 mL | 11.5210 mL | 23.0420 mL | 57.6050 mL |
| 5 mM | 0.4608 mL | 2.3042 mL | 4.6084 mL | 11.5210 mL | |
| 10 mM | 0.2304 mL | 1.1521 mL | 2.3042 mL | 5.7605 mL | |
| 15 mM | 0.1536 mL | 0.7681 mL | 1.5361 mL | 3.8403 mL | |
| 20 mM | 0.1152 mL | 0.5761 mL | 1.1521 mL | 2.8803 mL | |
| 25 mM | 0.0922 mL | 0.4608 mL | 0.9217 mL | 2.3042 mL | |
| 30 mM | 0.0768 mL | 0.3840 mL | 0.7681 mL | 1.9202 mL | |
| 40 mM | 0.0576 mL | 0.2880 mL | 0.5761 mL | 1.4401 mL | |
| 50 mM | 0.0461 mL | 0.2304 mL | 0.4608 mL | 1.1521 mL | |
| 60 mM | 0.0384 mL | 0.1920 mL | 0.3840 mL | 0.9601 mL | |
| 80 mM | 0.0288 mL | 0.1440 mL | 0.2880 mL | 0.7201 mL | |
| 100 mM | 0.0230 mL | 0.1152 mL | 0.2304 mL | 0.5761 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
GW311616 hydrochloride Related Classifications
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