Resmetirom
Based on 22 publication(s) in Google Scholar
Resmetirom (MGL-3196) is a highly selective and orally active thyroid hormone receptor β (THR-β) agonist with an EC50 value of 0.21 μM. Resmetirom can be used for the study of noncirrhotic nonalcoholic steatohepatitis.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 920509-32-6
- Formula: C17H12Cl2N6O4
- Molecular Weight:435.22
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Resmetirom
More- Nat Metab. 2024 Dec;6(12):2281-2299. [Abstract]
- Cell Rep Med. 2025 Apr 15;6(4):102047. [Abstract]
- Acta Biomater. 2025 Aug:202:503-516. [Abstract]
- Phytomedicine. 2026 Jun:155:158009. [Abstract]
- Phytomedicine. 2025 Nov 19:150:157572. [Abstract]
- Br J Pharmacol. 2021 Jun;178(12):2412-2423. [Abstract]
- JHEP Rep. 2026 Feb 20.
- J Med Chem. 2025 Nov 13;68(21):23277-23299. [Abstract]
- Acta Physiol. 2024 Aug 16:e14217. [Abstract]
- Nutrients. 2024 Dec 10;16(24):4260. [Abstract]
- Int J Mol Sci. 2025 Nov 17;26(22):11099. [Abstract]
- Probiotics Antimicrob Proteins. 2026 Jan 17. [Abstract]
- Biochem Biophys Res Commun. 2024 Nov 19:734:150742. [Abstract]
- Biol Pharm Bull. 2025;48(10):1621-1633. [Abstract]
- Biol Pharm Bull. 2025;48(4):463-474. [Abstract]
- Pompeu Fabra University. 2026.
- SSRN. 2025 Nov 8.
- bioRxiv. 2025 Oct 13.
- Explor Endocr Metab Dis. 2025 Jun 24;2:101433.
- Res Sq. 2025 May 25.
- bioRxiv. 2024 May 7.
- bioRxiv. 2024 Feb 2.
Customer Validation & Images
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ELISA
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RT-PCR
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Bio/Physico-chemical Assay
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Histological Imaging/Staining
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ELISA
Biological Activity
EC50: 0.21 μM (THR-β)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | EC50 |
303 nM
Compound: 4
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Effect on CPT1A gene expression in human Huh-7 cells measured for 24 hrs
Effect on CPT1A gene expression in human Huh-7 cells measured for 24 hrs
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[PMID: 39221768] |
Resmetirom (MGL-3196) is 28-fold selective for THR-β (EC50=0.21 μM) over THR-α (EC50=3.74 μM ) in a functional assay. Resmetirom (MGL-3196) shows an IC20 of roughly 30 μM for blockage of the hERG channel. The IC50 for CYP3A4/5 and for CYP2C19 is >50 μM, and there is only weak inhibition (roughly 22 μM) of CYP2C9[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 920509-32-6
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Appearance Solid
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Molecular Weight 435.22
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Formula C17H12Cl2N6O4
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Color White to yellow
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SMILES
N#CC1=NN(C2=CC(Cl)=C(OC(C=C3C(C)C)=NNC3=O)C(Cl)=C2)C(NC1=O)=O
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Synonyms
MGL-3196; VIA-3196
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (22)
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Journal Impact Factor
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Most Recent
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Nat Metab
2024 Dec;6(12):2281-2299. PMID: 39578649
Resmetirom purchased from MedChemExpress. Usage Cited in: Nat Metab. 2024 Dec;6(12):2281-2299. [Abstract]
The experimental schedule showing the treatment of female 8-week-old C57BL/6J mice with Resmetirom (RES) (0.033 mg /mL or vehicle control per gavage). RES was prepared in 10% DMSO, 40% PEG300, 5% Tween 80 and 45% drinking water (v/v), at a final concentration of 0.033 mg /mL.
