STAT4 Antibody (YA055)
(別名: STAT4; Signal transducer and activator of transcription 4)Based on 1 Customer Validation
STAT4 Antibody (YA055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human
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Formulation:
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Antibody Dilution Buffer (for Immunohistochemistry, IHC)
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Antibody Dilution Buffer (for Western Blot)
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TBS-T Powder (1 L of 1×)
HY-K1025
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PBS Buffer (1×)
HY-K3005
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DAB Peroxidase Substrate Kit (Purple-Blue Color)
HY-K1107
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Hematoxylin-Eosin Staining Kit
HY-K0315
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AntiFade Mounting Medium (with DAPI)
HY-K1047
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3-Color Prestained Protein Marker (10-190 kDa)
HY-K1011
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PBS-T Powder (1 L of 1×)
HY-K1022
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AntiFade Reagent (with DAPI)
HY-K1048
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Ultra High Sensitivity ECL Kit
HY-K1005
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TBS Powder (1 L of 1×)
HY-K1026
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High Sensitivity ECL Kit
HY-K2005
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Penicillin-Streptomycin (100×), Sterile
HY-K1006
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GelHP Protein Gel (Tris-Gly, 12%, 10 well)
HY-K1113
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PMSF (100×)
HY-K0004
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Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O)
HY-K0013
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DPBS Buffer (1×)
HY-K3008
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GelHP Protein Gel (Tris-Gly, 10%, 15 well)
HY-K1112
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Ponceau Staining Solution (Ready-to-use, 1×)
HY-K1109
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GelHP Protein Gel (Tris-Gly, 10%, 10 well)
HY-K1111
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Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)
HY-K0011
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
STAT4 Antibody (YA055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT4.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 86 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 86 kDa
Synthetic peptide corresponding to Human STAT4.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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濃度
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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輸送条件
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and SiHa(lane 4(20ug)using STAT4 Antibody (HY-P80903) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
STAT4 transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35. Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter. In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Cytoplasm; Nucleus
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Subunit
Forms a homodimer or a heterodimer with a related family member. Interacts with ARL2BP (By similarity). The SH2 domain interacts, in vitro, with IL12RB2 via a short cytoplasmic domain. Interacts with STAT1 (PubMed:34508746). Interacts with JUN; this complex efficiently interacts with the AP-1-related sequence of the IFN-gamma (By similarity)
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SwissProt ID
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別名
STAT4; Signal transducer and activator of transcription 4
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Research Field
Signal Transduction
ドキュメント
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データシート (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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抗体ユーザーガイドラインです (1077 KB)
[1]. Visconti R, et al. Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity. Blood. 2000 Sep 1;96(5):1844-52. [Content Brief]
[2]. Baghdassarian H, et al. Variant STAT4 and Response to Ruxolitinib in an Autoinflammatory Syndrome. N Engl J Med. 2023 Jun 15;388(24):2241-2252. [Content Brief]
[3]. Cho SS, et al. Activation of STAT4 by IL-12 and IFN-alpha: evidence for the involvement of ligand-induced tyrosine and serine phosphorylation. J Immunol. 1996 Dec 1;157(11):4781-9. [Content Brief]
[4]. Morinobu A, et al. STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation. Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12281-6. [Content Brief]
[5]. Zhang YS, et al. Acetylation licenses Th1 cell polarization to constrain Listeria monocytogenes infection. Cell Death Differ. 2022 Nov;29(11):2303-2315. [Content Brief]
[6]. Yao BB, et al. Direct interaction of STAT4 with the IL-12 receptor. Arch Biochem Biophys. 1999 Aug 1;368(1):147-55. [Content Brief]
[7]. Bacon CM, et al. Interleukin 12 induces tyrosine phosphorylation and activation of STAT4 in human lymphocytes. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7307-11. [Content Brief]
[8]. Oppmann B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity. 2000 Nov;13(5):715-25. [Content Brief]
[9]. Li M, et al. Interleukin-35 inhibits lipopolysaccharide-induced endothelial cell activation by downregulating inflammation and apoptosis. Exp Cell Res. 2021 Oct 15;407(2):112784. [Content Brief]
[10]. Gonzales-van Horn SR, et al. STAT4-mediated transcriptional repression of the IL5 gene in human memory Th2 cells. Eur J Immunol. 2016 Jun;46(6):1504-10. [Content Brief]
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