In Vitro Cytotoxic Effects of Celecoxib, Mefenamic Acid, Aspirin and Indometacin on Several Cells Lines

  • J Dent (Shiraz). 2016 Sep;17(3):219-25.
Maryam Alsadat Hashemipour  1 ,  Hoda Mehrabizadeh Honarmand  2 ,  Farideh Falsafi  3 ,  Mehrnaz Tahmasebi Arashlo  3 ,  Saied Rajabalian  4 ,  Sayed Amir Hossein Gandjalikhan Nassab  5
Affiliations
  • 1. Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.
  • 2. Resident of Oral and Maxillofacial Surgery and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.
  • 3. Dentist, Dental and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.
  • 4. Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
  • 5. Medical Student, Kerman Dental and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.
PMID: 27602398
Abstract

Statement of the problem: Use of cyclooxygenase inhibitors as chemotherapy agents has attracted the attention of a large number of investigators in recent years. Given the importance of Cancer therapy, only a limited number of studies have been carried out to investigate the effects of cyclooxygenase inhibitors on specific cell lines.

Purpose: This research aimed to determine the in vitro cytotoxic effects of cyclooxygenase inhibitors (COX-1 and COX-2 inhibitors) on KB, Saos-2, 1321N, U-87MG, SFBF-PI 39 cell lines.

Materials and method: Powders of celecoxib, mefenamic acid, aspirin and indometacin were dissolved in the appropriate solvent. The viability of cell lines was carried out by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay technique. Data gathered from four separate experiments were expressed as mean±SD. Statistical significance was defined at p< 0.05 by using analysis of variance. Significant treatment mean values were subjected to post-hoc Tukey's test.

Results: Celecoxib showed marked cytotoxic effects on KB, Saos-2, and 1321N cells, which was significant in comparison with the control group. Celecoxib was not effective in killing U-87MG cell line. Mefenamic acid exerted cytotoxic effects on KB, Saos-2, and 1321N cells, where the viability was approximately 75%. U-87MG cells were resistant to mefenamic acid. Indometacin had the highest rate of activity on U-87MG cells, which was significant in comparison with the control group. Aspirin did not exhibit any activity on these cell lines and was not effective in killing U-87MG, KB, Saos-2, and 1321N cells.

Conclusion: This research showed that celecoxib, indometacin, and mefenamic acid have the cytotoxic effects on KB, Saos-2, 1321N and U-87MG cell lines. Therefore, it appears that these drugs can be considered as anti-neoplastic agents in the experimental phase.

Keywords
Aspirin; Cell lines; Cytotoxicity; Drug, Celecoxib; In Vitro; Indometacin; Mefenamic Acid.
Products