Identification of hydrophobic tags for the degradation of stabilized proteins

  • Chembiochem. 2012 Mar 5;13(4):538-41. doi: 10.1002/cbic.201100793.
Hyun Seop Tae  1 ,  Thomas B Sundberg ,  Taavi K Neklesa ,  Devin J Noblin ,  Jeffrey L Gustafson ,  Anke G Roth ,  Kanak Raina ,  Craig M Crews
Affiliations
  • 1. Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511, USA.
PMID: 22271667 DOI: 10.1002/cbic.201100793
Abstract

New HyTs are a knockout: we previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.

Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-141676
    HyT36
    Hydrophobic Tag
    target: EGFR
    Research Areas: Others