IETDC 

IETDC is a caspase 8 probe substrate. IETDC is cleaved by activated caspase 8 to release D-cysteine. The D-cysteine released by IETDC binds to HCBT to generate firefly luciferin in situ, accompanied by a bioluminescent signal produced by H₂O₂-mediated release of 6-hydroxy-2-cyanobenzothiazole. IETDC is applicable to studies related to acute inflammation^[1].

IETDC (5 μM; 60 min) is selectively cleaved by recombinant caspase 8 but not caspase 3 or caspase 9 to release D-cysteine, which forms luciferin with HCBT to produce a ca. 27-fold increase in bioluminescent signal, and this response is fully inhibited by a pan-caspase inhibitor^[1].
IETDC (10 μM; 60 min) acts as part of an AND-type molecular logic gate, producing an ca. 18-fold increase in bioluminescent signal only when combined with PCL-2, hydrogen peroxide, and active caspase 8, with signal blocked by inhibition of either hydrogen peroxide-mediated PCL-2 cleavage or caspase 8-mediated IETDC cleavage^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IETDC (0.05 μmol; i.p.; single dose; co-administered with 0.05 μmol HCBT), co-administered with HCBT, produces an 18-fold bioluminescent signal increase in female FVB-luc+ mice with lipopolysaccharide-induced acute inflammation, which is 34% attenuated by pre-treatment with a pan-caspase inhibitor^[1].
IETDC (0.05 μmol; i.p.; single dose; co-administered with 0.05 μmol PCL-2), co-administered with PCL-2, produces a 2.7-fold bioluminescent signal increase in female FVB-luc+ mice with lipopolysaccharide-induced acute inflammation, which is 30% attenuated by co-treatment with ascorbic acid and a pan-caspase inhibitor^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

741.81

C₃₂H₄₇N₅O₁₃S

1476735-95-1

Cbz-Ile-{Glu(Me)}-Thr-{Asp(Me)}-{d-Cys}

Cbz-I-{Glu(Me)}-T-{Asp(Me)}-{d-Cys}

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Van de Bittner GC, et al. Strategy for dual-analyte luciferin imaging: in vivo bioluminescence detection of hydrogen peroxide and caspase activity in a murine model of acute inflammation. J Am Chem Soc. 2013;135(5):1783-1795.  [Content Brief]