AB-680

Name: AB-680
Brand: MedChemExpress
SKU: HY-125286
Rating: 5.0 (22 reviews)

Cat. No.: HY-125286 Purity: 99.71% ee.: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

AB-680 is a highly potent, reversible and selective inhibitor of CD73 (an ecto-nucleotidase), with a K_i of 4.9 pM for hCD73, displays >10,000-fold selectivity over related ecto-nucleotidases CD39. Anti-tumor activity.

For research use only. We do not sell to patients.

CAS No. : 2105904-82-1

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of AB-680:

AB-680 ammonium Get quote

22 Publications Citing Use of MCE AB-680

ELISA

Bio/Physico-chemical Assay

In Vivo Efficacy Study

Histological Imaging/Staining

IHC

: AB-680 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Feb 17;85(4):692-703. [Abstract]; IFNγ levels measured by ELISA in supernatant of NY-ESO/624mel cultures exposed to AMP (300 μmol/L) with or without AB-680 (0.5 μmol/L) for 24 hous.

: AB-680 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Feb 17;85(4):692-703. [Abstract]; Percentage of labeled (M + 5) ADO and AMP out of total intracellular adenosine (ADO) in activated (1 ng/mL αCD3) NY-ESO-1–specific T cells exposed to isotopically labeled ¹⁵N₅-AMP for 3 hours in presence or not of either AB-680 (0.5 μmol/L).

: AB-680 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Nov;10(33):e2302498. [Abstract]; Image, volumes, and weights of the AB-680 (20 mg/kg; i.p.; once weekly for 3 weeks)‐treated versus Control KPC subcutaneous allografts (n = 6 samples per group).

: AB-680 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Nov;10(33):e2302498. [Abstract]; Representative images of H&E staining, Masson's trichrome staining (MTS), and immunohistochemistry staining of CD8, Granzyme B, α‐SMA. Scale bar: 200 µm. The results showed that AB-680 (20 mg/kg; i.p.; once weekly for 3 weeks) significantly increased the numbers of CD8⁺ and Granzyme B⁺ cells.

: AB-680 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Nov;10(33):e2302498. [Abstract]; Representative images of immunohistochemistry staining of CXCL5 and CXCR2 in the KPC tumors from the indicated treatment groups. The results showed that AB-680 (20 mg/kg, i.p.; once weekly) also increased the percentage of the corresponding receptor CXCR2⁺ area in treated tumors.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Ki: 4.9 pM (hCD73)^[1]
IC50: 43 pM (Soluble hCD73)^[1]

In Vitro

AB-680 (AB680) inhibits soluble hCD73 with an IC₅₀ of 0.043 nM, also inhibits hCD73 in CHO cells, suppresses CD73 in human, mouse CD8⁺ T Cells and hPBMC, with IC₅₀s of 0.070, 0.008, 0.66 and 0.011 nM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

580.82

Formula

C₂₀H₂₄ClFN₄O₉P₂

CAS No.

2105904-82-1

Appearance

Solid

Color

White to off-white

SMILES

O=P(CP(O)(O)=O)(O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=C(Cl)C=C3N[C@H](C4=CC=CC=C4F)C)=C3C=N2)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (172.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (8.61 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7217 mL	8.6085 mL	17.2170 mL
5 mM	0.3443 mL	1.7217 mL	3.4434 mL
10 mM	0.1722 mL	0.8609 mL	1.7217 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution
Protocol 5

Add each solvent one by one: 5% DMSO 95% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.30 mM); Suspended solution; Need ultrasonic
Protocol 6

Add each solvent one by one: 1% DMSO 99% Saline
Solubility: ≥ 0.5 mg/mL (0.86 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.71% ee.: 99.96%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (251 KB) HNMR (224 KB) RP-HPLC (111 KB) NP-HPLC (140 KB) LCMS (118 KB)

Handling Instructions (2659 KB)

References

[1]. Lawson KV, et al. Discovery and Characterization of AB680, a Potent and Selective Small-Molecule CD73 Inhibitor for Cancer Immunotherapy.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.7217 mL	8.6085 mL	17.2170 mL	43.0426 mL
H₂O / DMSO	5 mM	0.3443 mL	1.7217 mL	3.4434 mL	8.6085 mL
DMSO	10 mM	0.1722 mL	0.8609 mL	1.7217 mL	4.3043 mL
	15 mM	0.1148 mL	0.5739 mL	1.1478 mL	2.8695 mL
	20 mM	0.0861 mL	0.4304 mL	0.8609 mL	2.1521 mL
	25 mM	0.0689 mL	0.3443 mL	0.6887 mL	1.7217 mL
	30 mM	0.0574 mL	0.2870 mL	0.5739 mL	1.4348 mL
	40 mM	0.0430 mL	0.2152 mL	0.4304 mL	1.0761 mL
	50 mM	0.0344 mL	0.1722 mL	0.3443 mL	0.8609 mL
	60 mM	0.0287 mL	0.1435 mL	0.2870 mL	0.7174 mL
	80 mM	0.0215 mL	0.1076 mL	0.2152 mL	0.5380 mL
	100 mM	0.0172 mL	0.0861 mL	0.1722 mL	0.4304 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

AB-680 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AB-6802105904-82-1AB680AB 680CD73ecto-5′-nucleotidase, NT5EInhibitorinhibitorinhibit

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AB-680

Customer Review

Other Forms of AB-680:

AB-680 Related Antibodies

22 Publications Citing Use of MCE AB-680

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

AB-680 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

AB-680 Related Classifications

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