1. NF-κB
    Metabolic Enzyme/Protease
    Immunology/Inflammation
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  2. Reactive Oxygen Species
    Others
  3. HKOH-1r

HKOH-1r 

Cat. No.: HY-D1159 Purity: 97.26%
COA Handling Instructions

Reactive oxygen species (ROS) is a by-product of biological aerobic metabolism and is a general term for a class of oxygen-containing and active substances. In aerobic respiration, the vast majority of oxygen (O2) is reduced to water (H2O) in the respiratory chain. However, about 1% of O2 is incompletely reduced to produce superoxide ions (O2??). The superoxide ion can be converted into a series of ROS in the body, including hydrogen peroxide (H2O2), hypochlorous acid (HOCl), hydroxyl radicals (?OH) and peroxynitrite (ONOO?). ROS is continuously produced in the body and destroyed by antioxidant mechanisms, plays an important role in oxidative stress, cell division differentiation, immune activation, and aging, and is the cause of many major diseases (including tumors, cardiovascular diseases, and neurodegenerative diseases). Therefore, the specific identification and detection of ROS has been a research hotspot[1]. HKOH-1 series is a highly sensitive green fluorescent probe for the specific detection of ?OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively, with HKSOX-1m targeting labeling in mitochondria[2].

For research use only. We do not sell to patients.

HKOH-1r Chemical Structure

HKOH-1r Chemical Structure

CAS No. : 2138472-08-7

Size Price Stock Quantity
1 mg USD 300 In-stock
Estimated Time of Arrival: December 31
5 mg USD 900 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1550 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Reactive oxygen species (ROS) is a by-product of biological aerobic metabolism and is a general term for a class of oxygen-containing and active substances. In aerobic respiration, the vast majority of oxygen (O2) is reduced to water (H2O) in the respiratory chain. However, about 1% of O2 is incompletely reduced to produce superoxide ions (O2??). The superoxide ion can be converted into a series of ROS in the body, including hydrogen peroxide (H2O2), hypochlorous acid (HOCl), hydroxyl radicals (?OH) and peroxynitrite (ONOO?). ROS is continuously produced in the body and destroyed by antioxidant mechanisms, plays an important role in oxidative stress, cell division differentiation, immune activation, and aging, and is the cause of many major diseases (including tumors, cardiovascular diseases, and neurodegenerative diseases). Therefore, the specific identification and detection of ROS has been a research hotspot[1]. HKOH-1 series is a highly sensitive green fluorescent probe for the specific detection of ?OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively, with HKSOX-1m targeting labeling in mitochondria[2].

In Vitro

1.Preparation of HKOH-1 working solution 1.1Preparation of the stock solution Dissolve 1 mg HKOH-1 in 135 μL DMSO to obtain 10 mM of stock solution. Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles. 1.2Preparation of HKOH-1 working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution. Note: Please adjust the concentration of HKOH-1 working solution according to the actual situation. 2.Cell staining 2.1 Suspension cells(6-well plate) a.Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes. c.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. d.Wash twice with PBS, 5 minutes each time. e.Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

938.28

Formula

C33H21Cl2I2NO11

CAS No.
Emission (Em)
Excitation (Ex)
SMILES

O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OC5=CC(I)=C(O)C(I)=C5)C(Cl)=C4)OC6=CC(O)=C(Cl)C=C63.CC(N(CC(OC)=O)CC(OC)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 95 mg/mL (101.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0658 mL 5.3289 mL 10.6578 mL
5 mM 0.2132 mL 1.0658 mL 2.1316 mL
10 mM 0.1066 mL 0.5329 mL 1.0658 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 4.75 mg/mL (5.06 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 4.75 mg/mL (5.06 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 97.26%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HKOH-1r
Cat. No.:
HY-D1159
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