Necroptosis inducer 2

Necroptosis inducer 2 (Compound 2d) (20 μM) binds selectively and strongly to Cu²⁺ and Cu⁺ in a cell-free system, with K_a values of 1.42 × 10⁴/M and 7.33 × 10³/M, respectively^[1].
Necroptosis inducer 2 (48 h) potently inhibits the proliferation of MDA-MB-231, 4T1, HeLa, HT29, A2780, and U-2 OS cancer cells with IC₅₀ values ranging from 4.1-10.6 μM, shows moderate activity against A549 cells (IC₅₀ = 29.4 μM), and exhibits reduced toxicity toward non-cancerous MCF-10A cells (IC₅₀ = 10.6 μM)^[1].
Necroptosis inducer 2 (10 μM; 44 h treatment) has its cytotoxicity rescued in MDA-MB-231 cells by exogenous Cu²⁺, confirming copper chelation is essential for its antiproliferative activity^[1].
Necroptosis inducer 2 (10-20 μM; 24 h) reduces intracellular chelatable copper levels in MDA-MB-231 cells in a concentration-dependent manner, as detected by CS1 confocal microscopy^[1].
Necroptosis inducer 2 (20 μM; 24 h) rescues MDA-MB-231 cells from CuCl₂-induced cytotoxicity, demonstrating potent copper-chelating activity in a cellular context^[1].
Necroptosis inducer 2 (5-20 μM; 24 h) elevates intracellular ROS levels and disrupts mitochondrial membrane potential in MDA-MB-231 cells in vitro in a concentration-dependent manner, and this effect is reversed by exogenous Cu^2+[1].
Necroptosis inducer 2 (10 μM; 44 h) induces cell death in MDA-MB-231 cells primarily via the necroptosis pathway, as only Necrostatin-1 (HY-15760) rescues cell viability^[1].
Necroptosis inducer 2 (5-20 μM; 24 h) upregulates the necroptosis markers p-MLKL and p-RIP3 in MDA-MB-231 cells in vitro in a concentration-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.