Anti-inflammation Effects of Oxysophoridine on Cerebral Ischemia-Reperfusion Injury in Mice

  • Inflammation. 2015 Dec;38(6):2259-68. doi: 10.1007/s10753-015-0211-4.
Yong-Sheng Wang  1 ,  Yu-Xiang Li  2 ,  Peng Zhao  1 ,  Hong-Bo Wang  1 ,  Ru Zhou  1 ,  Yin-Ju Hao  3 ,  Jie Wang  4 ,  Shu-Jing Wang  4 ,  Juan Du  1 ,  Lin Ma  5 ,  Tao Sun  5 ,  Jian-Qiang Yu  6  7  8
Affiliations
  • 1. Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China.
  • 2. College of Nursing, Ningxia Medical University, Yinchuan, 750004, China.
  • 3. Ningxia Medical University, Yinchuan, 750004, China.
  • 4. Medical Sci-Tech Research Center, Ningxia Medical University, Yinchuan, 750004, China.
  • 5. Ningxia Key Lab of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
  • 6. Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China. [email protected].
  • 7. Ningxia Hui Medicines Collaborative Innovation Center, Yinchuan, 750004, China. [email protected].
  • 8. Department of Pharmacology, Ningxia Medical University and Ningxia Hui Medicines Collaborative Innovation Center, Yinchuan, Ningxia, China. [email protected].
PMID: 26178478 DOI: 10.1007/s10753-015-0211-4
Abstract

Oxysophoridine (OSR) is a bioactive alkaloid extracted from the Sophora alopecuroides Linn. Our aim is to explore the potential anti-inflammation mechanism of OSR in cerebral ischemic injury. Mice were intraperitoneally pretreated with OSR (62.5, 125, and 250 mg/kg) or nimodipine (Nim) (6 mg/kg) for 7 days followed by cerebral ischemia. The inflammatory-related cytokines in cerebral ischemic hemisphere tissue were determined by immunohistochemistry staining, Western blot and enzyme-like immunosorbent assay (ELISA). OSR-treated groups observably suppressed the nuclear factor kappa B (NF-κB), intercellular adhesion molecule-1 (ICAM-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). OSR-treated group (250 mg/kg) markedly reduced the inflammatory-related protein prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8). Meanwhile, it dramatically increased the interleukin-10 (IL-10). Our study revealed that OSR protected neurons from ischemia-induced injury in mice by downregulating the proinflammatory cytokines and blocking the NF-κB pathway.

Keywords
NF-κB; anti-inflammation; cerebral ischemia; oxysophoridine.
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