PCL-2
PCL-2 is a reactive oxygen species (ROS)-responsive fluorescent probe. PCL-2 reacts with ROS to release 6-hydroxy-2-cyanobenzothiazole (HCBT), which then reacts with D-cysteine to form firefly luciferin in situ. PCL-2 must be used in conjunction with IETDC (HY-D3169) to detect the co-existence of hydrogen peroxide and caspase 8. PCL-2 is applicable to studies related to acute inflammation.
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- CAS. Nr.: 1421277-81-7
- Formel: C15H11BN2O3S
- Molecular Weight:310.14
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
In Vitro
PCL-2 (5 μM; 5, 20, 40, or 60 min) selectively produces a ca. 50-fold increase in bioluminescent signal in response to H2O2, but not other biologically relevant ROS, in a cell-free system[1].
PCL-2 (5 μM; 60 min) produces a linearly increasing bioluminescent signal in response to 1-100 μM H2O2 in a cell-free system[1].
PCL-2 (25 μM; 2 h) produces a linearly increasing, statistically significant bioluminescent signal in response to 0-100 μM H2O2 in PC3M-luc cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
PCL-2 (0.05 μmol; i.p.) detects a ~3.7-fold increase in endogenous hydrogen peroxide (H2O2) levels during lipopolysaccharide-induced acute inflammation in female FVB-luc+ mice[1].
PCL-2 (0.05 μmol; i.p.), in combination with IETDC, detects a ~2.7-fold increase in simultaneous endogenous hydrogen peroxide and caspase 8 activity during LPS (HY-D1056)-induced acute inflammation in female FVB-luc+ mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:FVB-luc+ (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) (female, 2-5 months)[1]
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Dosage:0.05 μmol (co-administered with 0.05 μmol D-cysteine)
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Administration:intraperitoneal injection
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Result:Produced a ~3.7-fold increase in total photon flux in lipopolysaccharide-challenged mice compared to saline-treated control mice.
Reduced this signal by ~55% when co-administered with apocynin relative to signal from lipopolysaccharide alone.
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Animal Model:FVB-luc+ (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) (female, 2-5 months)[1]
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Dosage:0.05 μmol (co-administered with 0.05 μmol IETDC)
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Administration:intraperitoneal injection
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Result:Produced a ~2.7-fold increase in total photon flux in lipopolysaccharide-challenged mice compared to saline-treated control mice.
Reduced this signal by ~30% when co-administered with ascorbic acid and z-VD(OMe)-OPh relative to signal from lipopolysaccharide alone.
Chemical Information
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CAS. Nr. 1421277-81-7
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Molecular Weight 310.14
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Formel C15H11BN2O3S
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SMILES
N#CC1=NC2=CC=C(C=C2S1)OCC3=CC=C(C=C3)B(O)O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
PCL-2 Related Classifications
HY-D3168 Related Classifications
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Keywords
- PCL-2
- 1421277-81-7
- PCL2
- PCL 2
- Fluorescent Dye
- Reactive Oxygen Species (ROS)
- reactive oxygen species
- caspase 8
- hydrogen peroxide
- FVB-luc+ mice
- lipopolysaccharide-induced acute inflammation
- apocynin
- ascorbic acid
- murine models of acute inflammation
- 6-hydroxy-2-cyanobenzothiazole
- PC3M-luc cells
- Inhibitor
- inhibitor
- inhibit