Mouse Pan B Cell Negative Selection Kit
MCE Mouse Pan B Cell Negative Selection Kit enables the efficient isolation of total B cells from mouse spleen, lymph nodes, bone marrow, or peritoneal lavage fluid. The principle involves using biotin-labeled monoclonal antibodies to label non-target cells (non-B cells), followed by the removal of these non-target cells through streptavidin-labeled magnetic beads, achieving the selective isolation of mouse B Cells.
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Storage :
4°C, 2 years
Do not freeze the magnetic beads
- Manual (3630KB)
- COA
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE Mouse Pan B Cell Negative Selection Kit enables the efficient isolation of total B cells from mouse spleen, lymph nodes, bone marrow, or peritoneal lavage fluid. The principle involves using biotin-labeled monoclonal antibodies to label non-target cells (non-B cells), followed by the removal of these non-target cells through streptavidin-labeled magnetic beads, achieving the selective isolation of mouse B Cells.
Features of MCE Mouse Pan B Cell Negative Selection Kit:
Simple and Rapid: No separation columns are required. Cell isolation can be completed using a magnetic separator.
High Purity: Purity of isolated mouse Pan B cells can reach up to 90%.
High Cell Viability: The isolated Pan B cells are free of antibody and magnetic bead labeling, maintaining high viability and normal cellular functions for downstream applications.
Storage
4°C, 2 years
Do not freeze the magnetic beads
Attention
1. Use low-retention pipette tips and tubes whenever possible to minimize bead and antibody loss due to surface adsorption.
2. Avoid high-speed centrifugation, drying, or freezing of the magnetic beads during storage and handling.
3. Do not leave the magnetic beads in a magnetic field for extended periods, as this may cause bead aggregation and reduced binding activity.
4. Thoroughly resuspend the beads before use. Handle gently throughout the procedure and avoid generating excessive bubbles.
5. This product must be used with a compatible magnetic separator.
6. Perform all procedures gently to minimize mechanical stress and maintain cell viability.
7. For optimal separation performance, use freshly prepared single-cell suspensions free of excessive cell clumps. If necessary, filter samples through a 70 μm cell strainer before separation.
8. This product is for R&D use only, not for drug, household, or other uses.
9. For your safety and health, please wear a lab coat and disposable gloves to operate. /p>
Components
| Components | HY-K0361-0.2 mL (For 1 × 10<sup>8</sup> cells) | HY-K0361-1 mL (For 5 × 10<sup>8</sup> cells) | HY-K0361-2 mL (For 1 × 10<sup>9</sup> cells) |
|---|---|---|---|
| Biotin-Antibody Mix | 20 μL | 100 μL | 200 μL |
| Streptavidin Magnetic Beads | 0.2 mL | 1 mL | 1 mL × 2 |
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