Methacholine chloride
Based on 6 publication(s) in Google Scholar
Methacholine (Acetyl-β-methylcholine) choride is a potent muscarinic-3 (M3) agonist. Methacholine choride acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine choride shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine choride can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 62-51-1
- Formula: C8H18ClNO2
- Molecular Weight:195.69
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Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Methacholine chloride
More- Cell Death Dis. 2025 Oct 7;16(1):716. [Abstract]
- J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]
- J Ethnopharmacol. 2025 Oct 22:356:120782. [Abstract]
- Aging (Albany NY). 2021 Sep 13;13(17):21729-21742. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2025 Nov 12. [Abstract]
- Immunobiology. 2025 Jul 16;230(4):153103. [Abstract]
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
Biological Activity
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mAChR3 |
Methacholine choride (0.5 mg/kg; i.v.) induces bronchoconstriction was inhibited by bradykinin (4-40 μg/kg; i.v.) in a a dose-dependent manner in mouse[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:9-week female BALB/c mice[6]
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Dosage:0.03, 0.1, 0.3, 1 mg/kg
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Administration:I.v.
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Result:Induced severe bronchoconstriction.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 62-51-1
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Appearance Solid
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Molecular Weight 195.69
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Formula C8H18ClNO2
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Color White to off-white
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SMILES
CC(OC(C)=O)C[N+](C)(C)C.[Cl-]
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Synonyms
Acetyl-β-methylcholine chloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Publications (6)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
E3 Ubiquitin ligases Cbl-b and c-Cbl maintain the homeostasis of macrophages by regulating the M-CSF/M-CSFR signaling axis. [Abstract]2025 Oct 7;16(1):716. PMID: 41057360
Methacholine chloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Oct 7;16(1):716. [Abstract]
Methacholine chloride (6.25-50 mg/mL; i.n.; 5 min). The breathing frequency (f), inspiratory time (Ti), expiratory time (Te), tidal volume, baseline (TVb), and expiratory end pressure (EEP) of dKO mice were measured by the WBP system.
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J Ethnopharmacol
YiQi GuBen formula targets EHMT2 and enhances NEDD4L transcription to combat epithelial-mesenchymal transition and airway remodeling in asthma. [Abstract]2026 Mar 25:359:121125. PMID: 41475628
Methacholine chloride purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]
AHR of OVA-sensitized BALB/c mice with or without YQGBF treatment was examined via Methacholine chloride (10-30 mg/mL; 3 min) exposure.
Methacholine chloride purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]
AHR of OVA-sensitized BALB/c mice with or without EHMT2 overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.
Methacholine chloride purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]
AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and NEDD4L overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.
Methacholine chloride purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]
AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and treated with Wnt/β-catenin inhibitor ICG-001 was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.
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J Ethnopharmacol
Network pharmacology and experimental validation identify the targets of Marsdenia tenacissima in neutrophilic asthma treatment. [Abstract]2025 Oct 22:356:120782. PMID: 41135618 -
Aging (Albany NY)
2021 Sep 13;13(17):21729-21742. PMID: 34516405 -
Naunyn Schmiedebergs Arch Pharmacol
Lactobacillus rhamnosus regulates airway epithelial cell senescence through the ADCK5/PI3K/AKT signaling axis and alleviates airway inflammation in asthma. [Abstract]2025 Nov 12. PMID: 41222693 -
Immunobiology
Exosomes derived from baicalin-pretreated bone marrow mesenchymal stem cells inactivate the TLR4/MyD88/NF-kB pathway to improve asthma. [Abstract]2025 Jul 16;230(4):153103. PMID: 40695068
Solvent & Solubility
DMSO : 100 mg/mL (511.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (511.01 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 130 mg/mL (664.32 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Cohen J, et al. Relationship between airway responsiveness to neurokinin A and methacholine in asthma. Pulm Pharmacol Ther. 2005;18(3):171-176. [Content Brief]
[2]. Anderson SD, et al. Comparison of mannitol and methacholine to predict exercise-induced bronchoconstriction and a clinical diagnosis of asthma. Respir Res. 2009;10(1):4. Published 2009 Jan 23. [Content Brief]
[3]. Cockcroft DW. Methacholine challenge methods. Chest. 2008;134(4):678-680. [Content Brief]
[4]. Kabara S, et al. Differential effects of thiopental on methacholine- and serotonin-induced bronchoconstriction in dogs. Br J Anaesth. 2003 Sep;91(3):379-84. [Content Brief]
[5]. Folkerts G, et al. Bradykinin causes inhibition of methacholine-induced bronchoconstriction in vivo in mice. Naunyn Schmiedebergs Arch Pharmacol. 2001 Jul;364(1):53-8. [Content Brief]
[6]. Vitorasso RL, et al. Methacholine dose response curve and acceptability criteria of respiratory mechanics modeling. Exp Lung Res. 2020 Feb-Mar;46(1-2):23-31. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 5.1101 mL | 25.5506 mL | 51.1012 mL | 127.7531 mL |
| 5 mM | 1.0220 mL | 5.1101 mL | 10.2202 mL | 25.5506 mL | |
| 10 mM | 0.5110 mL | 2.5551 mL | 5.1101 mL | 12.7753 mL | |
| 15 mM | 0.3407 mL | 1.7034 mL | 3.4067 mL | 8.5169 mL | |
| 20 mM | 0.2555 mL | 1.2775 mL | 2.5551 mL | 6.3877 mL | |
| 25 mM | 0.2044 mL | 1.0220 mL | 2.0440 mL | 5.1101 mL | |
| 30 mM | 0.1703 mL | 0.8517 mL | 1.7034 mL | 4.2584 mL | |
| 40 mM | 0.1278 mL | 0.6388 mL | 1.2775 mL | 3.1938 mL | |
| 50 mM | 0.1022 mL | 0.5110 mL | 1.0220 mL | 2.5551 mL | |
| 60 mM | 0.0852 mL | 0.4258 mL | 0.8517 mL | 2.1292 mL | |
| 80 mM | 0.0639 mL | 0.3194 mL | 0.6388 mL | 1.5969 mL | |
| 100 mM | 0.0511 mL | 0.2555 mL | 0.5110 mL | 1.2775 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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