2,4-Difluorobenzaldehyde
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2,4-Difluorobenzaldehyde is a fluorinated benzaldehyde derivative commonly used as an intermediate in organic synthesis. 2,4-Difluorobenzaldehyde serves as a substrate for enzymatic oxidation and undergoes the Baeyer-Villiger oxidation reaction under the catalysis of 4-hydroxyacetophenone monooxygenase. 2,4-Difluorobenzaldehyde acts as a synthetic precursor for trichalcone enzyme inhibitors. 2,4-Difluorobenzaldehyde is applicable to related research in fields such as medicine, pesticides, and liquid crystal materials.
For research use only. We do not sell to patients.
- Purity: 99.51%
- CAS No.: 1550-35-2
- Formula: C7H4F2O
- Molecular Weight:142.10
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
2,4-Difluorobenzaldehyde reacts with 1,1',1''-(2,4,6-trimethoxybenzene-1,3,5-triyl) triacetone to produce a trichalcone derivative[1].
2,4-Difluorobenzaldehyde undergoes Baeyer-Villiger oxidation catalyzed by purified HAPMO from Pseudomonas fluorescens ACB without substrate inhibition, with an apparent K'm of 5.7 mM and an apparent k'cat of 2.3 s−1[2].
2,4-Difluorobenzaldehyde undergoes HAPMO-mediated transformation at pH 8.0, with 95% of the product being 2,4-difluorophenol and 5% being 2,4-difluorobenzoic acid[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1550-35-2
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Appearance Liquid (Density: 1.299 g/cm3)
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Molecular Weight 142.10
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Formula C7H4F2O
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Color Colorless to light yellow
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SMILES
FC1=CC(F)=C(C=O)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (703.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (605 KB)
- English - EN (605 KB)
- Français - FR (605 KB)
- Deutsch - DE (605 KB)
- Norwegian - NO (605 KB)
- Español - ES (605 KB)
- Swedish - SV (605 KB)
- Italian - IT (605 KB)
- Korean - KR (605 KB)
- Portuguese - PT (605 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.0373 mL | 35.1865 mL | 70.3730 mL | 175.9324 mL |
| 5 mM | 1.4075 mL | 7.0373 mL | 14.0746 mL | 35.1865 mL | |
| 10 mM | 0.7037 mL | 3.5186 mL | 7.0373 mL | 17.5932 mL | |
| 15 mM | 0.4692 mL | 2.3458 mL | 4.6915 mL | 11.7288 mL | |
| 20 mM | 0.3519 mL | 1.7593 mL | 3.5186 mL | 8.7966 mL | |
| 25 mM | 0.2815 mL | 1.4075 mL | 2.8149 mL | 7.0373 mL | |
| 30 mM | 0.2346 mL | 1.1729 mL | 2.3458 mL | 5.8644 mL | |
| 40 mM | 0.1759 mL | 0.8797 mL | 1.7593 mL | 4.3983 mL | |
| 50 mM | 0.1407 mL | 0.7037 mL | 1.4075 mL | 3.5186 mL | |
| 60 mM | 0.1173 mL | 0.5864 mL | 1.1729 mL | 2.9322 mL | |
| 80 mM | 0.0880 mL | 0.4398 mL | 0.8797 mL | 2.1992 mL | |
| 100 mM | 0.0704 mL | 0.3519 mL | 0.7037 mL | 1.7593 mL |
2,4-Difluorobenzaldehyde Related Classifications
HY-W007410 Related Classifications
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