Stauprimide
Based on 1 Customer Validation
Stauprimide is a staurosporine analog that promotes embryonic stem cell (ESC) differentiation. Stauprimide is a non-broad spectrum inhibitor that binds to the MYC transcription factor NME2 and blocks its nuclear localization in ESCs, which results in down-regulation of MYC transcription.
For research use only. We do not sell to patients.
- Purity: 99.0%
- CAS No.: 154589-96-5
- Formula: C35H28N4O5
- Molecular Weight:584.62
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Stauprimide (10 μM; 6 hours) suppresses MYC transcription in the majority of cell lines tested with an EC50 range of 30 nM-8 μM, and decreases MYC levels between 15% to over 90%[1]. Stauprimide (2-8 μM; 24-72 hours) down-regulates MYC leads to the inhibition of cell proliferation in vitro with an IC50 of 780 nM in RXF 393 cells[1]. Stauprimide (5 μM; 3 hours) suppresses MYC Transcription by decreasing NME2 Nuclear Translocation[1]. Stauprimide (4-10 μM; 6 hours) acts with different EC50s and with different degrees of maximal MYC mRNA down-regulation in different cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Renal cancer cell line RXF 393 cells
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Concentration:2 μM, 4 μM, 8 μM
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Incubation Time:24 hours, 48 hours, 72 hours
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Result:Inhibited cell proliferation at concentrations of 2-8 μM.
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Cell Line:Renal cancer cell line RXF 393 cells and CAKI-1 cells
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Concentration:5 μM
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Incubation Time:3 hours
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Result:Decreased nuclear localized NME2.
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Cell Line:CA46 cells; Ramos cells; RXF393 cells; TK10 cells; KG1A cells; CAKI-1 cells
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Concentration:4μM, 6μM, 8μM, 10μM
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Incubation Time:6 hours
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Result:Suppressed MYC transcription in KG1A cells with an EC50 of 400±50 nM and a suppression of 90%; CA46 cells were resistant to stauprimide.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Xenograft tumor models with RXF 393 and CAKI-1 cells in NOD/SCID mice[1]
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Dosage:50 mg/kg
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Administration:Oral administration; 50 mg/kg; once daily; 30 days, 55 days
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Result:Blocked tumor growth in mice injected with either RXF 393 or CAKI-1 cells during the dosing periods.
Chemical Information
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CAS No. 154589-96-5
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Appearance Solid
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Molecular Weight 584.62
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Formula C35H28N4O5
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Color Light yellow to yellow
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SMILES
C[C@@]([C@@H]1OC)(O[C@](N2C3=CC=CC=C43)([H])C[C@H]1N(C)C(C5=CC=CC=C5)=O)N6C(C2=C4C(C7=O)=C8C(N7)=O)=C8C9=C6C=CC=C9
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 10 mg/mL (17.11 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7105 mL | 8.5526 mL | 17.1051 mL | 42.7628 mL |
| 5 mM | 0.3421 mL | 1.7105 mL | 3.4210 mL | 8.5526 mL | |
| 10 mM | 0.1711 mL | 0.8553 mL | 1.7105 mL | 4.2763 mL | |
| 15 mM | 0.1140 mL | 0.5702 mL | 1.1403 mL | 2.8509 mL |
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