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HyT36 

Cat. No.: HY-141676
Handling Instructions

HyT36 is a low molecular weight hydrophobic tag that promotes the degradation of fusion protein and pseudokinase Her3.

For research use only. We do not sell to patients.

HyT36 Chemical Structure

HyT36 Chemical Structure

CAS No. : 1323151-45-6

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 165 In-stock
Estimated Time of Arrival: December 31
Oil + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 165 In-stock
Estimated Time of Arrival: December 31
Oil
5 mg USD 150 In-stock
Estimated Time of Arrival: December 31
10 mg USD 250 In-stock
Estimated Time of Arrival: December 31
25 mg USD 550 In-stock
Estimated Time of Arrival: December 31
50 mg USD 950 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1550 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

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Description

HyT36 is a low molecular weight hydrophobic tag that promotes the degradation of fusion protein and pseudokinase Her3[1][2][3].

In Vitro

HyT36 (10 µM; 24 h) decreases the expression of GFP-HaloTag2/Fz4-HaloTag2/GFP-HaloTag7 fusion proteins in HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2[1].
HyT36 (10 µM; 2 h or 48 h) reduces the expression of EGFP and increases the binding of Hsp70 and HA-EGFP-HaloTag2 fusion protein in HEK293 Flp-In cells expressing HA-EGFP-HaloTag2[3].
HyT36 (50 nM; 24 h) reduces the level of EGFP-HaloTag2 in HEK293 Flp-In cells expressing HA-EGFP-HaloTag2[3].
HyT36 (1 µM; 4 d) decreases the expression of HA−HaloTag2−HRasG12V in NIH-3T3 cells expressing HA−HaloTag2−HRasG12V[3]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1][3]

Cell Line: HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2/HA-EGFP-HaloTag2 or NIH-3T3 cells expressing HA−HaloTag2−HRasG12V.
Concentration: 1 µM or 10 µM.
Incubation Time: 2 h, 24 h or 4 days.
Result: Decreased the expression of fusion proteins and increased the binding of Hsp70 and HA-EGFP-HaloTag2 fusion protein.
Molecular Weight

442.07

Appearance

Oil

Formula

C25H44ClNO3
CAS No.
SMILES

ClCCCCCCOCCOCCNC([[email protected]](C)CCC1(C[[email protected]]2C3)C[[email protected]]3C[[email protected]](C2)C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (226.21 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2621 mL 11.3104 mL 22.6209 mL
5 mM 0.4524 mL 2.2621 mL 4.5242 mL
10 mM 0.2262 mL 1.1310 mL 2.2621 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

HyT361323151-45-6HyT 36HyT-36OthersHEK293NIH-3T3GFP-HaloTag2Fz4-HaloTag2GFP-HaloTag7HA-EGFP-HaloTag2HA?HaloTag2?HRasG12VInhibitorinhibitorinhibit

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