NIBR189

Name: NIBR189
Brand: MedChemExpress
SKU: HY-12336
Rating: 5.0 (3 reviews)

Cat. No.: HY-12336 Purity: 99.71%: Data Sheet
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NIBR189 is an EBI2 (Epstein-Barr virus-induced gene 2) antagonist. NIBR189 inhibits human and mouse EBI2 with IC₅₀s of 11 and 16 nM, respectively. NIBR189 can be used for the research of autoimmune diseases.

For research use only. We do not sell to patients.

CAS No. : 1599432-08-2

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE NIBR189

Cell Proliferation/Viability Assay

Flow Cytometry

Microbiological Assay

: NIBR189 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2025 May;5(5):831-847. [Abstract]; Relative mito-Dendra2 expression following NIBR189 (25 μg/mL; 48 h) and/or 7α,25-DHC treatment, with normalized MFI values (n = 3).

: NIBR189 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2020 Aug;145:104234. [Abstract]; The cell viability under the treatment of NIBR189 (6.4-25 μM; 24-72 h) and 7α,25-OHC at different time points changes little, determined by MTS assay (n = 5).

: NIBR189 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2020 Aug;145:104234. [Abstract]; Bacterial early infection (4 h poi) is compromised under the treatment of NIBR189 (25 nM) and 7α,25-OHC in RAW264.7. (CFU in left panel, TTD in right panel).

: NIBR189 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2020 Aug;145:104234. [Abstract]; The effect of NIBR189 (25 nM) and 7α,25-OHC on intracellular Mtb replication is compared using cfu at different time points after infection in RAW264.7 (n = 5). NIBR189 reduced the intracellular Mtb at the stages after phagocytosis.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	11 nM Compound: 4m, NIBR189	Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of NIBR51-induced intracellular calcium release measured for 3 mins by FLIPR assay Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of NIBR51-induced intracellular calcium release measured for 3 mins by FLIPR assay	[PMID: 24678947]
CHO	IC₅₀	15 nM Compound: 4m, NIBR189	Antagonist activity at recombinant mouse EBI2 receptor expressed in CHO cells assessed as inhibition of NIBR51-induced intracellular calcium release measured for 3 mins by FLIPR assay Antagonist activity at recombinant mouse EBI2 receptor expressed in CHO cells assessed as inhibition of NIBR51-induced intracellular calcium release measured for 3 mins by FLIPR assay	[PMID: 24678947]
CHO	IC₅₀	7 nM Compound: 4m, NIBR189	Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of 0.1 nM oxysterol-induced [35S]GTPgammaS binding after 1 hr by scintillation counting analysis Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of 0.1 nM oxysterol-induced [35S]GTPgammaS binding after 1 hr by scintillation counting analysis	[PMID: 24678947]
CHO	IC₅₀	8.5 nM Compound: 4m, NIBR189	Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of 0.33 nM oxysterol-induced [35S]GTPgammaS binding after 1 hr by scintillation counting analysis Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of 0.33 nM oxysterol-induced [35S]GTPgammaS binding after 1 hr by scintillation counting analysis	[PMID: 24678947]
U-937	IC₅₀	0.3 nM Compound: 4m, NIBR189	Antagonist activity at EBI2 receptor in human U937 cells assessed as inhibition of 7-alpha,25-OHC-induced cell migration after 3 hrs by flow cytometric analysis Antagonist activity at EBI2 receptor in human U937 cells assessed as inhibition of 7-alpha,25-OHC-induced cell migration after 3 hrs by flow cytometric analysis	[PMID: 24678947]
U-937	IC₅₀	4.5 nM Compound: 4; NIBR189	Antimigratory activity against human U-937 cells assessed as inhibition of 7alpha,25-dihydroxycholesterol induced cell migration by CCK-8 method Antimigratory activity against human U-937 cells assessed as inhibition of 7alpha,25-dihydroxycholesterol induced cell migration by CCK-8 method	[PMID: 38417036]
U-937	IC₅₀	9 nM Compound: 4m, NIBR189	Antagonist activity at EBI2 receptor in human U937 cells assessed as inhibition of oxysterol-induced calcium release measured for 3 mins by FLIPR assay Antagonist activity at EBI2 receptor in human U937 cells assessed as inhibition of oxysterol-induced calcium release measured for 3 mins by FLIPR assay	[PMID: 24678947]

In Vitro

NIBR189 (0-1 μM; 3 h) blocks migration of U937 cells^[1].
NIBR189 (0-10 μM) blocks oxysterol-dependent activation with an IC₅₀ value of 9 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Migration Assay ^[1]

Cell Line:	U937 cell lines
Concentration:	0-1 μM
Incubation Time:	3 hours
Result:	Blocked the direct migration of U937 cells with an IC₅₀ value of 0.3 nM.

In Vivo

1.19 Pharmacokinetic Properties of NIBR189 in Mice^[1].

	Mice IV 1 mg/kg	Mice PO 3 mg/kg
CL (μL/min/mg)	16
t_1/2 (h)	1.1
V_ss (L/kg)	1.4
AUC (nmol·h/L)	2435	3608
C_max (nmol/L)		835
t_max (h)		1
F (%)		49

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

429.31

Formula

C₂₁H₂₁BrN₂O₃

CAS No.

1599432-08-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCN(C(C2=CC=C(OC)C=C2)=O)CC1)/C=C/C3=CC=C(Br)C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (116.47 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3293 mL	11.6466 mL	23.2932 mL
5 mM	0.4659 mL	2.3293 mL	4.6586 mL
10 mM	0.2329 mL	1.1647 mL	2.3293 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.82 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.71%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (244 KB) RP-HPLC (249 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Gessier F, et al. Identification and characterization of small molecule modulators of the Epstein-Barr virus-induced gene 2 (EBI2) receptor. J Med Chem. 2014 Apr 24;57(8):3358-68. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3293 mL	11.6466 mL	23.2932 mL	58.2330 mL
	5 mM	0.4659 mL	2.3293 mL	4.6586 mL	11.6466 mL
	10 mM	0.2329 mL	1.1647 mL	2.3293 mL	5.8233 mL
	15 mM	0.1553 mL	0.7764 mL	1.5529 mL	3.8822 mL
	20 mM	0.1165 mL	0.5823 mL	1.1647 mL	2.9116 mL
	25 mM	0.0932 mL	0.4659 mL	0.9317 mL	2.3293 mL
	30 mM	0.0776 mL	0.3882 mL	0.7764 mL	1.9411 mL
	40 mM	0.0582 mL	0.2912 mL	0.5823 mL	1.4558 mL
	50 mM	0.0466 mL	0.2329 mL	0.4659 mL	1.1647 mL
	60 mM	0.0388 mL	0.1941 mL	0.3882 mL	0.9705 mL
	80 mM	0.0291 mL	0.1456 mL	0.2912 mL	0.7279 mL
	100 mM	0.0233 mL	0.1165 mL	0.2329 mL	0.5823 mL

NIBR189 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NIBR1891599432-08-2NIBR 189NIBR-189EBVEBI2/GPR183Epstein-Barr Virussmall moleculeG proteinautoimmune diseasesmigrationU937autoimmunemiceInhibitorinhibitorinhibit

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NIBR189

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NIBR189 Related Antibodies

3 Publications Citing Use of MCE NIBR189

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Complete Stock Solution Preparation Table

NIBR189 Related Classifications

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