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Nectin-4 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

The Nectin-4 Protein IgV domain is pivotal in cell adhesion, participating in trans-homophilic and -heterophilic interactions, notably with NECTIN1. While it doesn't serve as an alpha-herpesvirus entry receptor, it functions as a receptor for measles virus, enabling virus entry into host cells. This dual role underscores the importance of Nectin-4 in cell adhesion and its specific role as a measles virus infection receptor. Nectin-4 Protein, Human (HEK293, His) is the recombinant human-derived Nectin-4 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Nectin-4 Protein, Human (HEK293, His)
  • Experimental Validation Results for Nectin-4 Protein, Human (HEK293, His)
  • Experimental Validation Results for Nectin-4 Protein, Human (HEK293, His)
  • Nectin-4 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The Nectin-4 Protein IgV domain is pivotal in cell adhesion, participating in trans-homophilic and -heterophilic interactions, notably with NECTIN1. While it doesn't serve as an alpha-herpesvirus entry receptor, it functions as a receptor for measles virus, enabling virus entry into host cells. This dual role underscores the importance of Nectin-4 in cell adhesion and its specific role as a measles virus infection receptor. Nectin-4 Protein, Human (HEK293, His) is the recombinant human-derived Nectin-4 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The Nectin-4 Protein IgV domain plays a crucial role in cell adhesion, engaging in both trans-homophilic and -heterophilic interactions. Specifically, it forms interactions with NECTIN1, contributing to cellular adhesion processes. However, it does not function as a receptor for alpha-herpesvirus entry into cells. In the context of microbial infection, the Nectin-4 IgV domain acts as a receptor for measles virus, facilitating the entry of the virus into host cells. This highlights the dual role of Nectin-4 in cell adhesion and its significance as a specific receptor for measles virus infection.

Verified Bioactivity

1.Immobilized Anti-Human Nectin-4 mAb-mFc at 2 μg/mL (100 μl/well) can bind Human Nectin-4-His .The ED50 of Human Nectin-4-His is 16.29-50 ng/mL.
2.Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2.94 μg/mL, corresponding to a specific activity is 3.4×105 units/mg.
3.Loaded Bulumtatug (HY-P990977) on AHC2 biosensor, can bind Nectin-4 Protein, Human (HEK293, His) with an affinity constant of 4.327E-09 M as determined in BLI assay.
4.Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-4, His Tag at 1 μg/mL (100 μL/well) can bind Enfortumab with a linear range of 0.06-2 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q96NY8-1 (G32-V351)

  • Gene ID
    81607  [NCBI]
  • Molecular Construction
    • N-term
    • Nectin-4 (G32-V351)
      Accession # Q96NY8-1
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NECTIN4; Poliovirus Receptor-Related Protein 4; Prev. PVRL4; Poliovirus Receptor-Related 4; Nectin-4; Nectin 4; PRR4; EDSS1; LNIR; Nectin Cell Adhesion Molecule 4; Ig Superfamily Receptor LNIR

  • AA Sequence

    GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVV

  • Molecular Weight

    Approximately 40-47 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 8% sucrose, 0.05% Tween 80, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2.94 μg/mL, corresponding to a specific activity is 3.4×105 units/mg.

  • 1/16

    MedChemExpress Validation
     Loaded Bulumtatug (HY-P990977) on AHC2 biosensor, can bind Nectin-4 Protein, Human (HEK293, His) with an affinity constant of 4.327E-09 M as determined in BLI assay.

  • Nectin-4 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Nectin-4 Protein, Human (HEK293, His)
  • Experimental Validation Results for Nectin-4 Protein, Human (HEK293, His)
  • Experimental Validation Results for Nectin-4 Protein, Human (HEK293, His)
  • Nectin-4 Protein, Human (HEK293, His) Product Image