1. Cell Cycle/DNA Damage
  2. RAD51
    CRISPR/Cas9

RS-1 

Cat. No.: HY-19793 Purity: 99.42%
Handling Instructions

RS-1 is a RAD51 activator, and also increases CRISPR/Cas9-mediated knock-in efficiencies.

For research use only. We do not sell to patients.
RS-1 Chemical Structure

RS-1 Chemical Structure

CAS No. : 312756-74-4

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 83 In-stock
5 mg USD 72 In-stock
10 mg USD 102 In-stock
50 mg USD 306 In-stock
100 mg USD 552 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Related Screening Libraries:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

RS-1 is a RAD51 activator, and also increases CRISPR/Cas9-mediated knock-in efficiencies.

IC50 & Target

RAD51[1], CRISPR/Cas9[2]

In Vitro

RS-1 is a RAD51 activator, stimulating binding of hRAD51 to DNA with Kd ranging from 48 nM to 107 nM in the presence of ATP or ADP and in the absence of a nucleotide cofactor, and such an effect is not via inhibiting its ATPase activity. RS-1 (20 μM) affects the length and helical pitch of hRAD51 protein-DNA complexes. RS-1 (0, 1, 5, 10, 15, 20, and 25 μM) stimulates strand assimilation activity of hRAD51. RS-1 (7.5 μM) promotes resistance of human cells to cross-linking chemotherapy[1]. RS-1 (0, 7.5, 15 μM) increases Cas9-mediated knock-in efficiencies in rabbit embryos[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.9076 mL 9.5378 mL 19.0756 mL
5 mM 0.3815 mL 1.9076 mL 3.8151 mL
10 mM 0.1908 mL 0.9538 mL 1.9076 mL
Please refer to the solubility information to select the appropriate solvent.
Kinase Assay
[1]

Briefly, 15 μL reaction volumes include a DNA strand exchange protein (0.8 μM) that is preincubated for 5 min at 37°C with 1 μM (nucleotide concentration) 32P-labeled oligonucleotide 306.7 in a reaction buffer containing 20 mM Hepes (pH 7.5), 1 mM DTT, 2 mM nucleotide cofactor, and 1 mM MgCl2 and various concentrations of RS-1. For experimental buffer conditions that included calcium, 1 mM CaCl2 is present in addition to (in the case of hRAD51) or in the place of (in the case of RecA and scRAD51) the 1 mM MgCl2. Conditions with scRAD51 additionally contains 110 nM scRAD54. After this initial binding reaction, 10 μL of 19.75 μM (base pair concentration) supercoiled homologuecontaining target plasmid DNA (pRS306) is next added along with sufficient magnesium acetate to give a final concentration of 10 mM[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

524.23

Formula

C₂₀H₁₆Br₂N₂O₃S

CAS No.

312756-74-4

SMILES

O=C(NC1=CC=C(Br)C=C1)C2=CC=C(Br)C(S(=O)(NCC3=CC=CC=C3)=O)=C2

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
RS-1
Cat. No.:
HY-19793
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