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KGF/FGF-7 Protein, Mouse

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Based on 1 Customer Validation

FGF-7 Protein, Mouse is a potent mitogen that enhances cell proliferation in various organs, including the skin, intestine, breast, liver, and lung.

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  • Species: Mouse
  • Source: E. coli
  • 保管条件:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for KGF/FGF-7 Protein, Mouse
  • Experimental Validation Results for KGF/FGF-7 Protein, Mouse
  • KGF/FGF-7 Protein, Mouse Product Image
  • Experimental Validation Results for KGF/FGF-7 Protein, Mouse
  • Experimental Validation Results for KGF/FGF-7 Protein, Mouse
  • KGF/FGF-7 Protein, Mouse Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
最寄りの代理店をお探しください Your local distributor: Hycultec GmbHHycultec
  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • 参考文献
  • Help & FAQs
Top Publications Citing

Use of MCE Products

生物活性

製品説明

FGF-7 Protein, Mouse is a potent mitogen that enhances cell proliferation in various organs, including the skin, intestine, breast, liver, and lung.

Background

Fibroblast growth factor-7 (FGF-7) is a potent mitogen that enhances cell proliferation in various organs, including the skin, intestine, breast, liver, and lung[1]. FGF-7, also known as keratinocyte growth factor, insofar as it displays a unique cell specificity. FGF7 binds to perlecan protein core and that exogenous perlecan efficiently reconstitutes FGF7 mitogenic activity in perlecan-deficient cells[2].

Verified Bioactivity

The ED50 is ≤10 ng/mL as measured by 4MBr-5 cells, corresponding to a specific activity of ≥1 × 105 units/mg.

Results
  • Experimental Validation Results for KGF/FGF-7 Protein, Mouse
    Measured in a cell proliferation assay using 4MBr 5 rhesus monkey epithelial cells. The ED50 for this effect is 8.477 ng/mL, corresponding to a specific activity is 1.18×105 units/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag Tag Free
  • アクセッション番号

    P36363 (C32-T194)

  • 遺伝子ID
    14178  [NCBI]
  • Molecular Construction
    • N-term
    • FGF-7 (C32-T194)
      Accession # P36363
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • 別名

    rMuFGF-7; HBGF-7; KGF

  • AA Sequence

    CNDMSPEQTATSVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNSYNIMEIRTVAVGIVAIKGVESEYYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHSGGEMFVALNQKGIPVKGKKTKKEQKTAHFLPMAIT

  • Predicted Molecular Mass

    18.7 kDa

  • 分子量

    Approximately 19 kDa, based on SDS-PAGE under reducing conditions.

  • 純度

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for KGF/FGF-7 Protein, Mouse
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

性状

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

輸送条件

Room temperature in continental US; may vary elsewhere.

参考文献

[1]. Tichelaar JW, et al. Conditional expression of fibroblast growth factor-7 in the developing and mature lung. J Biol Chem. 2000 Apr 21;275(16):11858-64. [Content Brief]

[2]. Mongiat M, et al. The protein core of the proteoglycan perlecan binds specifically to fibroblast growth factor-7. J Biol Chem. 2000 Mar 10;275(10):7095-100.

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)
=
Mass (in vial)
÷
Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始)
×
体積 (開始)
=
濃度 (終了)
×
体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50)
106 ÷
ng/mL

KGF/FGF-7 Protein, Mouse 関連分類

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • Measured in a cell proliferation assay using 4MBr 5 rhesus monkey epithelial cells. The ED50 for this effect is 8.477 ng/mL, corresponding to a specific activity is 1.18×105 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using 4MBr 5 rhesus monkey epithelial cells. The ED50 for this effect is 8.477 ng/mL, corresponding to a specific activity is 1.18×105 units/mg.

  • KGF/FGF-7 Protein, Mouse Product Image
    1/16
  • Experimental Validation Results for KGF/FGF-7 Protein, Mouse
  • Experimental Validation Results for KGF/FGF-7 Protein, Mouse
  • KGF/FGF-7 Protein, Mouse Product Image