Cat. No.: HY-N2068

Didymin

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Didymin, a flavonoid glycoside, possesses antioxidant and anticancer properties. Didymin induces apoptosis by inhibiting N-Myc and upregulating RKIP in neuroblastoma.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 14259-47-3
Formula: C₂₈H₃₄O₁₄
Molecular Weight:594.56

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Didymin Chemical Structure

CAS No.: 14259-47-3

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(In Japan)

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Didymin, a flavonoid glycoside, possesses antioxidant and anticancer properties. Didymin induces apoptosis by inhibiting N-Myc and upregulating RKIP in neuroblastoma^[1].

In Vitro

Didymin (1-20 μM; 48 h) inhibits cell proliferation in human non-small-cell lung cancer cells^[1].
Didymin (10-20 μM; 12-48 h) induced apoptosis in A549 and H460 cells, without affecting the cell cycle distribution.^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

A549 and H460 cells
Concentration:

1 μM, 5 μM, 10 μM, 20 μM
Incubation Time:

48 h
Result:

Inhibited cell proliferation in human non-small-cell lung cancer cells.

Apoptosis Analysis^[1]

Cell Line:

A549 and H460 cells
Concentration:

10 μM, 20 μM
Incubation Time:

12 h, 24 h, 48 h
Result:

Induced apoptosis in A549 and H460 cells, without affecting the cell cycle distribution.

In Vivo

Didymin (6 mg/kg/day; i.p.; daily for 28 days) delays the tumor growth in nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male nude mice [6 weeks old; BALB/cA-nu (nu/nu)] injected with A549 cells^[1]
Dosage:

6 mg/kg/day
Administration:

i.p.; daily for 28 days
Result:

Inhibited tumor growth in nude mice.

Chemical Information

CAS No. 14259-47-3
Appearance Solid
Molecular Weight 594.56
Formula C₂₈H₃₄O₁₄
Color White to yellow
SMILES

O=C1C[C@@H](C2=CC=C(OC)C=C2)OC3=CC(O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO[C@H]5[C@@H]([C@@H]([C@H]([C@H](C)O5)O)O)O)O4)O)O)O)=CC(O)=C13
Structure Classification
- Flavonoids
- Flavonones
- Phenols
- Monophenols
Initial Source
- Plants
- Rutaceae
- Citrus
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (5)

All Cell Imaging/StainingCell Proliferation/Viability AssayRT-PCRApoptosis AnalysisWB

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Journal Impact Factor

Nat Commun

RKIP regulates bone marrow macrophage differentiation to mediate osteoclastogenesis and H-type vessel formation. [Abstract]

2025 Aug 15;16(1):7604. PMID: 40817102

Didymin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 15;16(1):7604. [Abstract]

TRAP staining to detect osteoclastogenesis of PBMCs with various concentrations of Didymin (10, 20 μM) treatment.

Didymin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 15;16(1):7604. [Abstract]

The mRNA level of ACP5, CTSK, NFATc1 in PBMCs with vehicle or Didymin (10, 20 μM) treatment in the presence or absence of h-RANKL for 10 days.

Didymin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 15;16(1):7604. [Abstract]

Western blots of RKIP, c-Fos, and NFATc1 protein levels in PBMCs with vehicle or Didymin (10, 20 μM) treatment in the presence or absence of h-RANKL for 10 days.
J Transl Med

Didymin alleviates metabolic dysfunction-associated fatty liver disease (MAFLD) via the stimulation of Sirt1-mediated lipophagy and mitochondrial biogenesis. [Abstract]

2023 Dec 19;21(1):921. PMID: 38115075

Didymin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Dec 19;21(1):921. [Abstract]

Cell viability of AML12 cells treated with PA and different concentrations of Didymin. (10, 20, 50 μM).

Didymin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Dec 19;21(1):921. [Abstract]

Co-treatment of Didymin (50 μM) and EX-527 resulted in a re-increase in the number of apoptotic cells by Flow cytometry analysis.
J Ethnopharmacol

Didymin alleviates neuropathic pain by targeting RKIP-mediated NF-κB/NLRP3 crosstalk to inhibit pyroptosis and neuroinflammation. [Abstract]

2026 Mar 25:359:121046. PMID: 41453551
Diabetol Metab Syndr

Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance. [Abstract]

2024 Jan 3;16(1):7. PMID: 38172956
Yonsei Med J

Didymin Alleviates Cerebral Ischemia-Reperfusion Injury by Activating the PPAR Signaling Pathway. [Abstract]

2022 Oct;63(10):956-965. PMID: 36168249

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (420.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6819 mL

8.4096 mL

16.8192 mL

5 mM

0.3364 mL

1.6819 mL

3.3638 mL

10 mM

0.1682 mL

0.8410 mL

1.6819 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.17 mg/mL (3.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.17 mg/mL (3.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.17 mg/mL (3.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hung JY, et al. Didymin, a dietary flavonoid glycoside from citrus fruits, induces Fas-mediated apoptotic pathway in human non-small-cell lung cancer cells in vitro and in vivo. Lung Cancer. 2010 Jun;68(3):366-74. [Content Brief]

[2]. Singhal J, et al. Didymin induces apoptosis by inhibiting N-Myc and upregulating RKIP in neuroblastoma. Cancer Prev Res (Phila). 2012 Mar;5(3):473-83. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6819 mL	8.4096 mL	16.8192 mL	42.0479 mL
	5 mM	0.3364 mL	1.6819 mL	3.3638 mL	8.4096 mL
	10 mM	0.1682 mL	0.8410 mL	1.6819 mL	4.2048 mL
	15 mM	0.1121 mL	0.5606 mL	1.1213 mL	2.8032 mL
	20 mM	0.0841 mL	0.4205 mL	0.8410 mL	2.1024 mL
	25 mM	0.0673 mL	0.3364 mL	0.6728 mL	1.6819 mL
	30 mM	0.0561 mL	0.2803 mL	0.5606 mL	1.4016 mL
	40 mM	0.0420 mL	0.2102 mL	0.4205 mL	1.0512 mL
	50 mM	0.0336 mL	0.1682 mL	0.3364 mL	0.8410 mL
	60 mM	0.0280 mL	0.1402 mL	0.2803 mL	0.7008 mL
	80 mM	0.0210 mL	0.1051 mL	0.2102 mL	0.5256 mL
	100 mM	0.0168 mL	0.0841 mL	0.1682 mL	0.4205 mL

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Didymin

Apoptosis Compound Library

Natural Product Library

Anti-Cancer Compound Library

Antioxidant Compound Library

Glycoside Compound Library

Lipid Compound Library

Medicine Food Homology Compound Library

Phenols Library

Traditional Chinese Medicine Active Compound Library

Flavonoids Library

Anti-Lung Cancer Compound Library

Food-Sourced Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Cell Death Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

RNA Binding Bioactive Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Didymin

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Didymin Related Classifications

Cat. No.: HY-N2068