Vinculin Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Vinculin (Vinculin Protein) is an important actin filament (F-actin) binding protein, actively participates in cell-matrix and cell-cell adhesion, regulates E-cadherin expression, and regulates E-cadherin expression through E-cadherin.Complex enhances mechanosensing of cell morphology and movement (by similarity).Vinculin self-associates with and is part of the complex (by similarity) with THSD1, PTK2/FAK1, TLN1 and VCL.Vinculin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Vinculin protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Vinculin (Vinculin Protein) is an important actin filament (F-actin) binding protein, actively participates in cell-matrix and cell-cell adhesion, regulates E-cadherin expression, and regulates E-cadherin expression through E-cadherin.Complex enhances mechanosensing of cell morphology and movement (by similarity).Vinculin self-associates with and is part of the complex (by similarity) with THSD1, PTK2/FAK1, TLN1 and VCL.Vinculin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Vinculin protein, expressed by HEK293 , with C-His labeled tag.
Background
Vinculin, a crucial actin filament (F-actin)-binding protein, actively participates in cell-matrix adhesion and cell-cell adhesion processes. It plays a regulatory role in the expression of cell-surface E-cadherin and enhances mechanosensing by the E-cadherin complex, contributing to cell morphology and locomotion (By similarity). Notably, vinculin exhibits self-association properties and is part of a complex that includes THSD1, PTK2/FAK1, TLN1, and VCL (By similarity). Its interactions extend to various proteins such as APBB1IP, NRAP, TLN1, SYNM, CTNNB1, SORBS1, and CTNNA1, each interaction serving specific cellular functions. The interaction with CTNNB1 is particularly essential for vinculin's localization to cell-cell junctions and its role in regulating the cell surface expression of E-cadherin (By similarity). Additionally, vinculin binds to ACTN4, triggering conformational changes that further contribute to its functional versatility (By similarity).
Verified Bioactivity
Measured by its binding ability in a functional ELISA .Immobilized Vinculin at 1 μg/ml can bind Anti-Vinculin antibody, the EC50 of human Vinculin protein is 1.426 ng/mL, corresponding to a specific activity is 7.012×105 units/mg.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
Q64727 (M1-Q1066)
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Molecular Construction
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N-term
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Vinculin (M1-Q1066)
Accession # Q64727 -
His
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C-term
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Protein Length
Full Length
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Synonyms
VCL; Vinculin; Metavinculin; VINC; MV; Epididymis Secretory Sperm Binding Protein; Epididymis Luminal Protein 114; Meta-Vinculin; HEL114; CMD1W; CMH15; MVCL
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AA Sequence
MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ
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Predicted Molecular Mass
118.2 kDa
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Molecular Weight
Approximately 118 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (242 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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