Camellia sinensis polysaccharide attenuates inflammatory responses via the ROS-mediated pathway by endocytosis
- Int J Biol Macromol. 2024 May;267(Pt 2):131674. doi: 10.1016/j.ijbiomac.2024.131674.
- 1. The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Science, Anqing Normal University, Anqing 246011, China.
- 2. The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Science, Anqing Normal University, Anqing 246011, China. Electronic address: [email protected].
- 3. The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Science, Anqing Normal University, Anqing 246011, China. Electronic address: [email protected].
Polysaccharide CSTPs extracted from Camellia sinensis tea-leaves possessed unique against oxidative damage by scavenging ROS. Herein, acid tea polysaccharide CSTPs-2 with tightly packed molecular structure was isolated, purified and characterized in this research. Furthermore, the effects of CSTPs-2 on ROS-involved inflammatory responses and its underlying mechanisms were investigated. The results suggest that CSTPs-2 dramatically reduced the inflammatory cytokines overexpression and LPS-stimulated cell damage. CSTPs-2 could trigger the dephosphorylation of downstream Akt/MAPK/NF-κB signaling proteins and inhibit nuclear transfer of p-NF-κB to regulate the synthesis and release of inflammatory mediators in LPS-stimulated cells by ROS scavenging. Importantly, the impact of CSTPs-2 in downregulating pro-inflammatory cytokines and mitigating ROS overproduction is associated with clathrin- or caveolae-mediated endocytosis uptake mechanisms, rather than TLR-4 receptor-mediated endocytosis. This study presents a novel perspective for investigating the cellular uptake mechanism of Polysaccharides in the context of anti-inflammatory mechanisms.