Cat. No.: HY-D1434

FM1-43

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

FM1-43 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers.

For research use only. We do not sell to patients.

Purity: 99.85%
CAS No.: 149838-22-2
Formula: C₃₀H₄₉Br₂N₃
Molecular Weight:611.54

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

FM1-43 Chemical Structure

CAS No.: 149838-22-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. FM Working Fluid Preparation 1.1 Preparation of Storage Solution Prepare a 5 mM stock solution using DMSO.
1.2 Preparation of Working Solution Prepare the FM 1-43 working solution at a concentration of 5-20 μM using the preheated HBSS solution.
Note: Please adjust the concentration of the FM 1-43 working solution according to the actual situation, and prepare it as needed.

2. Cell Staining (Suspension Cells) 2.1 Centrifuge the cells and add PBS for two washes, each for 5 minutes. The cell density is 1×10⁶ cells/mL. 2.2 Add 1 mL of FM working solution and incubate at room temperature for 5 to 30 minutes.
2.3 400 grams, centrifuge for 3-4 minutes, then discard the supernatant.
2.4 Add PBS to wash the cells twice, for 5 minutes each time.
2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
3. Cytological staining (adherent cells) 3.1 Cultivate adherent cells on a sterile cover glass.
3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.
3.4 Remove the dye working solution and wash with the culture medium 2-3 times, for 5 minutes each time. Then observe using a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

585

Excitation (Ex)

480

Chemical Information

CAS No. 149838-22-2
Appearance Solid
Molecular Weight 611.54
Formula C₃₀H₄₉Br₂N₃
Color Orange to red
SMILES

CC[N+](CC)(CC)CCC[N+](C=C1)=CC=C1/C=C/C2=CC=C(N(CCCC)CCCC)C=C2.[Br-].[Br-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (8)

All Cell Imaging/StainingBio/Physico-chemical Assay

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Journal Impact Factor

J Extracell Vesicles

Unveiling a Novel Mechanism of Enhanced Secretion, Cargo Loading, and Accelerated Dynamics of Bacterial Extracellular Vesicles Following Antibiotic Exposure. [Abstract]

2025 Jul;14(7):e70131. PMID: 40704571
Mol Cell

Longitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages. [Abstract]

2026 Jun 25:S1097-2765(26)00378-3. PMID: 42349405
Adv Sci (Weinh)

Bioinspired Engineering of Streamlined Skeletal Interoception: Neural Bioprinted Piezoelectric Scaffolds for Neuro-Vascularized Bone Regeneration. [Abstract]

2026 Apr;13(21):e24181. PMID: 41691467
Microbiol Res

Inappropriate use of antibiotic enhances antibiotic resistance dissemination in ESBL-EC: Role of ydcz in outer membrane vesicles biogenesis and protein transport. [Abstract]

2024 Aug:285:127774. PMID: 38833829

FM1-43 purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2024 Aug:285:127774. [Abstract]

FM1-43 (1 μM; 30 min). OMVs were observed by CLSM, the white arrow pointing OMVs.
Curr Biol

A surfactant lipid layer of endosomal membranes facilitates airway gas filling in Drosophila. [Abstract]

2023 Dec 4;33(23):5132-5146.e5. PMID: 37992718
Development

Rbm24 promotes outer hair cell survival through Insm1 repression while independently regulating hair bundle morphogenesis. [Abstract]

2026 Apr 15;153(8):dev204234. PMID: 41884985
SSRN

2025 Jun 4.

FM1-43 purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Jun 4.

Fluorescence in isolated organs of Corti from WT and Tmem145^-/- mice (age: P14-P21) was observed at 180 s of incubation with FM1-43 (10 μM; 170 s). Right, pre-incubation with BAPTA (5 mM, 20 min) abolished FM1-43 uptake.

FM1-43 purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Jun 4.

Time course of FM1-43 (10 μM) dye uptake by WT (black) and Tmem145^-/- (red) OHCs.
Sci Total Environ

Bacterial membrane vesicles from swine farm microbial communities harboring and safeguarding diverse functional genes promoting horizontal gene transfer. [Abstract]

2024 Nov 15:951:175639. PMID: 39168346

FM1-43 purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 Nov 15:951:175639. [Abstract]

FM1-43 (1 μM; 37 ℃; 30 min). BMVs were observed by SR-CLSM. S1. The red arrow pointed to the first secreted vesicle, and the white arrow pointed to the second one. Inside the white circle were the already secreted BMVs.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (81.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6352 mL

8.1761 mL

16.3522 mL

5 mM

0.3270 mL

1.6352 mL

3.2704 mL

10 mM

0.1635 mL

0.8176 mL

1.6352 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. J E Gale, et al. FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel. J Neurosci. 2001 Sep 15;21(18):7013-25. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6352 mL	8.1761 mL	16.3522 mL	40.8804 mL
	5 mM	0.3270 mL	1.6352 mL	3.2704 mL	8.1761 mL
	10 mM	0.1635 mL	0.8176 mL	1.6352 mL	4.0880 mL
	15 mM	0.1090 mL	0.5451 mL	1.0901 mL	2.7254 mL
	20 mM	0.0818 mL	0.4088 mL	0.8176 mL	2.0440 mL
	25 mM	0.0654 mL	0.3270 mL	0.6541 mL	1.6352 mL
	30 mM	0.0545 mL	0.2725 mL	0.5451 mL	1.3627 mL
	40 mM	0.0409 mL	0.2044 mL	0.4088 mL	1.0220 mL
	50 mM	0.0327 mL	0.1635 mL	0.3270 mL	0.8176 mL
	60 mM	0.0273 mL	0.1363 mL	0.2725 mL	0.6813 mL
	80 mM	0.0204 mL	0.1022 mL	0.2044 mL	0.5110 mL

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FM1-43

Publications Citing Use of MedChemExpress (MCE) FM1-43

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

FM1-43 Related Classifications

HY-D1434 Related Classifications