1. Cell Cycle/DNA Damage
  2. RAD51
  3. RI-1

RI-1 

Cat. No.: HY-15317 Purity: 99.83%
Handling Instructions

RI-1 is a RAD51 inhibitor, with IC50s ranging from 5 to 30 μM. RI-1 binds covalently to the surface of RAD51 protein at cysteine 319. RI-1 inactivates RAD51 by directly binding to a protein surface that serves as an interface between protein subunits in RAD51 filaments. RI-1 can disrupt homologous recombination in human cells.

For research use only. We do not sell to patients.

RI-1 Chemical Structure

RI-1 Chemical Structure

CAS No. : 415713-60-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 83 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 83 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 75 In-stock
Estimated Time of Arrival: December 31
10 mg USD 130 In-stock
Estimated Time of Arrival: December 31
50 mg USD 250 In-stock
Estimated Time of Arrival: December 31
100 mg USD 450 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

RI-1 is a RAD51 inhibitor, with IC50s ranging from 5 to 30 μM. RI-1 binds covalently to the surface of RAD51 protein at cysteine 319. RI-1 inactivates RAD51 by directly binding to a protein surface that serves as an interface between protein subunits in RAD51 filaments. RI-1 can disrupt homologous recombination in human cells[1].

IC50 & Target

IC50: 5-30 μM (RAD51)[1]

In Vitro

RI-1 (1-50 µM; 24 h) specifically inhibits homologous recombination (HR) in U2OS cells and stimulates single-strand annealing (SSA) in HEK293 cells[1].
RI-1 (5-20 µM; 30 min) inhibits HsRAD51 in a concentration-dependent manner[1].
RI-1 (20 µM; 8 h) disrupts the formation of RAD51 foci after DNA damage in immortalized human fibroblasts[1].
RI-1 (15-25 µM; 24 h) sensitizes human cancer cells (HeLa, MCF-7 and U2OS) to cross-linking chemotherapy[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RI-1 (50 mg/kg; i.p. every 3 d for 30 d) significantly reduces triple negative breast cancer (TNBC) tumor growth in mice[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c nude mice (6 weeks) bearing TNBC tumor[2]
Dosage: 50 mg/kg
Administration: I.p. every 3 days for 30 days
Result: Resulted in significant inhibition of tumor growth.
Did not cause body weight loss significantly.
Molecular Weight

361.61

Formula

C₁₄H₁₁Cl₃N₂O₃

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (92.17 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7654 mL 13.8271 mL 27.6541 mL
5 mM 0.5531 mL 2.7654 mL 5.5308 mL
10 mM 0.2765 mL 1.3827 mL 2.7654 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.91 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.83%

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RI-1
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HY-15317
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