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Vinculin Protein, Human

Cat. No.: HY-P70994
COA Handling Instructions

Vinculin Protein is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. It regulates adhesion by directly binding to actin, stimulating actin polymerization and recruiting actin remodeling proteins. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex, may also play important roles in cell morphology and locomotion. Vinculin Protein, Human is the recombinant human-derived Vinculin protein, expressed by E. coli , with tag free. The total length of Vinculin Protein, Human is 1065 a.a., with molecular weight of ~115.0 kDa.

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Description

Vinculin Protein is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. It regulates adhesion by directly binding to actin, stimulating actin polymerization and recruiting actin remodeling proteins. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex, may also play important roles in cell morphology and locomotion. Vinculin Protein, Human is the recombinant human-derived Vinculin protein, expressed by E. coli , with tag free. The total length of Vinculin Protein, Human is 1065 a.a., with molecular weight of ~115.0 kDa.

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Vinculin has no enzymatic activity. It regulates adhesion by directly binding to actin, stimulating actin polymerization and recruiting actin remodeling proteins. In the absence of vinculin, cell-matrix and cell-cell adhesion are dramatically impaired, indicating vinculin plays a critical role in human physiology. Vinculin stabilizes focal adhesions and thereby suppresses cell migration, an effect that is relieved by transient changes in the local concentrations of inositol phospholipids. Vinculin provides a mechanical link, controls cell signalling processes, affects contractility and adhesion protein turnover. Vinculin is thought to function as a tumour suppressor by supporting anchorage-dependent cell growth and suppressing tumour metastasis by reducing cell motility. In addition, Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex, may also play important roles in cell morphology and locomotion[1][2][3][4].

Species

Human

Source

E. coli

Tag

Tag Free

Accession

AAH39174.1 (P2-Q1066)

Gene ID
Molecular Construction
N-term
Vinculin (P2-Q1066)
Accession # AAH39174.1
C-term
Synonyms
Vinculin; Metavinculin; VCL
AA Sequence

PVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTLEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Molecular Weight

Approximately 115.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Vinculin Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Vinculin Protein, Human
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HY-P70994
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