Resmetirom purchased from MedChemExpress. Usage Cited in: Nat Metab. 2024 Dec;6(12):2281-2299. [Abstract]
The experimental schedule showing the treatment of female 8-week-old C57BL/6J mice with Resmetirom (RES) (0.033 mg /mL or vehicle control per gavage). RES was prepared in 10% DMSO, 40% PEG300, 5% Tween 80 and 45% drinking water (v/v), at a final concentration of 0.033 mg /mL.
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Cell Rep Med
Treatment of IL-18-binding protein biologics suppresses fibrotic progression in metabolic dysfunction-associated steatohepatitis. [Abstract]2025 Apr 15;6(4):102047. PMID: 40239621
Resmetirom purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Apr 15;6(4):102047. [Abstract]
C57BL/6J wild-type mice were fed FPC diet for 27 weeks. Drugs were administered from 19 to 27 weeks. 8 mg/kg of APB-R3 (SAFA-hIL-18BP) was intraperitoneally administered three times a week as indicated. 5 mg/kg of THRβ agonist (MGL-3196; Resmetirom) was daily orally gavaged as compared group. Plasma ALT and AST levels were measured at the end of experiments.
Resmetirom purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Apr 15;6(4):102047. [Abstract]
C57BL/6J wild-type mice were fed FPC diet for 27 weeks. Drugs were administered from 19 to 27 weeks. 8 mg/kg of APB-R3 (SAFA-hIL-18BP) was intraperitoneally administered three times a week as indicated. 5 mg/kg of THRβ agonist (MGL-3196; Resmetirom) was daily orally gavaged as compared group. Representative images of H&E liver sections.
Resmetirom purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Apr 15;6(4):102047. [Abstract]
C57BL/6J wild-type mice were fed FPC diet for 27 weeks. Drugs were administered from 19 to 27 weeks. 8 mg/kg of APB-R3 (SAFA-hIL-18BP) was intraperitoneally administered three times a week as indicated. 5 mg/kg of THRβ agonist (MGL-3196; Resmetirom) was daily orally gavaged as compared group. ELISA assay was conducted to measure plasma Pro-C3.
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Acta Biomater
A ROS/ultrasound dual-responsive nanocarrier enhances drug penetration for ameliorating metabolic dysfunction-associated steatohepatitis. [Abstract]2025 Aug:202:503-516. PMID: 40617493 -
Phytomedicine
Myricetin stabilizes PINK1 to activate mitophagy and ameliorate metabolic dysfunction-associated steatotic liver disease. [Abstract]2026 Jun:155:158009. PMID: 41935459 -
Phytomedicine
Quzhou-sourced Fructus Aurantii ameliorates MASLD by modulating glycolysis-dependent M1 polarization in hepatic macrophages. [Abstract]2025 Nov 19:150:157572. PMID: 41442991 -
Br J Pharmacol
Activation of thyroid hormone receptor-β improved disease activity and metabolism independent of body weight in a mouse model of non-alcoholic steatohepatitis and fibrosis. [Abstract]2021 Jun;178(12):2412-2423. PMID: 33655500 -
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J Med Chem
Discovery of Novel P2Y14R Inhibitors for Ameliorating Liver Fibrosis by Suppressing Hepatic Stellate Cell Activation. [Abstract]2025 Nov 13;68(21):23277-23299. PMID: 41178760 -
Acta Physiol
Beneficial effects of MGL-3196 and BAM15 combination in a mouse model of fatty liver disease. [Abstract]2024 Aug 16:e14217. PMID: 39152636 -
