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Importazole 

Cat. No.: HY-101091 Purity: 99.20%
Handling Instructions

Importazole is a small molecule inhibitor of the nuclear transport receptor importin-β.

For research use only. We do not sell to patients.

Importazole Chemical Structure

Importazole Chemical Structure

CAS No. : 662163-81-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 90 In-stock
Estimated Time of Arrival: December 31
25 mg USD 190 In-stock
Estimated Time of Arrival: December 31
50 mg USD 290 In-stock
Estimated Time of Arrival: December 31
100 mg USD 490 In-stock
Estimated Time of Arrival: December 31
200 mg USD 810 In-stock
Estimated Time of Arrival: December 31
500 mg   Get quote  
1 g   Get quote  

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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Importazole purchased from MCE. Usage Cited in: Nature. 2018 Nov;563(7729):131-136.

    The inhibition of importin-β by Importazole significantly reduces the nuclear translocation of cGAS induced by DNA damage.

    Importazole purchased from MCE. Usage Cited in: Nature. 2018 Nov;563(7729):131-136.

    The inhibition of importin-β by Importazole significantly reduces the nuclear translocation of cGAS induced by DNA damage.

    Importazole purchased from MCE. Usage Cited in: Nature. 2018 Nov;563(7729):131-136.

    The inhibition of importin-β by Importazole significantly reduces the nuclear translocation of cGAS induced by DNA damage.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Importazole is a small molecule inhibitor of the nuclear transport receptor importin-β.

    In Vitro

    Importazole specifically blocks importin-β-mediated nuclear import both in Xenopus egg extracts and cultured cells, without disrupting transportin-mediated nuclear import or CRM1-mediated nuclear export. Importazole impairs the release of an importin-β cargo FRET probe and causes both predicted and novel defects in spindle assembly[1]. Importazole displays an IC50 of approximately 15 μM for inhibition of NFAT-GFP import. Importazole has an IC50 of approximately 22.5 μM in HeLa cells following treatment over a 24-hour period[1]. Importazole induces a dose-and time-dependent inhibition of myeloma cells growth. And the IC50 values of importazole on RPMI 8226 and NCI-H929 after 48 hours incubation are (4.43±0.41) and (4.78±0.35) μmol/L, respectively. Treatment of RPMI 8226 and NCI-H929 cells with 8 μmol/L importazole for 24 h could inhibit NF-κB import to nucleus and reduce its DNA binding activity[2].

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 33 mg/mL (103.64 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1405 mL 15.7025 mL 31.4051 mL
    5 mM 0.6281 mL 3.1405 mL 6.2810 mL
    10 mM 0.3141 mL 1.5703 mL 3.1405 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (7.85 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (7.85 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    HEK 293 cells stably expressing NFAT-GFP are grown on glass coverslips to approximately 50% confluency prior to drug treatment. In all cases, importazole is used at 40 μM and leptomycin B is used at 10 ng/mL. For controls, DMSO is used at a concentration of 0.4%. Ionomycin is added at 1.25 μM. Importazole and leptomycin B treatments are all for 1 hour. In all experiments cells are fixed with 4% formaldehyde prior to fluorescence microscopy. DNA is visualized with 1 μg/mL Hoechst dye. For quantification, 100 cells from each condition are analyzed and the percentage that shows nuclear accumulation of NFAT-GFP are calculated[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    318.42

    Formula

    C₂₀H₂₂N₄

    CAS No.

    662163-81-7

    SMILES

    CC(NC1=C2C=CC=CC2=NC(N3CCCC3)=N1)C4=CC=CC=C4

    Shipping

    Room temperature in continental US; may vary elsewhere

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    Inquiry Information

    Product Name:
    Importazole
    Cat. No.:
    HY-101091
    Quantity:

    Importazole

    Cat. No.: HY-101091