2. Academic Validation
  3. Hydrangenol Isolated from the Leaves of Hydrangea serrata Attenuates Wrinkle Formation and Repairs Skin Moisture in UVB-Irradiated Hairless Mice

Hydrangenol Isolated from the Leaves of Hydrangea serrata Attenuates Wrinkle Formation and Repairs Skin Moisture in UVB-Irradiated Hairless Mice

  • Nutrients. 2019 Oct 2;11(10):2354. doi: 10.3390/nu11102354.
Da-Bin Myung 1 2,  Hee-Soo Han 3 4,  Ji-Sun Shin 5,  Ji Yeon Park 6 7,  Han Jun Hwang 8 9,  Hyoung Ja Kim 10,  Hye Shin Ahn 11,  Sun Hee Lee 12,  Kyung-Tae Lee 13 14
Affiliations

Affiliations

  • 1 Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 2 Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 3 Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 4 Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 5 Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 6 Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 7 Department of Fundamental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 8 Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 9 Department of Fundamental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 10 Molecular Recognition Research Center, Materials and Life Science Research Division, Korea Institute of Science and Technology, Seoul 02792, Korea. [email protected].
  • 11 Department of New Material Development, COSMAXBIO, Seongnam 13486, Korea. [email protected].
  • 12 Department of New Material Development, COSMAXBIO, Seongnam 13486, Korea. [email protected].
  • 13 Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
  • 14 Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. [email protected].
PMID: 31581754 DOI: 10.3390/nu11102354
Abstract

Our previous study showed that hydrangenol isolated from Hydrangea serrata leaves exerts antiphotoaging activity in vitro. In this study, we determined its antiphotoaging effect in UVB-irradiated HR-1 hairless mice. We evaluated wrinkle formation, skin thickness, histological characteristics, and mRNA and protein expression using qRT-PCR and Western blot analysis in dorsal skins. Hydrangenol mitigated wrinkle formation, dorsal thickness, dehydration, and collagen degradation. Hydrangenol increased the expression of involucrin, filaggrin, and aquaporin-3 (AQP3) as well as hyaluronic acid (HA) production via hyaluronidase (HYAL)-1/-2 downregulation. Consistent with the recovery of collagen composition, the expression of Pro-COL1A1 was increased by hydrangenol. Matrix metalloproteinase (MMP)-1/-3, cyclooxygenase-2 (COX-2), and interleukin-6 (IL-6) expression was reduced by hydrangenol. Hydrangenol attenuated the phosphorylation of mitogen-activated protein kinases (MAPKs) including ERK and p38, activator protein 1 (AP-1) subunit, and signal transduction and activation of transcription 1 (STAT1). Hydrangenol upregulated the expression of nuclear factor-E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO-1), glutamate cysteine ligase modifier subunit (GCLM), and glutamate cysteine ligase catalysis subunit (GCLC). Taken together, our data suggest that hydrangenol can prevent wrinkle formation by reducing MMP and inflammatory cytokine levels and increasing the expression of moisturizing factors and antioxidant genes.

Keywords

AP-1; MAPK; MMPs; collagen; hydrangenol; photoaging; skin moisture; ultraviolet B; wrinkle formation.

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