Sodium orthovanadate
Based on 14 publication(s) in Google Scholar
Sodium orthovanadate is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue.
For research use only. We do not sell to patients.
- Purity: 99.40%
- CAS No.: 13721-39-6
- Formula: Na3VO4
- Molecular Weight:183.91
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Sodium orthovanadate
More- Science. 2024 Mar 22;383(6689):eadj4591. [Abstract]
- Nat Immunol. 2026 Jun 15. [Abstract]
- Cancer Res. 2025 Feb 17;85(4):692-703. [Abstract]
- ACS Nano. 2021 Sep 28;15(9):14360-14372. [Abstract]
- Nat Struct Mol Biol. 2025 Nov 12. [Abstract]
- Cell Death Dis. 2025 Jan 20;16(1):31. [Abstract]
- Cell Commun Signal. 2024 Jul 17;22(1):364. [Abstract]
- Dev Cell. 2025 May 5;60(9):1336-1347.e5. [Abstract]
- Cell Rep. 2023 Nov 13;42(11):113430. [Abstract]
- Int J Mol Sci. 2025 May 26;26(11):5100. [Abstract]
- Vet Microbiol. 2021 Nov:262:109241. [Abstract]
- Tissue Cell. 2023 Jun:82:102109. [Abstract]
- Res Sq. 2025 May 21.
- bioRxiv. 2024 Jul 12:2024.07.10.602941. [Abstract]
Customer Validation & Images
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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RT-PCR
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WB
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WB
Biological Activity
PPTPase[1].
In the presence of oxidizing agents vanadium ions exist as the hydrated monomer of Sodium orthovanadate (vanadate: HVO42- or H2VO4-) at micromolar concentations near neutral pH. Sodium orthovanadate (vanadate) also begins to polymerize at concentrations greater than 0.1 mM at neutral pH. The yellow-orange solutions of decavanadate can be converted to the colorless solutions of monomeric Sodium orthovanadate (vanadate) by dilution after a period of many hours. The process is hastened by boiling at pH 10, which encourages the kinetically sluggish depolymerization process[1].
Sodium orthovanadate could alter the phosphorylation status of ASK1 at serine 83 and threonine 845 induced by ischemia. Sodium orthovanadate could increase the tyrosine posphorylation of PTEN and further inhibit the activation of ASK1 via activating Akt during cerebral ischemia[2].
Sodium orthovanadate needs to be fully activated (depolymerized) to obtain maximum phosphatase inhibitory activity. The steps are as follows:
1. Dissolve sodium orthovanadate in water to prepare a 200mM solution. Weigh an appropriate amount of sodium orthovanadate powder and dissolve it in pure water.
2. Adjust pH to 10.0 with 1 M NaOH or 1 M HCl. At this time the solution appears yellow.
3. Heat and boil until the solution becomes colorless (boil for about 10 minutes), and all crystals must be fully dissolved.
4. Cool to room temperature.
5. Readjust the pH to 10.0 and repeat steps 3 and 4 until the solution remains colorless and the pH stabilizes at 10.0.
6. Divide the activated sodium orthovanadate solution into small portions (such as 1mL) and store at -20°C. The storage solution can be added directly to the cell or tissue lysis solution and diluted to a working solution such as 1 mM. Cell culture experiments need to be filtered and sterilized with a 0.2μm filter, and then diluted and added to the culture medium;
7. After taking out the aliquoted sample from -20°C and melting it, the solution needs to be heated to about 90~100°C, vortex and mix well to fully dissolve the crystals.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 13721-39-6
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Appearance Solid
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Molecular Weight 183.91
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Formula Na3VO4
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Color White to light yellow
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SMILES
[O].[Na].[V]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (14)
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Journal Impact Factor
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Most Recent
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Science
Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. [Abstract]2024 Mar 22;383(6689):eadj4591. PMID: 38513023 -
Nat Immunol
Pyoderma gangrenosum caused by the molecular uncoupling of OTULIN catalytic activity and LUBAC binding. [Abstract]2026 Jun 15. PMID: 42297973 -
Cancer Res
Adenosine Uptake through the Nucleoside Transporter ENT1 Suppresses Antitumor Immunity and T-cell Pyrimidine Synthesis. [Abstract]2025 Feb 17;85(4):692-703. PMID: 39652568 -
ACS Nano
Apoptotic Extracellular Vesicles Ameliorate Multiple Myeloma by Restoring Fas-Mediated Apoptosis. [Abstract]2021 Sep 28;15(9):14360-14372. PMID: 34506129 -
Nat Struct Mol Biol
2025 Nov 12. PMID: 41225050 -
Cell Death Dis
S1PR3-driven positive feedback loop sustains STAT3 activation and keratinocyte hyperproliferation in psoriasis. [Abstract]2025 Jan 20;16(1):31. PMID: 39833165 -
Cell Commun Signal
Targeting CCL2-CCR2 signaling pathway alleviates macrophage dysfunction in COPD via PI3K-AKT axis. [Abstract]2024 Jul 17;22(1):364. PMID: 39014433 -
Dev Cell
PCP-B peptides and CrRLK1L receptor kinases control pollination via pH gating of aquaporins in Arabidopsis. [Abstract]2025 May 5;60(9):1336-1347.e5. PMID: 39793583
Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Dev Cell. 2025 May 5;60(9):1336-1347.e5. [Abstract]
The hydration rates of WT pollen pollinated on the WT stigmas treated with the PM H+-ATPase inhibitor Na3VO4 (Sodium orthovanadate; 20-100 μM) and PS-2. n = 8 stigmas.
