2. Anti-infection
  3. HBV
  4. Entecavir

Entecavir  (Synonyms: BMS200475; SQ34676)

Cat. No.: HY-13623 Purity: 99.98%
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Entecavir (SQ 34676; BMS 200475) is a potent and selective inhibitor of HBV, with an EC50 of 3.75 nM in HepG2 cell.

For research use only. We do not sell to patients.

CAS No. : 142217-69-4

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Customer Review

Based on 19 publication(s) in Google Scholar

Other Forms of Entecavir:

Entecavir Related Antibodies

Top Publications Citing Use of Products

    Entecavir purchased from MedChemExpress. Usage Cited in: Genes Dis. 2025 Sep 23.

    OCR was measured by Seahorse analyzer to determine basal respiration, ATP-linked respiration, proton leak, and maximal respiration capacity in HepG2 and HBV-replication hepatoma cell lines (HepG2.2.15 and HepAD38) with or without Entecavir (ETV: 20 μM) treatment.

    Entecavir purchased from MedChemExpress. Usage Cited in: Genes Dis. 2025 Sep 23.

    MMP was detected using JC-1 dye by flow cytometry in HepG2, HepG2.2.15, and HepAD38 with or without Entecavir (ETV) treatment.

    Entecavir purchased from MedChemExpress. Usage Cited in: Genes Dis. 2025 Sep 23.

    Cellular ROS levels were determined using DCFH-DA dye by flow cytometry in HepG2, HepG2.2.15, and HepAD38 with or without Entecavir (ETV) treatment. Higher MFI of DCFH-DA dye indicated more ROS production in cells.

    Entecavir purchased from MedChemExpress. Usage Cited in: Cell Mol Gastroenterol Hepatol. 2022;13(4):1001-1017.  [Abstract]

    AML12 cells were seeded in 24-well plates at a dose of 5 × 10⁴ vg/cell and transduced with AAV-HBV1.04. Twenty-four hours after transduction, cells were treated with BAY 41-4109 (5 μM), GLS4 (0.2 μM), Entecavir (ETV: 0.5 μM), or dimethyl sulfoxide (DMSO), and cells were collected 7 days after transduction. HBc levels were detected by Western blot.

    Entecavir purchased from MedChemExpress. Usage Cited in: Cell Mol Gastroenterol Hepatol. 2022;13(4):1001-1017.  [Abstract]

    AML12 cells were seeded in 24-well plates at a dose of 5 × 10⁴ vg/cell and transduced with AAV-HBV1.04. Twenty-four hours after transduction, cells were treated with BAY 41-4109 (5 μM), GLS4 (0.2 μM), Entecavir (ETV: 0.5 μM), or dimethyl sulfoxide (DMSO), and cells were collected 7 days after transduction. HBV DNA in the supernatant was detected by qPCR.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Entecavir (SQ 34676; BMS 200475) is a potent and selective inhibitor of HBV, with an EC50 of 3.75 nM in HepG2 cell.

    IC50 & Target

    EC50: 3.75 nM (anti-HBV, HepG2 cell)[2]
    Cellular Effect
    Cell Line Type Value Description References
    HepG2 CC50
    30 μM
    Compound: 76
    Cytotoxicity against human HepG2 cells
    Cytotoxicity against human HepG2 cells
    		[PMID: 36858050]
    HepG2 EC50
    3.75 nM
    Compound: 76
    Antitumor activity against human HepG2 cells assessed as reduction in cell growth
    Antitumor activity against human HepG2 cells assessed as reduction in cell growth
    		[PMID: 36858050]
    HepG2 2.2.15 CC50
    > 1 μM
    Compound: ETV
    Cytotoxicity against human HepG2.2.15 cells harboring HBV genome incubated for 8 days by MTT assay
    Cytotoxicity against human HepG2.2.15 cells harboring HBV genome incubated for 8 days by MTT assay
    		[PMID: 37229833]
    In Vitro

    BMS-200475 has a EC50 of 3.75 nM against HBV. It is incorporated into the protein primer of HBV and subsequently inhibits the priming step of the reverse transcriptase. The antiviral activity of BMS-200475 is significantly less against the other RNA and DNA viruses[1]. Entecavir is more readily phosphorylated to its active metabolites than other deoxyguanosine analogs (penciclovir, ganciclovir, lobucavir, and aciclovir) or lamivudine. The intracellular half-life of entecavir is 15 h[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Entecavir Related Antibodies
    In Vivo

    Daily oral treatment with BMS-200475 at doses ranging from 0.02 to 0.5 mg/kg of body weight for 1 to 3 months effectively reduces the level of woodchuck hepatitis virus (WHV) viremia in chronically infected woodchucks[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    277.28

    Formula

    C12H15N5O3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC(N1)=NC(C2=C1N([C@@H](C[C@H](O)[C@H]3CO)C3=C)C=N2)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 44 mg/mL (158.68 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6065 mL 18.0323 mL 36.0646 mL
    5 mM 0.7213 mL 3.6065 mL 7.2129 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.98%

    SDS (537 KB)

    COA (251 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    BMS 200475 is prepared in phosphate-buffered saline (PBS) and diluted with appropriate medium containing 2% fetal bovine serum. HepG2 2.2.15 cells are plated at a density of 5×105 cells per well on 12-well Biocoat collagen-coated plates and are maintained in a confluent state for 2 to 3 days before being overlaid with 1 mL of medium spiked with BMS 200475. Quantification of HBV was performed on day 10[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6065 mL 18.0323 mL 36.0646 mL 90.1616 mL
    5 mM 0.7213 mL 3.6065 mL 7.2129 mL 18.0323 mL
    10 mM 0.3606 mL 1.8032 mL 3.6065 mL 9.0162 mL
    15 mM 0.2404 mL 1.2022 mL 2.4043 mL 6.0108 mL
    20 mM 0.1803 mL 0.9016 mL 1.8032 mL 4.5081 mL
    25 mM 0.1443 mL 0.7213 mL 1.4426 mL 3.6065 mL
    30 mM 0.1202 mL 0.6011 mL 1.2022 mL 3.0054 mL
    40 mM 0.0902 mL 0.4508 mL 0.9016 mL 2.2540 mL
    50 mM 0.0721 mL 0.3606 mL 0.7213 mL 1.8032 mL
    60 mM 0.0601 mL 0.3005 mL 0.6011 mL 1.5027 mL
    80 mM 0.0451 mL 0.2254 mL 0.4508 mL 1.1270 mL
    100 mM 0.0361 mL 0.1803 mL 0.3606 mL 0.9016 mL
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    Entecavir Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Entecavir142217-69-4BMS200475 SQ34676BMS 200475BMS-200475SQ34676SQ 34676SQ-34676HBVHepatitis B virusInhibitorinhibitorinhibit

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