PCL-2 

PCL-2 is a reactive oxygen species-responsive fluorescent probe that shows almost no response to biologically relevant reactive oxygen species other than hydrogen peroxide. PCL-2 reacts with hydrogen peroxide to release 6-hydroxy-2-cyanobenzothiazole. PCL-2 can be used for chemoselective imaging of hydrogen peroxide in in vitro models and acute inflammation mouse models. PCL-2 is applicable to studies related to acute inflammation^[1].

PCL-2 (5 μM; 5, 20, 40, or 60 min) selectively produces a ca. 50-fold increase in bioluminescent signal in response to H₂O₂, but not other biologically relevant ROS, in a cell-free system^[1].
PCL-2 (5 μM; 60 min) produces a linearly increasing bioluminescent signal in response to 1-100 μM H₂O₂ in a cell-free system^[1].
PCL-2 (25 μM; 2 h) produces a linearly increasing, statistically significant bioluminescent signal in response to 0-100 μM H₂O₂ in PC3M-luc cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PCL-2 (0.05 μmol; intraperitoneal injection) produces a dose-dependent, 10-fold maximum bioluminescent turn-on signal in response to exogenous hydrogen peroxide in healthy FVB-luc⁺ mice, which is attenuated by a hydrogen peroxide scavenger^[1].
PCL-2 (0.05 μmol; intraperitoneal injection) detects a ~3.7-fold increase in endogenous hydrogen peroxide (H₂O₂) levels during lipopolysaccharide-induced acute inflammation in female FVB-luc+ mice, with signal reduced by 55% following apocynin treatment^[1].
PCL-2 (0.05 μmol; intraperitoneal injection), in combination with IETDC, detects a ~2.7-fold increase in simultaneous endogenous hydrogen peroxide and caspase 8 activity during lipopolysaccharide-induced acute inflammation in female FVB-luc+ mice, with signal reduced by 30% following combined ascorbic acid and caspase inhibitor treatment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

310.14

C₁₅H₁₁BN₂O₃S

1421277-81-7

N#CC1=NC2=CC=C(C=C2S1)OCC3=CC=C(C=C3)B(O)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Van de Bittner GC, et al. Strategy for dual-analyte luciferin imaging: in vivo bioluminescence detection of hydrogen peroxide and caspase activity in a murine model of acute inflammation. J Am Chem Soc. 2013;135(5):1783-1795.  [Content Brief]