2. Cell Cycle/DNA Damage
  3. RAD51
  4. RS-1

RS-1 is a RAD51 activator. RS-1 enhances the homologous recombination activity of human RAD51 by promoting the formation of active presynaptic filaments.

For research use only. We do not sell to patients.

CAS No. : 312756-74-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

RS-1 Related Antibodies

Top Publications Citing Use of Products

    RS-1 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 3;26(9):4361.  [Abstract]

    Cell viability of HeLa-eGFPd2 cells treated with 1738-based Cas9 RNP/ssDNA complexes (18.75 nM, RNP) with various gene correction enhancers, including Pevonedistat (0.5, 1, 2 nM), Rs-1 (1, 2.5, 10 nM), SCR7 (1, 5, 10 nM), M3814 (5, 10, 20 nM), KU-0060648 (0.1, 0.25, 1 nM), and Nu7441 (2.5, 5, 7.5 nM). 1738 refers to treatment with 1738 Cas9 RNP/ssDNA complexes without enhancer. Enhancer abbreviations represent the enhancer name followed by its concentration in nM.

    RS-1 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 3;26(9):4361.  [Abstract]

    Total gene editing efficiency (both HDR and NHEJ events) of HeLa-eGFPd2 cells treated with 1738-based Cas9 RNP/ssDNA complexes (18.75 nM, RNP) with various gene correction enhancers, including Pevonedistat (0.5, 1, 2 nM), Rs-1 (1, 2.5, 10 nM), SCR7 (1, 5, 10 nM), M3814 (5, 10, 20 nM), KU-0060648 (0.1, 0.25, 1 nM), and Nu7441 (2.5, 5, 7.5 nM). 1738 refers to treatment with 1738 Cas9 RNP/ssDNA complexes without enhancer. Enhancer abbreviations represent the enhancer name followed by its concentration in nM.

    RS-1 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 May 3;26(9):4361.  [Abstract]

    HDR efficiency of HeLa-eGFPd2 cells treated with 1738-based Cas9 RNP/ssDNA complexes (18.75 nM, RNP) with various gene correction enhancers, including Pevonedistat (0.5, 1, 2 nM), Rs-1 (1, 2.5, 10 nM), SCR7 (1, 5, 10 nM), M3814 (5, 10, 20 nM), KU-0060648 (0.1, 0.25, 1 nM), and Nu7441 (2.5, 5, 7.5 nM). 1738 refers to treatment with 1738 Cas9 RNP/ssDNA complexes without enhancer. Enhancer abbreviations represent the enhancer name followed by its concentration in nM.

    RS-1 purchased from MedChemExpress. Usage Cited in: DNA Repair. 2024 Nov 2:144:103781.  [Abstract]

    HE staining showing the crypt-villus structure in jejunum in mice treated with different doses of RS-1 (50-150 mg/kg; single dose). Scale bars, 100μm. The results showed that a single administration of the highest dose of RS-1 did not result in significant crypt cell death, nor did it affect cell proliferation.

    RS-1 purchased from MedChemExpress. Usage Cited in: DNA Repair. 2024 Nov 2:144:103781.  [Abstract]

    The representative images of IF show Olfm4+ cells in the small intestine of NO IR, IR+Veh and IR+RS-1 (100 mg/kg) groups at 48 h or 84 h after TBI. Scale bars (yellow), 200μm. Scale bars (white), 50μm.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    RS-1 is a RAD51 activator. RS-1 enhances the homologous recombination activity of human RAD51 by promoting the formation of active presynaptic filaments[1].

    IC50 & Target

    RAD51[1][2]
    In Vitro

    RS-1 is a RAD51 activator, stimulating binding of hRAD51 to DNA with Kd ranging from 48 nM to 107 nM in the presence of ATP or ADP and in the absence of a nucleotide cofactor, and such an effect is not via inhibiting its ATPase activity. RS-1 (20 μM) affects the length and helical pitch of hRAD51 protein-DNA complexes. RS-1 (0, 1, 5, 10, 15, 20, and 25 μM) stimulates strand assimilation activity of hRAD51. RS-1 (7.5 μM) promotes resistance of human cells to cross-linking chemotherapy[1]. RS-1 (0, 7.5, 15 μM) increases Cas9-mediated knock-in efficiencies in rabbit embryos[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    RS-1 Related Antibodies
    Clinical Trial
    Molecular Weight

    524.23

    Formula

    C20H16Br2N2O3S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NC1=CC=C(Br)C=C1)C2=CC=C(Br)C(S(=O)(NCC3=CC=CC=C3)=O)=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (190.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9076 mL 9.5378 mL 19.0756 mL
    5 mM 0.3815 mL 1.9076 mL 3.8151 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 3 mg/mL (5.72 mM); Clear solution

      This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 3 mg/mL (5.72 mM); Clear solution

      This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.77%

    SDS (393 KB)

    COA (247 KB) HNMR (256 KB) LCMS (268 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    Briefly, 15 μL reaction volumes include a DNA strand exchange protein (0.8 μM) that is preincubated for 5 min at 37°C with 1 μM (nucleotide concentration) 32P-labeled oligonucleotide 306.7 in a reaction buffer containing 20 mM Hepes (pH 7.5), 1 mM DTT, 2 mM nucleotide cofactor, and 1 mM MgCl2 and various concentrations of RS-1. For experimental buffer conditions that included calcium, 1 mM CaCl2 is present in addition to (in the case of hRAD51) or in the place of (in the case of RecA and scRAD51) the 1 mM MgCl2. Conditions with scRAD51 additionally contains 110 nM scRAD54. After this initial binding reaction, 10 μL of 19.75 μM (base pair concentration) supercoiled homologuecontaining target plasmid DNA (pRS306) is next added along with sufficient magnesium acetate to give a final concentration of 10 mM[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9076 mL 9.5378 mL 19.0756 mL 47.6890 mL
    5 mM 0.3815 mL 1.9076 mL 3.8151 mL 9.5378 mL
    10 mM 0.1908 mL 0.9538 mL 1.9076 mL 4.7689 mL
    15 mM 0.1272 mL 0.6359 mL 1.2717 mL 3.1793 mL
    20 mM 0.0954 mL 0.4769 mL 0.9538 mL 2.3844 mL
    25 mM 0.0763 mL 0.3815 mL 0.7630 mL 1.9076 mL
    30 mM 0.0636 mL 0.3179 mL 0.6359 mL 1.5896 mL
    40 mM 0.0477 mL 0.2384 mL 0.4769 mL 1.1922 mL
    50 mM 0.0382 mL 0.1908 mL 0.3815 mL 0.9538 mL
    60 mM 0.0318 mL 0.1590 mL 0.3179 mL 0.7948 mL
    80 mM 0.0238 mL 0.1192 mL 0.2384 mL 0.5961 mL
    100 mM 0.0191 mL 0.0954 mL 0.1908 mL 0.4769 mL
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    RS-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    RS-1312756-74-4RS1RS 1RAD51Inhibitorinhibitorinhibit

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    Cat. No.:
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