Nutrients
Lactobacillus delbrueckii subsp. lactis CKDB001 Ameliorates Metabolic Complications in High-Fat Diet-Induced Obese Mice. [Abstract]2024 Dec 10;16(24):4260. PMID: 39770882 -
Int J Mol Sci
2025 Nov 17;26(22):11099. PMID: 41303582 -
Probiotics Antimicrob Proteins
Lactobacillus Plantarum Q180 Attenuates Hepatic Lipid Accumulation and Regulates Energy Metabolism in High-Fat Diet-Induced Obese Mice. [Abstract]2026 Jan 17. PMID: 41546789 -
Biochem Biophys Res Commun
Liver-specific thyroid hormone receptor-β agonism alleviates alcoholic steatohepatitis (ASH) in mice. [Abstract]2024 Nov 19:734:150742. PMID: 39353359 -
Biol Pharm Bull
Effects of Resmetirom on Resistance to Thyroid Hormone Receptor Mutants: Potential Basis for Therapeutic Applications. [Abstract]2025;48(10):1621-1633. PMID: 41161771 -
Biol Pharm Bull
Resmetirom Is an Effective Thyromimetics for the Chemical Rescue of Thyroid Hormone Receptor Mutants. [Abstract]2025;48(4):463-474. PMID: 40301009 -
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Solvent & Solubility
DMSO : 70 mg/mL (160.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.75 mg/mL (8.62 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.75 mg/mL (8.62 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Rats[1]
Resmetirom (MGL-3196), compounds 54 and 55 are formulated in 4% DMSO, 15% PEG-400, and 81% of 30% HPBCD in phosphate buffer and are administered intraperitoneally. For MGL-3196 and 54, 4 rats per group are tested at 5, 20, and 37.5 mg/kg. For 55, 3 rats per group are tested at 5 and 15 mg/kg and 4 rats are tested at 50 mg/kg[1].
Mice[1]
Six week old C57Bl/6J mice are placed on a high fat diet for 34 weeks. At day 0, 9 mice per group are treated daily doses by gavage with vehicle (2% Klucel LF, 0.1% Tween 80 in water) or 0.3, 1, 3, or 10 mg/kg Resmetirom (MGL-3196) for 23 days. In a parallel study, at day 0, 9 mice per group are treated with daily doses of vehicle (Dulbecco’s phosphate buffered saline, pH adjusted to 9.0 with 1 N NaOH) or 10, 30, or 100 μg/kg T3[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Kelly MJ, et al. Discovery of 2-[3,5-dichloro-4-(5-isopropyl-6-oxo-1,6-dihydropyridazin-3-yloxy)phenyl]-3,5-dioxo-2,3,4,5-tetrahydro[1,2,4]triazine-6-carbonitrile (MGL-3196), a Highly Selective Thyroid Hormone Receptor β agonist in clinical trials for the treatment of dyslipidemia. J Med Chem. 2014 May 22;57(10):3912-23. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2977 mL | 11.4884 mL | 22.9769 mL | 57.4422 mL |
| 5 mM | 0.4595 mL | 2.2977 mL | 4.5954 mL | 11.4884 mL | |
| 10 mM | 0.2298 mL | 1.1488 mL | 2.2977 mL | 5.7442 mL | |
| 15 mM | 0.1532 mL | 0.7659 mL | 1.5318 mL | 3.8295 mL | |
| 20 mM | 0.1149 mL | 0.5744 mL | 1.1488 mL | 2.8721 mL | |
| 25 mM | 0.0919 mL | 0.4595 mL | 0.9191 mL | 2.2977 mL | |
| 30 mM | 0.0766 mL | 0.3829 mL | 0.7659 mL | 1.9147 mL | |
| 40 mM | 0.0574 mL | 0.2872 mL | 0.5744 mL | 1.4361 mL | |
| 50 mM | 0.0460 mL | 0.2298 mL | 0.4595 mL | 1.1488 mL | |
| 60 mM | 0.0383 mL | 0.1915 mL | 0.3829 mL | 0.9574 mL | |
| 80 mM | 0.0287 mL | 0.1436 mL | 0.2872 mL | 0.7180 mL | |
| 100 mM | 0.0230 mL | 0.1149 mL | 0.2298 mL | 0.5744 mL |
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