Vinculin Protein, Mouse (HEK293, His)

Vinculin Protein, a crucial actin filament (F-actin)-binding protein, actively engages in cell-matrix and cell-cell adhesion, regulating E-cadherin expression and enhancing mechanosensing with the E-cadherin complex for cell morphology and locomotion (By similarity). Vinculin self-associates and is part of a complex with THSD1, PTK2/FAK1, TLN1, and VCL (By similarity). Interactions with proteins like APBB1IP, NRAP, TLN1, SYNM, CTNNB1, SORBS1, and CTNNA1 serve specific cellular functions. The CTNNB1 interaction is crucial for vinculin's localization to cell-cell junctions and its role in regulating E-cadherin surface expression (By similarity). Additionally, vinculin binds to ACTN4, inducing conformational changes for functional versatility (By similarity). Vinculin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Vinculin protein, expressed by HEK293, with C-His labeled tag. The total length of Vinculin Protein, Mouse (HEK293, His) is 1066 a.a., with molecular weight of ~118 kDa.

Vinculin, a crucial actin filament (F-actin)-binding protein, actively participates in cell-matrix adhesion and cell-cell adhesion processes. It plays a regulatory role in the expression of cell-surface E-cadherin and enhances mechanosensing by the E-cadherin complex, contributing to cell morphology and locomotion (By similarity). Notably, vinculin exhibits self-association properties and is part of a complex that includes THSD1, PTK2/FAK1, TLN1, and VCL (By similarity). Its interactions extend to various proteins such as APBB1IP, NRAP, TLN1, SYNM, CTNNB1, SORBS1, and CTNNA1, each interaction serving specific cellular functions. The interaction with CTNNB1 is particularly essential for vinculin's localization to cell-cell junctions and its role in regulating the cell surface expression of E-cadherin (By similarity). Additionally, vinculin binds to ACTN4, triggering conformational changes that further contribute to its functional versatility (By similarity).

Measured by its binding ability in a functional ELISA .Immobilized Vinculin at 1 μg/ml can bind Anti-Vinculin antibody, the EC₅₀ of human Vinculin protein is 1.426 ng/mL, corresponding to a specific activity is 7.012×10⁵ units/mg.

Mouse

HEK293

C-His

Q64727 (M1-Q1066)

22330  [NCBI]

Approximately 118 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.