2. Others Anti-infection
  3. Fluorescent Dye HCV Dengue Virus
  4. Primulin

Primulin is a versatile fluorescent dye and bioactive compound widely used in analytical, biological, botanical and virological studies. Primulin acts as a versatile stain that labels plant cell walls and differentiates live and dead spermatozoa via distinct fluorescence patterns. Primulin exhibits strong albumin‑binding capacity. Primulin acts as a retrograde axonal tracer in neurobiological investigations. Primulin and its derivatives inhibit HCV NS3, block dengue virus NS3-mediated ATP hydrolysis, and disrupt HCV replicase assembly.

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Primulin

Primulin Chemical Structure

CAS No. : 8064-60-6

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Primulin Related Antibodies

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Description

Primulin is a versatile fluorescent dye and bioactive compound widely used in analytical, biological, botanical and virological studies. Primulin acts as a versatile stain that labels plant cell walls and differentiates live and dead spermatozoa via distinct fluorescence patterns. Primulin exhibits strong albumin‑binding capacity. Primulin acts as a retrograde axonal tracer in neurobiological investigations. Primulin and its derivatives inhibit HCV NS3, block dengue virus NS3-mediated ATP hydrolysis, and disrupt HCV replicase assembly[1][2][3].

In Vitro

Primulin is a versatile fluorescent stain that labels plant cell walls[1][2][3].
Primulin (10 μM, 72 h) neither reduces HCV replication complex numbers nor inhibits cellular NS3 protease activity in Huh7.5/HCV Con1sg Rluc replicon cells[1].
Primulin is an acid thiazole dye with stable fluorescence that binds to bovine albumin, is excluded by intact dorsal root ganglion neurons, and diffuses into neurons after freezing and thawing[2].
Primulin binds non‑covalently to lipids and enables phospholipid visualization on TLC plates without interfering with mass spectrometry analysis[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Primulin Related Antibodies

Immunofluorescence[1]

Cell Line: Huh7.5/HCV Con1sg Rluc replicon stable cells
Concentration: 10 μM
Incubation Time: 72 h
Result: Did not influence the number of HCV replication complexes.
In Vivo

Primulin (2-20%; intramuscular into vibrissae muscles; single dose) shows concentration- and time-dependent fluorescence enhancement, and 5% primulin labels nearly all facial neurons without causing motor endplate damage[2].
Primulin (5%; intramuscular into vibrissae muscles; single dose; bilateral) accumulation in regenerating facial neurons is significantly increased by ~200% of control levels at 11 days post-crush during mouse facial nerve regeneration[2].
Primulin (5-10%; bilateral intramuscular injection into vibrissae muscles)reduces accumulation in facial neurons after botulinum toxin paralysis. Low-dose tetanus toxin increases primulin accumulation, while high-dose decreases it by 17%[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss albino mice (2-month-old, unspecified gender)[2]
Dosage: 2%, 5%, 10%, 20% (50 μl)
Administration: intramuscular into vibrissae muscles; single dose
Result: Labeled approximately 30% of facial neurons with 2% primuline.
Labeled nearly all facial neurons with 5% primuline.
Enhanced fluorescence intensity in facial neurons in a concentration‑dependent manner.
Elevated fluorescence intensity in facial neurons in a time‑dependent manner after 5% primuline injection.
Produced no ultrastructural alterations in motor endplates at 5-20% concentrations.
Animal Model: Swiss albino mice (2-month-old, unspecified gender, facial nerve crushed at exit from stylomastoid foramen)[2]
Dosage: 5% (50 μl)
Administration: intramuscular into vibrissae muscles; single dose; bilateral
Result: Blocked primulin transport to facial neurons immediately and 3 days after nerve crush.
Increased primulin accumulation on the crushed side to ~200% of controls at 11 days.
Elevated primulin accumulation to ~135% and ~125% of controls at 18 and 34 days.
Showed no significant difference in primulin accumulation between groups at 92 days.
Animal Model: Swiss albino mice (2-month-old, unspecified gender, botulinum toxin type A injected into left vibrissae muscles)[2]
Dosage: 5%, 10% (5 μl)
Administration: intramuscular into vibrissae muscles; single dose; bilateral
Result: Reduced primulin fluorescence intensity by 7-19% on the toxin‑treated side in mice with ipsilateral vibrissae paralysis.
Showed no significant difference in primulin fluorescence intensity between both sides in mice with bilateral paralysis.
Animal Model: Swiss albino mice (2-month-old, unspecified gender, tetanus toxin injected into left vibrissae muscles)[2]
Dosage: 5%, 10% (5 μl)
Administration: intramuscular into vibrissae muscles; single dose; bilateral
Result: Increased primulin fluorescence intensity by 11-23% on the treated side in mice with low‑dose tetanus toxin.
Reduced primulin fluorescence intensity by 17% on the treated side in mice with high‑dose tetanus toxin‑induced paralysis.
Molecular Weight

475.54

Formula

C21H14N3NaO3S3
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

O=S(C1=C(C)C=CC2=C1SC(C3=CC(SC(C4=CC=C(N)C=C4)=N5)=C5C=C3)=N2)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Purity & Documentation
References
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Primulin Related Classifications

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× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Primulin8064-60-6PrimulineFluorescent DyeHCVDengue VirusHepatitis C virusDENVspermatozoaHuh7.5/HCV Con1sg Rluc replicon stable cellsHCV subgenomic repliconsalbuminHCV NS3mouse facial neuronsdengue virus NS3 helicaseHCV NS3 helicasedorsal root ganglion neuronsHCV NS3 proteaseInhibitorinhibitorinhibit

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