Citraconic anhydride
Based on 1 Customer Validation
Citraconic anhydride (Methylmaleic anhydride) is a derivative of maleic anhydride (HY-Z0060) and novel antigen retrieval solution. Citraconic anhydride reversibly blocks protein amino groups, stabilizing specific enzymes and improving their catalytic performance. Citraconic anhydride reacts with free amino groups on proteins (especially lysine residues), converting positively charged NH3+ into carboxyl groups, thereby disrupting methylene bridge crosslinks caused by Formaldehyde during antigen retrieval. Citraconic anhydride functionalizes Isotactic polypropylene. Citraconic anhydride precisely responds to pH changes to achieve reversible modification. Citraconic anhydride is irritating to skin and eyes.
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- Purity: 99.81%
- CAS No.: 616-02-4
- 화학식: C5H4O3
- 분자량:112.08
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보관:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
Chemical Information
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CAS No. 616-02-4
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Appearance Liquid (Density: 1.2469 g/cm³)
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분자량 112.08
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화학식 C5H4O3
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Color Colorless to light yellow
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SMILES
O=C1OC(C(C)=C1)=O
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Synonyms
Methylmaleic anhydride
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
DMSO : ≥ 100 mg/mL (892.22 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.31 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% Saline
Solubility: ≥ 2.5 mg/mL (22.31 mM); Clear solution
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (275 KB)
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SDS (640 KB)
- English - EN (640 KB)
- Français - FR (640 KB)
- Deutsch - DE (640 KB)
- Norwegian - NO (640 KB)
- Español - ES (640 KB)
- Swedish - SV (640 KB)
- Italian - IT (640 KB)
- Korean - KR (640 KB)
- Portuguese - PT (640 KB)
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Handling Instructions (2659 KB)
References
[3]. Yankeelov JA Jr, et al. Methylmaleic anhydride as a reversible blocking agent during specific arginine modification. Biochem Biophys Res Commun. 1971 Mar 5;42(5):886-91. [Content Brief]
[4]. Melodia D, et al. Traceless pH-Sensitive Antibody Conjugation Inspired by Citraconic Anhydride. Biomacromolecules. 2022 Dec 12;23(12):5322-5329. [Content Brief]
[5]. Leong AS, et al. Citraconic anhydride: a new antigen retrieval solution. Pathology. 2010 Jan;42(1):77-81. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 8.9222 mL | 44.6110 mL | 89.2220 mL | 223.0550 mL |
| 5 mM | 1.7844 mL | 8.9222 mL | 17.8444 mL | 44.6110 mL | |
| 10 mM | 0.8922 mL | 4.4611 mL | 8.9222 mL | 22.3055 mL | |
| 15 mM | 0.5948 mL | 2.9741 mL | 5.9481 mL | 14.8703 mL | |
| 20 mM | 0.4461 mL | 2.2305 mL | 4.4611 mL | 11.1527 mL | |
| 25 mM | 0.3569 mL | 1.7844 mL | 3.5689 mL | 8.9222 mL | |
| 30 mM | 0.2974 mL | 1.4870 mL | 2.9741 mL | 7.4352 mL | |
| 40 mM | 0.2231 mL | 1.1153 mL | 2.2305 mL | 5.5764 mL | |
| 50 mM | 0.1784 mL | 0.8922 mL | 1.7844 mL | 4.4611 mL | |
| 60 mM | 0.1487 mL | 0.7435 mL | 1.4870 mL | 3.7176 mL | |
| 80 mM | 0.1115 mL | 0.5576 mL | 1.1153 mL | 2.7882 mL | |
| 100 mM | 0.0892 mL | 0.4461 mL | 0.8922 mL | 2.2305 mL |
Citraconic anhydride Related Classifications
HY-78035 Related Classifications
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