8-pCPT-2'-O-Me-cAMP-AM
8-pCPT-2'-O-Me-cAMP-AM is a cyclic AMP analogue, selectively activates Epac-Rap signaling pathway. 8-pCPT-2'-O-Me-cAMP-AM protects renal function by activating Epac from ischemia injury. 8-pCPT-2'-O-Me-cAMP-AM also stimulates insulin secretion by interaction with PKA pathway.
For research use only. We do not sell to patients.
- CAS No.: 1152197-23-3
- Formula: C20H21ClN5O8PS
- Molecular Weight:557.90
-
Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
8-pCPT-2'-O-Me-cAMP-AM (2.5 μM; 30 min) activates Epac and prevents adherens junction disassembly during hypoxia (60 min)[1].
8-pCPT-2'-O-Me-cAMP-AM can cross the plasma membrane and is able to alter diverse cellular functions that include Rap1 GTPase activity, PKB, and ERK1/2 protein kinase activity, phospholipase C activity, Ca2+ signaling, ion channel activity, exocytosis, cell adhesion, and gene expression[2].
8-pCPT-2'-O-Me-cAMP-AM stimulates insulin secretion with dose-dependent and glucose metabolism-dependent (0.1 or 1.11 mM) actions[2].
8-pCPT-2'-O-Me-cAMP-AM (20 μM) activates the cAMP reporter Epac1-camps, while 8-pCPT-2'-O-Me-cAMP doesn’t in INS-1 cells[2].
8-pCPT-2'-O-Me-cAMP-AM (0.3-3.0 μM; 0-300 sec) activates Epac1-camps in a dose- and time-dependent manner in high throughput assay[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
8-pCPT-2'-O-Me-cAMP-AM preserves renal function by Epac activation and reduces tubular epithelial-cell stress during ischemia[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:IR injuried mouse model[1]
-
Dosage:1.45 mM
-
Administration:Intrarenal injection; mice were sacrificed at 24, 48, or 72 hours after ischemia
-
Result:Protected renal injury during ischemia.
Chemical Information
-
CAS No. 1152197-23-3
-
Appearance Solid-Liquid Mixture
-
Molecular Weight 557.90
-
Formula C20H21ClN5O8PS
-
Color Colorless to off-white
-
SMILES
NC1=C2C(N([C@@]3([H])[C@@H]([C@@]4([H])[C@](COP(OCOC(C)=O)(O4)=O)([H])O3)OC)C(SC5=CC=C(C=C5)Cl)=N2)=NC=N1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 55.79 mg/mL (100.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (279 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Stokman G, et al. Epac-Rap signaling reduces cellular stress and ischemia-induced kidney failure. J Am Soc Nephrol. 2011 May;22(5):859-72. [Content Brief]
[2]. Chepurny OG, et al. Enhanced Rap1 activation and insulin secretagogue properties of an acetoxymethyl ester of an Epac-selective cyclic AMP analog in rat INS-1 cells: studies with 8-pCPT-2'-O-Me-cAMP-AM. J Biol Chem. 2009 Apr 17;284(16):10728-36. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7924 mL | 8.9622 mL | 17.9244 mL | 44.8109 mL |
| 5 mM | 0.3585 mL | 1.7924 mL | 3.5849 mL | 8.9622 mL | |
| 10 mM | 0.1792 mL | 0.8962 mL | 1.7924 mL | 4.4811 mL | |
| 15 mM | 0.1195 mL | 0.5975 mL | 1.1950 mL | 2.9874 mL | |
| 20 mM | 0.0896 mL | 0.4481 mL | 0.8962 mL | 2.2405 mL | |
| 25 mM | 0.0717 mL | 0.3585 mL | 0.7170 mL | 1.7924 mL | |
| 30 mM | 0.0597 mL | 0.2987 mL | 0.5975 mL | 1.4937 mL | |
| 40 mM | 0.0448 mL | 0.2241 mL | 0.4481 mL | 1.1203 mL | |
| 50 mM | 0.0358 mL | 0.1792 mL | 0.3585 mL | 0.8962 mL | |
| 60 mM | 0.0299 mL | 0.1494 mL | 0.2987 mL | 0.7468 mL | |
| 80 mM | 0.0224 mL | 0.1120 mL | 0.2241 mL | 0.5601 mL |
8-pCPT-2'-O-Me-cAMP-AM Related Classifications
HY-107544 Related Classifications
Powered by Bioz