1. Immunology/Inflammation
    NF-κB
    Metabolic Enzyme/Protease
  2. Reactive Oxygen Species
  3. HTHQ

HTHQ (Synonyms: 1-O-hexyl-2,3,5-trimethylhydroquinone)

Cat. No.: HY-100768 Purity: 99.89%
Handling Instructions

HTHQ (1-O-hexyl-2,3,5-trimethylhydroquinone) is a potent lipophilic phenolic antioxidant. HTHQ has considerable anti-oxidative activity by directly reacting with reactive oxygen species (ROS) and scavenging ROS to form more stable free radicals.

For research use only. We do not sell to patients.

HTHQ Chemical Structure

HTHQ Chemical Structure

CAS No. : 148081-72-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
50 mg USD 96 In-stock
Estimated Time of Arrival: December 31
100 mg USD 132 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE HTHQ

    HTHQ purchased from MCE. Usage Cited in: J Cell Mol Med. 2020 Jul 17.

    HTHQ against cerebral I/R injury via Nrf2 antioxidant pathway. Western blots showing the level of Nrf2, HO-1, nuclear Nrf2 and cytosolic Nrf2 in mice following cerebral I/R. The expression of Nrf2, HO-1 and nuclear Nrf2 is also significantly increased after HTHQ treatment.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    HTHQ (1-O-hexyl-2,3,5-trimethylhydroquinone) is a potent lipophilic phenolic antioxidant. HTHQ has considerable anti-oxidative activity by directly reacting with reactive oxygen species (ROS) and scavenging ROS to form more stable free radicals[1][2].

    IC50 & Target

    ROS[1]

    In Vitro

    HTHQ (0-100 μM; 24 hours; PC12 cells) treatment increases cell viabilities in a dose-dependent manner[1].
    HTHQ (10 μM; 0.6-24 hours; PC12 cells) inhibits 3,4-L-Dihydroxyphenylalanine (L-DOPA) -induced phosphorylation of sustaines extracellular signal-regulated kinases (ERK1/2), p38 mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase (JNK1/2). HTHQ also normalizes L-DOPA-reduced Bcl-2-associated death protein (Bad) phosphorylation and Bcl-2-associated X protein (Bax) expression, promoting cell survival[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: PC12 cells
    Concentration: 0 μM, 1 μM, 10 μM, and 100 μM
    Incubation Time: 24 hours
    Result: Reduced cell viability at 24 hours caused by both 100 and 200 µM L-DOPA was significantly attenuated.

    Western Blot Analysis[1]

    Cell Line: PC12 cells
    Concentration: 10 μM
    Incubation Time: 0.6-24 hours
    Result: Inhibited L-DOPA-induced phosphorylation of sustained extracellular signal-regulated kinases (ERK1/2), p38 mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase (JNK1/2). And also normalized L-DOPA-reduced Bcl-2-associated death protein (Bad) phosphorylation and Bcl-2-associated X protein (Bax) expression.
    In Vivo

    HTHQ (50-200 mg/kg; oral administration; for 4 weeks; specific pathogen-free male Sprague Dawley rats) treatment significantly improves liver weight and serum chemistry level. HTHQ reduces hydroxyproline and malondialdehyde level in the liver. HTHQ treatment also reduces fibrotic septa. HTHQ administration shows reduced mRNA level of PDGF (Plateletderived growth factor) , α-SMA (α-smooth muscle actin) and TGF-β (transforming growth factor-β) than DMN-induced hepetic fibrosis animals in the liver tissue[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 48 specific pathogen-free male Sprague Dawley (SD) rats (6-week-old ) with dimethylnitrosamine (DMN) [2]
    Dosage: 50 mg/kg, 100 mg/kg, 200 mg/kg
    Administration: Oral administration; for 4 weeks
    Result: Improved against DMN-induced liver fibrosis in male SD rats.
    Molecular Weight

    236.35

    Formula

    C₁₅H₂₄O₂

    CAS No.

    148081-72-5

    SMILES

    OC1=C(C)C=C(OCCCCCC)C(C)=C1C

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (423.10 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.2310 mL 21.1551 mL 42.3101 mL
    5 mM 0.8462 mL 4.2310 mL 8.4620 mL
    10 mM 0.4231 mL 2.1155 mL 4.2310 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 25 mg/mL (105.78 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.89%

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    HTHQ1-O-hexyl-2,3,5-trimethylhydroquinoneReactive Oxygen SpeciesInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email address *

    Phone number *

     

    Organization name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    HTHQ
    Cat. No.:
    HY-100768
    Quantity:
    MCE Japan Authorized Agent: