Nectin-4 Protein, Human (HEK293, His)

The Nectin-4 Protein IgV domain is pivotal in cell adhesion, participating in trans-homophilic and -heterophilic interactions, notably with NECTIN1. While it doesn't serve as an alpha-herpesvirus entry receptor, it functions as a receptor for measles virus, enabling virus entry into host cells. This dual role underscores the importance of Nectin-4 in cell adhesion and its specific role as a measles virus infection receptor. Nectin-4 Protein, Human (HEK293, His) is the recombinant human-derived Nectin-4 protein, expressed by HEK293 , with C-6*His labeled tag.

The Nectin-4 Protein IgV domain plays a crucial role in cell adhesion, engaging in both trans-homophilic and -heterophilic interactions. Specifically, it forms interactions with NECTIN1, contributing to cellular adhesion processes. However, it does not function as a receptor for alpha-herpesvirus entry into cells. In the context of microbial infection, the Nectin-4 IgV domain acts as a receptor for measles virus, facilitating the entry of the virus into host cells. This highlights the dual role of Nectin-4 in cell adhesion and its significance as a specific receptor for measles virus infection.

1.Immobilized Anti-Human Nectin-4 mAb-mFc at 2 μg/mL (100 μl/well) can bind Human Nectin-4-His .The ED₅₀ of Human Nectin-4-His is 16.29-50 ng/mL.
2.Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 2.94 μg/mL, corresponding to a specific activity is 3.4×10⁵ units/mg.
3.Loaded Bulumtatug (HY-P990977) on AHC2 biosensor, can bind Nectin-4 Protein, Human (HEK293, His) with an affinity constant of 4.327E-09 M as determined in BLI assay.
4.Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-4, His Tag at 1 μg/mL (100 μL/well) can bind Enfortumab with a linear range of 0.06-2 ng/mL.

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  Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 2.94 μg/mL, corresponding to a specific activity is 3.4×10⁵ units/mg.
• 
  Loaded Bulumtatug (HY-P990977) on AHC2 biosensor, can bind Nectin-4 Protein, Human (HEK293, His) with an affinity constant of 4.327E-09 M as determined in BLI assay.

Human

HEK293

C-6*His

Q96NY8-1 (G32-V351)

81607  [NCBI]

Partial

GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVV

Approximately 40-48 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 8% sucrose, 0.05% Tween 80, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.