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LCP is a fluorescent probe applicable for subcellular localization. LCP responds to polarity changes in the cellular microenvironment via fluorescence resonance energy transfer, emitting blue fluorescence in low-polarity environments and red fluorescence in high-polarity environments. LCP enables dual-color visualization of dynamic changes in lysosomes and cytoplasmic membranes during drug-induced cell apoptosis, and monitors cell viability through localization and emission color changes. LCP can be used in cancer research.

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LCP

LCP Estructura química

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LCP Related Antibodies

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Descripciòn

LCP is a fluorescent probe applicable for subcellular localization. LCP responds to polarity changes in the cellular microenvironment via fluorescence resonance energy transfer, emitting blue fluorescence in low-polarity environments and red fluorescence in high-polarity environments. LCP enables dual-color visualization of dynamic changes in lysosomes and cytoplasmic membranes during drug-induced cell apoptosis, and monitors cell viability through localization and emission color changes. LCP can be used in cancer research[1].

In Vitro

The fluorescent probe LCP (10 μM) shows polarity-dependent fluorescence emission shifts in cell-free solvent systems, with distinct absorption and fluorescence peaks responsive to changes in solvent polarity[1].
The fluorescent probe LCP (10 μM) distinguishes between lipid phases in cell-free GUV membrane models by emitting red fluorescence in Ld (high-polarity) phases and blue fluorescence in Lo (low-polarity) phases[1].
The fluorescent probe LCP (0-40 μM) has low cytotoxicity to HeLa cells at concentrations up to 40 μM, making it suitable for live cell imaging applications[1].
The fluorescent probe LCP (10 μM; 15 min) localizes to lysosomes in live HeLa cells (with a colocalization coefficient of 0.91 with LysoTracker Red) and to the plasma membrane in cisplatin-induced apoptotic HeLa cells (with colocalization coefficients of 0.83 and 0.88 with DiO)[1].
The fluorescent probe LCP (10 μM; 10 min) shifts from blue (lysosomal) to red (plasma membrane) fluorescence in a concentration-dependent manner in cisplatin-induced apoptotic HeLa cells, with blue fluorescence weakening and red fluorescence strengthening as cisplatin concentration increases[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LCP Related Antibodies

Immunofluorescence[1]

Cell Line: HeLa cells (cisplatin-induced apoptosis, time-dependent)
Concentration: 10 μM (LCP); 30 μM (cisplatin pre-treatment)
Incubation Time: 10 min (LCP incubation); 0-24 h (cisplatin pre-treatment)
Result: Emitted only blue fluorescence (lysosomal localization) in HeLa cells pre-treated with 30 μM cisplatin for 0 h (live cells).
Showed gradually decreased blue channel fluorescence (lysosomal) and increasingly prominent red channel fluorescence (plasma membrane localization) in cells pre-treated for 6, 12, 18, or 24 h.
Peso molecular

975.09

Fòrmula

C56H71IN4O3
SMILES

CCCCCCCCCCCC[N+]1=CC=C(C2=C1C=CC=C2)/C=C/C3=CC=C(C=C3)N4CCN(CC4)CCCCOC5=C(OC6=C(C5=O)C=CC=C6)C7=CC=C(C=C7)N(CC)CC.[I-]
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Room temperature in continental US; may vary elsewhere.
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LCP Related Classifications

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LCPFluorescent DyeLo phasescancerGUV membrane modelslysosomesHeLa cellsLd phasesplasma membraneLysoTracker Reddrug-induced apoptosiscisplatinInhibitorinhibitorinhibit

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