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Cell Rep
Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization. [Abstract]2023 Nov 13;42(11):113430. PMID: 37963021
Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Nov 13;42(11):113430. [Abstract]
Immunoblot assay of WT and Atg5 cKO pMs upon IL-4 treatment in the presence or absence of Sodium orthovanadate (Na3VO4, 50 μM) treatment.
Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Nov 13;42(11):113430. [Abstract]
Immunoblot assay of WT and Atg7 cKO pMs upon IL-4 treatment in the presence or absence of Sodium orthovanadate (Na3VO4, 50 μM) treatment. And quantitative real-time PCR analysis of IL-4 receptor genes. (n=3).
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Int J Mol Sci
BACE1 Inhibition Protects Against Type 2 Diabetes Mellitus by Restoring Insulin Receptor in Mice. [Abstract]2025 May 26;26(11):5100. PMID: 40507910
Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 26;26(11):5100. [Abstract]
Quantification of cytotoxic effects of Sodium orthovanadate (Na3VO4) on N2a cells ranging from 0–60 μM was examined by MTT assay.
Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 26;26(11):5100. [Abstract]
N2a cells were mock-treated or treated with Sodium orthovanadate (Na3VO4, 40 μM) for 1 h at 37 ℃ and then infected with CVS-11 at an MOI of 1 for 24 h. The cells were lysed to determine the RNA copy numbers of RABV N by RT-qPCR.
Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 26;26(11):5100. [Abstract]
N2a cells were mock-treated or treated with Sodium orthovanadate (Na3VO4, 40 μM) for 1 h at 37 ℃ and then infected with CVS-11 at an MOI of 1 for 24 h. The cells were lysed to determine the RNA copy numbers of RABV N by and detect the protein level of RABV N by western blot.
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Vet Microbiol
Dynein- and kinesin- mediated intracellular transport on microtubules facilitates RABV infection. [Abstract]2021 Nov:262:109241. PMID: 34555731 -
Tissue Cell
SOV sensitizes gastric cancer cells to radiation by suppressing radiation-induced autophagy in vitro and in vivo. [Abstract]2023 Jun:82:102109. PMID: 37229935
Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Tissue Cell. 2023 Jun:82:102109. [Abstract]
Sodium orthovanadate (SOV; 0, 2, 4, 8, 16, 32 µM; 48 h) significantly suppresses the cell viability of SGC-7901 and MGC-803 in a dose-dependent manner.
Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Tissue Cell. 2023 Jun:82:102109. [Abstract]
Sodium orthovanadate (SOV; 0, 2, 4, 8, 16, 32 µM; 48 h) significantly suppresses the proliferative capacity of SGC-7901 and MGC-803 in a dose-dependent manner.
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bioRxiv
2024 Jul 12:2024.07.10.602941. PMID: 39026771
Solvent & Solubility
H2O : 8 mg/mL (43.50 mM; ultrasonic and warming and heat to 60°C)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 16.67 mg/mL (90.64 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Gordon JA, et al. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods Enzymol. 1991;201:477-82. [Content Brief]
[2]. Wu DN, et al. Down-regulation of PTEN by sodium orthovanadate inhibits ASK1 activation via PI3-K/Akt during cerebral ischemia in rat hippocampus. Neurosci Lett. 2006 Aug 14;404(1-2):98-102. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 5.4374 mL | 27.1872 mL | 54.3744 mL | 135.9361 mL |
| 5 mM | 1.0875 mL | 5.4374 mL | 10.8749 mL | 27.1872 mL | |
| 10 mM | 0.5437 mL | 2.7187 mL | 5.4374 mL | 13.5936 mL | |
| 15 mM | 0.3625 mL | 1.8125 mL | 3.6250 mL | 9.0624 mL | |
| 20 mM | 0.2719 mL | 1.3594 mL | 2.7187 mL | 6.7968 mL | |
| 25 mM | 0.2175 mL | 1.0875 mL | 2.1750 mL | 5.4374 mL | |
| 30 mM | 0.1812 mL | 0.9062 mL | 1.8125 mL | 4.5312 mL | |
| 40 mM | 0.1359 mL | 0.6797 mL | 1.3594 mL | 3.3984 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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