4-IPP (Synonyms: 4-Iodo-6-phenylpyrimidine)

Name: 4-IPP
Brand: MedChemExpress
SKU: HY-110063
Rating: 4.5 (2 reviews)

Cat. No.: HY-110063 Purity: 99.46%: FAQs
Data Sheet SDS COA Handling Instructions

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4-IPP (4-Iodo-6-phenylpyrimidine) is a specific suicide substrate and irreversible inhibitor of macrophage migration inhibitory factor (MIF).

For research use only. We do not sell to patients.

4-IPP Chemical Structure

CAS No. : 41270-96-6

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 132	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 132	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE 4-IPP

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Description

4-IPP (4-Iodo-6-phenylpyrimidine) is a specific suicide substrate and irreversible inhibitor of macrophage migration inhibitory factor (MIF)^[1].

IC₅₀ & Target

MIF^[1]

In Vitro

4-IPP is a specific suicide substrate for MIF that binds covalently and irreversibly to MIF to inhibit its biologic activity^[1].
4-IPP inhibits RANKL-induced p65 phosphorylation and nuclear translocation by preventing the interaction of MIF with thioredoxin-interacting protein-p65 complexes^[1].
4-IPP can inhibit receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis and potentiate osteoblast-mediated mineralization and bone nodule formation^[1].
4-IPP (0.5-200μM;24-72 hours) inhibits osteoclastogenesis in a dose-dependent manner^[1].
4-IPP(5-20μM; 5 days) inhibits RANKL-induced osteoclast differentiation and bone resorption ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	BMMs
Concentration:	0.5 μM, 1 μM, 2.5 μM, 5 μM, 10 μM, 25 μM, 50 μM, 100 μM, 200 μM
Incubation Time:	24 hours, 72 hours
Result:	Inhibited osteoclastogenesis in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	BMMs
Concentration:	5 μM,10 μM, 20 μM
Incubation Time:	1 day, 3 days, 5 days
Result:	Inhibited RANKL-induced osteoclast differentiation and bone resorption.

In Vivo

4-IPP (1 mg/kg, 5 mg/kg; every 2 days; for 8 weeks) ameliorates the bone loss associated with estrogen deficiency by reducing osteoclastic activities and enhancing osteoblastic bone formation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-weeks-old C57BL/6J male mice^[1]
Dosage:	1 mg/kg, 5 mg/kg
Administration:	Intraperitoneal injection; every 2 days; for 8 weeks
Result:	Alleviated OVX-induced osteoporosis.

Molecular Weight

282.08

Formula

C₁₀H₇IN₂

CAS No.

41270-96-6

Appearance

Solid

Color

White to yellow

SMILES

IC1=CC(C2=CC=CC=C2)=NC=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (354.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5451 mL	17.7255 mL	35.4509 mL
5 mM	0.7090 mL	3.5451 mL	7.0902 mL
10 mM	0.3545 mL	1.7725 mL	3.5451 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.46%

Select Batch:

Data Sheet (278 KB) SDS (0 KB)

COA (248 KB) HNMR (227 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Zheng L, et al. Macrophage migration inhibitory factor (MIF) inhibitor 4-IPP suppresses osteoclast formation and promotes osteoblast differentiation through the inhibition of the NF-κB signaling pathway. FASEB J. 2019 Jun;33(6):7667-7683. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5451 mL	17.7255 mL	35.4509 mL	88.6273 mL
	5 mM	0.7090 mL	3.5451 mL	7.0902 mL	17.7255 mL
	10 mM	0.3545 mL	1.7725 mL	3.5451 mL	8.8627 mL
	15 mM	0.2363 mL	1.1817 mL	2.3634 mL	5.9085 mL
	20 mM	0.1773 mL	0.8863 mL	1.7725 mL	4.4314 mL
	25 mM	0.1418 mL	0.7090 mL	1.4180 mL	3.5451 mL
	30 mM	0.1182 mL	0.5908 mL	1.1817 mL	2.9542 mL
	40 mM	0.0886 mL	0.4431 mL	0.8863 mL	2.2157 mL
	50 mM	0.0709 mL	0.3545 mL	0.7090 mL	1.7725 mL
	60 mM	0.0591 mL	0.2954 mL	0.5908 mL	1.4771 mL
	80 mM	0.0443 mL	0.2216 mL	0.4431 mL	1.1078 mL
	100 mM	0.0355 mL	0.1773 mL	0.3545 mL	0.8863 mL

4-IPP Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-IPP41270-96-64-Iodo-6-phenylpyrimidineMacrophage migration inhibitory factor (MIF)MIFRANKLosteoclastogenesisosteoblastInhibitorinhibitorinhibit

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4-IPP (Synonyms: 4-Iodo-6-phenylpyrimidine)

Customer Review

4-IPP Related Antibodies

2 Publications Citing Use of MCE 4-IPP

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

4-IPP Related Antibodies

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Complete Stock Solution Preparation Table

4-IPP Related Classifications

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