2. Immunology/Inflammation Others NF-κB Metabolic Enzyme/Protease
  3. Fluorescent Dye Reactive Oxygen Species (ROS)
  4. HKOH-1r

HKOH-1r is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively.

For research use only. We do not sell to patients.

HKOH-1r

HKOH-1r Chemical Structure

CAS No. : 2138472-08-7

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Based on 8 publication(s) in Google Scholar

HKOH-1r Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

HKOH-1r is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively[1].

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of HKOH-1r working solution
1.1 Preparation of the stock solution
Dissolve 1 mg HKOH-1r in 107 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HKOH-1r working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of HKOH-1r working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HKOH-1r Related Antibodies
Molecular Weight

938.28

Formula

C33H21Cl2I2NO11
CAS No.
Appearance

Solid

Color

White to yellow

Emission (Em)

520
Excitation (Ex)

500
SMILES

O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OC5=CC(I)=C(O)C(I)=C5)C(Cl)=C4)OC6=CC(O)=C(Cl)C=C63.CC(N(CC(OC)=O)CC(OC)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 95 mg/mL (101.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0658 mL 5.3289 mL 10.6578 mL
5 mM 0.2132 mL 1.0658 mL 2.1316 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.43%

SDS (252 KB)

COA (256 KB) LCMS (96 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0658 mL 5.3289 mL 10.6578 mL 26.6445 mL
5 mM 0.2132 mL 1.0658 mL 2.1316 mL 5.3289 mL
10 mM 0.1066 mL 0.5329 mL 1.0658 mL 2.6644 mL
15 mM 0.0711 mL 0.3553 mL 0.7105 mL 1.7763 mL
20 mM 0.0533 mL 0.2664 mL 0.5329 mL 1.3322 mL
25 mM 0.0426 mL 0.2132 mL 0.4263 mL 1.0658 mL
30 mM 0.0355 mL 0.1776 mL 0.3553 mL 0.8881 mL
40 mM 0.0266 mL 0.1332 mL 0.2664 mL 0.6661 mL
50 mM 0.0213 mL 0.1066 mL 0.2132 mL 0.5329 mL
60 mM 0.0178 mL 0.0888 mL 0.1776 mL 0.4441 mL
80 mM 0.0133 mL 0.0666 mL 0.1332 mL 0.3331 mL
100 mM 0.0107 mL 0.0533 mL 0.1066 mL 0.2664 mL
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HKOH-1r Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HKOH-1r2138472-08-7Fluorescent DyeReactive Oxygen Species (ROS)endogenoushydroxylradicalsInhibitorinhibitorinhibit

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