1. Immunology/Inflammation Others NF-κB Metabolic Enzyme/Protease
  2. Fluorescent Dye Reactive Oxygen Species
  3. HKOH-1r

HKOH-1r is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively.

For research use only. We do not sell to patients.

HKOH-1r Chemical Structure

HKOH-1r Chemical Structure

CAS No. : 2138472-08-7

Size Price Stock Quantity
1 mg USD 300 In-stock
5 mg USD 900 In-stock
10 mg USD 1550 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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1 Publications Citing Use of MCE HKOH-1r

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HKOH-1r is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively[1].

In Vitro

1. Preparation of HKOH-1r working solution
1.1 Preparation of the stock solution
Dissolve 1 mg HKOH-1r in 107 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HKOH-1r working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of? working solution.
Note: Please adjust the concentration of HKOH-1r working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

938.28

Formula

C33H21Cl2I2NO11

CAS No.
Appearance

Solid

Color

White to yellow

Emission (Em)

520

Excitation (Ex)

500

SMILES

O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OC5=CC(I)=C(O)C(I)=C5)C(Cl)=C4)OC6=CC(O)=C(Cl)C=C63.CC(N(CC(OC)=O)CC(OC)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 95 mg/mL (101.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0658 mL 5.3289 mL 10.6578 mL
5 mM 0.2132 mL 1.0658 mL 2.1316 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 4.75 mg/mL (5.06 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 4.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (47.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 4.75 mg/mL (5.06 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 4.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (47.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.43%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0658 mL 5.3289 mL 10.6578 mL 26.6445 mL
5 mM 0.2132 mL 1.0658 mL 2.1316 mL 5.3289 mL
10 mM 0.1066 mL 0.5329 mL 1.0658 mL 2.6644 mL
15 mM 0.0711 mL 0.3553 mL 0.7105 mL 1.7763 mL
20 mM 0.0533 mL 0.2664 mL 0.5329 mL 1.3322 mL
25 mM 0.0426 mL 0.2132 mL 0.4263 mL 1.0658 mL
30 mM 0.0355 mL 0.1776 mL 0.3553 mL 0.8881 mL
40 mM 0.0266 mL 0.1332 mL 0.2664 mL 0.6661 mL
50 mM 0.0213 mL 0.1066 mL 0.2132 mL 0.5329 mL
60 mM 0.0178 mL 0.0888 mL 0.1776 mL 0.4441 mL
80 mM 0.0133 mL 0.0666 mL 0.1332 mL 0.3331 mL
100 mM 0.0107 mL 0.0533 mL 0.1066 mL 0.2664 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
HKOH-1r
Cat. No.:
HY-D1159
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