L-Lactate dehydrogenase, E.coli
Based on 1 Customer Validation
L-Lactate dehydrogenase, E.coli is a redox enzyme. L-Lactate dehydrogenase, E.coli catalyzes the reduction of pyruvate to L-lactate by NADH in vivo with absolute enantiospecificity.
For research use only. We do not sell to patients.
- CAS No.: 9001-60-9
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Reaction conditions
Molecular weight:38 kDa (SDS-PAGE)
Isoelectric point: 6.2
Optimum pH: 6.5
Optimum temperature: 45°C
pH Stability: 4.5-10.0 (37°C, 1h)
Thermal stability: <50°C (pH 7.4, 15 min)
Inhibitors: Co2+, Cu2+, Fe3+, Ni2+, Zn2+, NEM, SDS, Proclin
Protocol
The enzyme dissolved in water.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
1.1.1.27
≥30 U/mg soild
One unit is defined as the amount of enzyme that reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37°C.
Chemical Information
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CAS No. 9001-60-9
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Appearance Solid
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Color White to off-white
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SMILES
[L-Lactate dehydrogenase, E.coli]
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Synonyms
L-LDH
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Simon ES, et al. D-lactate dehydrogenase. Substrate specificity and use as a catalyst in the synthesis of homochiral 2-hydroxy acids. Appl Biochem Biotechnol. 1989 Nov;22(2):169-79. [Content Brief]
[2]. Holmberg N, et al. Redesign of the coenzyme specificity in L-lactate dehydrogenase from bacillus stearothermophilus using site-directed mutagenesis and media engineering. Protein Eng. 1999 Oct;12(10):851-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
L-Lactate dehydrogenase, E.coli Related Classifications
HY-P2807 Related Classifications
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