cell-isolation

MCE Streptavidin Magnetic Beads provide a fast and convenient method for numerous applications, including purification of proteins and nucleic acids, protein interaction studies, immunoprecipitation, immunoassays, pull-down and cell isolation. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Mouse CD8⁺ Cells Positive Selection Kit is designed for the isolation of CD8⁺ cells from single cell suspensions of mouse spleen cells, lymph nodes or other tissues.

MCE Mouse CD3⁺ T Cells Negative Selection Kit is designed for the isolation of CD3⁺ T cells from single cell suspensions of mouse spleen cells and lymph nodes.

MCE Human CD3⁺ T Cells Negative Selection Kit is designed for the isolation of CD3⁺ T cells from human peripheral blood mononuclear cells (PBMC).

MCE Mouse CD4⁺ T Cells Negative Selection Kit is designed for the isolation of CD4⁺ T cells from single cell suspensions of mouse spleen cells and lymph nodes.

MCE Human CD66b⁺ Cell Positive Selection Kit is designed for the efficient isolation of CD66b⁺ cells from human peripheral blood single-cell suspensions. CD66b⁺ cells are first specifically labeled with the CD66b Capture Antibody and subsequently captured using Releasable Magnetic Beads. Following magnetic separation, the beads are detached from the cell surface using Magnetic Beads Release Buffer, resulting in highly purified CD66b⁺ cells free of residual antibody and magnetic bead labeling.

MCE Human Neutrophil Negative Selection Kit can isolate neutrophils from fresh peripheral blood. The principle involves using biotin-labeled monoclonal antibodies to label non-target cells, followed by the removal of these non-target cells through streptavidin-labeled magnetic beads, achieving the selective isolation of human neutrophils.

MCE Mouse CD4⁺ CD25⁺ Regulatory T Cell Positive Selection Kit is designed for the isolation of CD4⁺ CD25⁺ regulatory T cells (Tregs) from mouse splenic single-cell suspensions. The kit utilizes a two-step isolation strategy. First, CD4 Enrichment Beads are used to deplete non-target cells and enrich CD4⁺ T cells. Subsequently, CD25 Capture Antibody specifically binds to CD25⁺ cells, which are then captured by Releasable Magnetic Beads. Following isolation, the magnetic beads are gently detached from the cell surface using Magnetic Beads Release Buffer, yielding label-free CD4⁺ CD25⁺ regulatory T cells with no residual antibodies or magnetic beads.

MCE Mouse Pan B Cell Negative Selection Kit enables the efficient isolation of total B cells from mouse spleen, lymph nodes, bone marrow, or peritoneal lavage fluid. The principle involves using biotin-labeled monoclonal antibodies to label non-target cells (non-B cells), followed by the removal of these non-target cells through streptavidin-labeled magnetic beads, achieving the selective isolation of mouse B Cells.

MCE Human CD3 Cell Depletion Magnetic Beads (Positive Selection) are magnetic beads covalently coupled with anti-human CD3 antibodies, enabling the specific binding and magnetic removal of CD3⁺ cells from single-cell suspensions derived from human peripheral blood mononuclear cells (PBMCs) or human peripheral blood leukocytes (WBCs). This allows efficient enrichment of the non-CD3⁺ cell population.

MCE Mouse CD3 Cell Depletion Magnetic Beads (Positive Selection) are magnetic beads covalently coupled with anti-mouse CD3 antibodies, enabling the specific binding and magnetic removal of CD3+ cells from single-cell suspensions derived from mouse spleen and other tissues. This allows efficient enrichment of the non-CD3⁺ cell population.

MCE Mouse Mature B Cell Negative Selection Kit enables the efficient isolation of mature B cells from single-cell suspensions prepared from mouse spleen, lymph nodes, or bone marrow. The principle involves using biotin-labeled monoclonal antibodies to label non-target cells (non-B cells), followed by the removal of these non-target cells through streptavidin-labeled magnetic beads, achieving the selective isolation of mouse mature B cells.

MCE Human CD34⁺ Cells Negative Selection Kit is designed for the isolation of CD34⁺ cells from human cord blood mononuclear cells (CBMC) or human peripheral blood mononuclear cells (PBMC).

MCE Mouse CD4⁺ Cells Positive Selection Kit is designed for the isolation of CD4⁺ cells from single cell suspensions of mouse spleen cells, lymph nodes or other tissues.

MCE Mouse CD8⁺ T Cells Negative Selection Kit is designed for the isolation of CD8⁺ T cells from single cell suspensions of mouse spleen cells and lymph nodes.