2. Neuronal Signaling
  3. Tau Protein
  4. NS101

NS101 is an anti-FAM19A5 antibody with an IC50 of 0.2 nM and a Kd of 111 pM, as well as blood-brain barrier permeability. NS101 binds to key amino acid residues of FAM19A5, thereby blocking the interaction of LRRC4B and disrupting the FAM19A5-LRRC4B complex. NS101 increases the dendritic spine density in hippocampal neurons and the prefrontal cortex, normalizes the dendritic spine elimination rate, elevates the frequencies of mEPSC and fEPSP, and promotes the transport of brain-derived FAM19A5 into the peripheral circulation. NS101 improves cognitive function in mouse models of Alzheimer's disease. NS101 can be used for research on Alzheimer's disease. The recommended isotype control is human IgG1 kappa (HY-P99001).

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NS101

NS101 Chemical Structure

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NS101 Related Antibodies

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Description

NS101 is an anti-FAM19A5 antibody with an IC50 of 0.2 nM and a Kd of 111 pM, as well as blood-brain barrier permeability. NS101 binds to key amino acid residues of FAM19A5, thereby blocking the interaction of LRRC4B and disrupting the FAM19A5-LRRC4B complex. NS101 increases the dendritic spine density in hippocampal neurons and the prefrontal cortex, normalizes the dendritic spine elimination rate, elevates the frequencies of mEPSC and fEPSP, and promotes the transport of brain-derived FAM19A5 into the peripheral circulation. NS101 improves cognitive function in mouse models of Alzheimer's disease. NS101 can be used for research on Alzheimer's disease. The recommended isotype control is human IgG1 kappa (HY-P99001)[1].

Isotype

Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity

Human
In Vitro

NS101 potently inhibits the binding of FAM19A5 to LRRC4B in a cell-free ELISA assay with an IC50 of 205.0 pM[1].
NS101 (50 nM) blocks FAM19A5-induced reductions in spine density and increases the density of functional, SYP-positive spines in cultured mouse primary hippocampal neurons[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NS101 Related Antibodies
In Vivo

NS101 (10-30 mg/kg; i.v., i.p.; weekly; 2-4 doses) restores mushroom spine density and synaptic balance by normalizing spine elimination rates, and improves spatial learning and memory in P301S tauopathy mice, with accompanying reduction in cortical pTau levels[1].
NS101 (10-30 mg/kg; i.v.; weekly; 4 weeks) restores synaptic activity (mEPSC frequency, fEPSP slope) and improves short-term memory in APP/PS1 amyloidopathy mice, independent of Aβ reduction[1].
NS101 (10-50 mg/kg; i.v.; single dose) crosses the blood-brain barrier in rats, engages with FAM19A5, and facilitates transport of brain-derived FAM19A5 to the peripheral circulation in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: P301S [B6.C3-Tg (Prnp-MAPT*P301S) PS19Vle/J] (male, female, 6-7 months old, tauopathy model); wild-type littermates[1]
Dosage: 10 mg/kg (prefrontal cortex spine density; cognitive testing); 30 mg/kg (dendritic spine dynamics)
Administration: i.v.; weekly; 4 weeks (prefrontal cortex spine density; cognitive testing); i.p.; weekly; 2 doses (dendritic spine dynamics)
Result: Restored mushroom spine density in the prefrontal cortex of P301S mice to wild-type levels, while thin and stubby spine densities remained unchanged.
Normalized the spine elimination rate in P301S mice, restoring the net spine change to wild-type levels.
Reduced latency to locate the platform, increased number of platform crossings, and increased target quadrant occupancy in Morris water maze, matching wild-type performance.
Reduced pTau levels in the cerebral cortex (but not hippocampus) of P301S mice.
Animal Model: APP/PS1 [B6.Cg-Tg (APPswe, PSEN1dE9) 85Dbo/J] (male, 13 months old, amyloidopathy model); wild-type littermates[1]
Dosage: 10-30 mg/kg
Administration: i.v.; weekly; 4 weeks
Result: Restored mEPSC frequency in APP/PS1 mice to wild-type levels, with a slight increase in mEPSC amplitude.
Enhanced fEPSP slopes and fiber volley amplitudes in hippocampal CA1 neurons.
Improved the spontaneous alternation rate of APP/PS1 mice to wild-type levels, while total arm entries remained unchanged across groups.
Did not alter Aβ levels in the cerebral cortex or hippocampus of APP/PS1 mice.
Animal Model: Sprague-Dawley[1]
Dosage: 10 mg/kg; 50 mg/kg
Administration: i.v.; single dose
Result: Was detected in plasma with a half-life of 7.5 days (10 mg/kg) and 11.7 days (50 mg/kg), and showed dose-dependent systemic exposure (AUC 10,336.3 h·µg/mL for 10 mg/kg; 54,415.0 h·µg/mL for 50 mg/kg).
Crossed the blood-brain barrier, peaking in the brain at 6 hours post-injection with a half-life of 11.0 days (10 mg/kg) and 8.3 days (50 mg/kg), and peaking in CSF at 36 hours with dose-dependent levels.
Increased plasma FAM19A5 levels sharply post-administration then gradually decreased over 28 days in a dose-dependent manner, while brain and CSF FAM19A5 levels decreased after an initial short-term increase.
Gene ID

25817  [NCBI]

Accession

NP_056196
Target

TAFA5/FAM19A5
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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NS101 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NS101NS 101NS-101Tau Proteinprefrontal cortexFAM19A5mEPSCrathippocampal neuronsmouseP301S tauopathy miceLRRC4BfEPSPAlzheimer's diseaseInhibitorinhibitorinhibit

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