1. Immunology/Inflammation
  2. Aryl Hydrocarbon Receptor

FICZ (Synonyms: 6-Formylindolo[3,2-b]carbazole)

Cat. No.: HY-12451 Purity: 98.19%
Handling Instructions

FICZ is a potent aryl hydrocarbon receptor (AHR) agonist, and also has the qualities of a perfect substrate for mammalian CYP1 enzymes.

For research use only. We do not sell to patients.

FICZ Chemical Structure

FICZ Chemical Structure

CAS No. : 172922-91-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 338 In-stock
Estimated Time of Arrival: December 31
1 mg USD 108 In-stock
Estimated Time of Arrival: December 31
5 mg USD 420 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

FICZ is a potent aryl hydrocarbon receptor (AHR) agonist, and also has the qualities of a perfect substrate for mammalian CYP1 enzymes.

In Vitro

FICZ (0.01 nM-1 µM) alone or in combination with 50 nM MNF induces sustained CYP1A1 activity and leads to oxidative stress and activation of apoptosis via a mitochondrial-dependent pathway. In HepG2 cells, FICZ stimulates cell growth at low concentrations but inhibits cell growth at high concentrations[1]. FICZ (10,000-30,000 nM) significantly decreases CEH viability with an estimated LC50 (95% confidence intervals) of 14,000 nM. FICZ shows concentration-dependent effects on EROD activity in CEH cultures, with the mean EC50 values at 3, 8, and 24 h of 0.016 nM, 0.80 nM, and 11 nM, respectively[2]. FICZ treatment increases transcript expression of CYP1A1 in a dose-dependent manner in both the parental iPSC line and the CYP1A1 targeted clone[3]. CYP1 inhibition in the presence of FICZ results in enhanced AHR activation, suggesting that FICZ accumulates in the cell when its metabolism is blocked. CYP1 enzymes plays a role in regulating biological effects of FICZ[4].

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (35.17 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5173 mL 17.5864 mL 35.1729 mL
5 mM 0.7035 mL 3.5173 mL 7.0346 mL
10 mM 0.3517 mL 1.7586 mL 3.5173 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[2]

The cell viability of CEH treated with FICZ or TCDD is studied with the untreated cells (used as a live cell control) and sodium hypochlorite (5%)-treated cells (used as a dead cell control). This assay is based upon the bioluminescent measurement of adenosine triphosphate (ATP) that is present in all metabolically active cells. Luciferase is utilized in this method to catalyze the formation of light from ATP and luciferin. CEH are lysed 24 h after dosing and the luminescence emitted from the ATP-dependent oxidation of luciferin is measured with a LuminoSkan Ascent luminometer.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

284.31

Formula

C₁₉H₁₂N₂O

CAS No.

172922-91-7

SMILES

O=CC1=C2C(NC3=C2C=CC=C3)=CC4=C1NC5=C4C=CC=C5

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: 98.19%

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FICZ
Cat. No.:
HY-12451
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FICZ

Cat. No.: HY-12451