HyT36
Based on 1 Customer Validation
HyT36 is a low molecular weight hydrophobic tag that promotes the degradation of fusion protein and pseudokinase Her3.
For research use only. We do not sell to patients.
- Purity: 96.0%
- CAS No.: 1323151-45-6
- Formula: C25H44ClNO3
- Molecular Weight:442.07
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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JAK/STAT Compound Library
HY-L008
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Protein Tyrosine Kinase Compound Library
HY-L016
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Anti-Cancer Compound Library
HY-L025
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Anti-Hepatitis C Virus Compound Library
HY-L073
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Anti-Pancreatic Cancer Compound Library
HY-L077
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Angiogenesis-Related Compound Library
HY-L088
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Membrane Protein-targeted Compound Library
HY-L149
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Membrane Receptor-targeted Compound Library
HY-L150
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Protein Kinase Compound Library
HY-L196
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
All EGFR Isoforms
More
Biological Activity
HyT36 (10 μM; 24 h) decreases the expression of GFP-HaloTag2/Fz4-HaloTag2/GFP-HaloTag7 fusion proteins in HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2[1].
HyT36 (10 μM; 2 h or 48 h) reduces the expression of EGFP and increases the binding of Hsp70 and HA-EGFP-HaloTag2 fusion protein in HEK293 Flp-In cells expressing HA-EGFP-HaloTag2[3].
HyT36 (50 nM; 24 h) reduces the level of EGFP-HaloTag2 in HEK293 Flp-In cells expressing HA-EGFP-HaloTag2[3].
HyT36 (1 μM; 4 d) decreases the expression of HA HaloTag2 HRasG12V in NIH-3T3 cells expressing HA HaloTag2 HRasG12V[3]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2/HA-EGFP-HaloTag2 or NIH-3T3 cells expressing HA−HaloTag2−HRasG12V.
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Concentration:1 µM or 10 µM.
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Incubation Time:2 h, 24 h or 4 days.
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Result:Decreased the expression of fusion proteins and increased the binding of Hsp70 and HA-EGFP-HaloTag2 fusion protein.
Chemical Information
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CAS No. 1323151-45-6
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Appearance Oil
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Molecular Weight 442.07
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Formula C25H44ClNO3
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Color Light yellow to brown
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SMILES
ClCCCCCCOCCOCCNC([C@H](C)CCC1(C[C@H]2C3)C[C@H]3C[C@H](C2)C1)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (226.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Tae HS, et al. Identification of hydrophobic tags for the degradation of stabilized proteins. Chembiochem. 2012 Mar 5;13(4):538-41. [Content Brief]
[2]. Rubner S, et al. Poloxin-2HT+: changing the hydrophobic tag of Poloxin-2HT increases Plk1 degradation and apoptosis induction in tumor cells. Org Biomol Chem. 2019 Mar 20;17(12):3113-3117. [Content Brief]
[3]. Neklesa TK, et al. A bidirectional system for the dynamic small molecule control of intracellular fusion proteins. ACS Chem Biol. 2013 Oct 18;8(10):2293-2300. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2621 mL | 11.3104 mL | 22.6209 mL | 56.5521 mL |
| 5 mM | 0.4524 mL | 2.2621 mL | 4.5242 mL | 11.3104 mL | |
| 10 mM | 0.2262 mL | 1.1310 mL | 2.2621 mL | 5.6552 mL | |
| 15 mM | 0.1508 mL | 0.7540 mL | 1.5081 mL | 3.7701 mL | |
| 20 mM | 0.1131 mL | 0.5655 mL | 1.1310 mL | 2.8276 mL | |
| 25 mM | 0.0905 mL | 0.4524 mL | 0.9048 mL | 2.2621 mL | |
| 30 mM | 0.0754 mL | 0.3770 mL | 0.7540 mL | 1.8851 mL | |
| 40 mM | 0.0566 mL | 0.2828 mL | 0.5655 mL | 1.4138 mL | |
| 50 mM | 0.0452 mL | 0.2262 mL | 0.4524 mL | 1.1310 mL | |
| 60 mM | 0.0377 mL | 0.1885 mL | 0.3770 mL | 0.9425 mL | |
| 80 mM | 0.0283 mL | 0.1414 mL | 0.2828 mL | 0.7069 mL | |
| 100 mM | 0.0226 mL | 0.1131 mL | 0.2262 mL | 0.5655 mL |
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