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EN460 

Cat. No.: HY-12837 Purity: 98.27%
Handling Instructions

EN460 is a selective endoplasmic reticulum oxidation 1 (ERO1) inhibitor. EN460 (IC50 of 1.9 μM) interacts selectively with the reduced, active form of ERO1α and prevents its reoxidation.

For research use only. We do not sell to patients.

EN460 Chemical Structure

EN460 Chemical Structure

CAS No. : 496807-64-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 122 In-stock
Estimated Time of Arrival: December 31
5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 200 In-stock
Estimated Time of Arrival: December 31
25 mg USD 400 In-stock
Estimated Time of Arrival: December 31
50 mg USD 700 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1200 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE EN460

    EN460 purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2019 Jan 31;38(1):44. 

    143B cells are treated with CYT997 (80 nM) and/or EN460 (8 μM) for 24 h, and apoptosis, autophagy and ER stress-related proteins including c-PARP, caspase-4, LC3B, Beclin-1, CHOP and ERO1 are analyzed by western blotting.
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    Description

    EN460 is a selective endoplasmic reticulum oxidation 1 (ERO1) inhibitor. EN460 (IC50 of 1.9 μM) interacts selectively with the reduced, active form of ERO1α and prevents its reoxidation[1].

    In Vitro

    Exposure of cells to EN460 or QM295 resulted in the accumulation of an ERO1α form with lower mobility, indicative of the reduced state. EN460 and QM295 were also found to activate an unfolded protein response reporter in cultured 293T cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    460.79

    Formula

    C₂₂H₁₂ClF₃N₂O₄

    CAS No.

    496807-64-8

    SMILES

    FC(F)(F)C1=NN(C2=CC(C(O)=O)=C(Cl)C=C2)C(/C1=C\C3=CC=C(C4=CC=CC=C4)O3)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 12.5 mg/mL (27.13 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1702 mL 10.8509 mL 21.7019 mL
    5 mM 0.4340 mL 2.1702 mL 4.3404 mL
    10 mM 0.2170 mL 1.0851 mL 2.1702 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 1.25 mg/mL (2.71 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 1.25 mg/mL (2.71 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 1.67 mg/mL (3.62 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    EN460EN 460EN-460OthersInhibitorinhibitorinhibit

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    Product Name:
    EN460
    Cat. No.:
    HY-12837
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