2. Cell Cycle/DNA Damage
  3. RAD51 CRISPR/Cas9
  4. RS-1

RS-1 

Cat. No.: HY-19793 Purity: 98.95%
COA Handling Instructions

RS-1 is a RAD51 activator, and also increases CRISPR/Cas9-mediated knock-in efficiencies.

For research use only. We do not sell to patients.

RS-1 Chemical Structure

RS-1 Chemical Structure

CAS No. : 312756-74-4

Size Price Stock Quantity
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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 69 In-stock
Solution
10 mM * 1 mL in DMSO USD 69 In-stock
Solid
5 mg USD 60 In-stock
10 mg USD 90 In-stock
50 mg USD 270 In-stock
100 mg USD 450 In-stock
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500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

RS-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE RS-1

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

RS-1 is a RAD51 activator, and also increases CRISPR/Cas9-mediated knock-in efficiencies.

IC50 & Target

RAD51[1], CRISPR/Cas9[2]
In Vitro

RS-1 is a RAD51 activator, stimulating binding of hRAD51 to DNA with Kd ranging from 48 nM to 107 nM in the presence of ATP or ADP and in the absence of a nucleotide cofactor, and such an effect is not via inhibiting its ATPase activity. RS-1 (20 μM) affects the length and helical pitch of hRAD51 protein-DNA complexes. RS-1 (0, 1, 5, 10, 15, 20, and 25 μM) stimulates strand assimilation activity of hRAD51. RS-1 (7.5 μM) promotes resistance of human cells to cross-linking chemotherapy[1]. RS-1 (0, 7.5, 15 μM) increases Cas9-mediated knock-in efficiencies in rabbit embryos[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RS-1 Related Antibodies
Molecular Weight

524.23

Appearance

Solid

Formula

C20H16Br2N2O3S
CAS No.
SMILES

O=C(NC1=CC=C(Br)C=C1)C2=CC=C(Br)C(S(=O)(NCC3=CC=CC=C3)=O)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (190.76 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9076 mL 9.5378 mL 19.0756 mL
5 mM 0.3815 mL 1.9076 mL 3.8151 mL
10 mM 0.1908 mL 0.9538 mL 1.9076 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3 mg/mL (5.72 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 3 mg/mL (5.72 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 98.95%

COA (247 KB) LCMS (269 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

Briefly, 15 μL reaction volumes include a DNA strand exchange protein (0.8 μM) that is preincubated for 5 min at 37°C with 1 μM (nucleotide concentration) 32P-labeled oligonucleotide 306.7 in a reaction buffer containing 20 mM Hepes (pH 7.5), 1 mM DTT, 2 mM nucleotide cofactor, and 1 mM MgCl2 and various concentrations of RS-1. For experimental buffer conditions that included calcium, 1 mM CaCl2 is present in addition to (in the case of hRAD51) or in the place of (in the case of RecA and scRAD51) the 1 mM MgCl2. Conditions with scRAD51 additionally contains 110 nM scRAD54. After this initial binding reaction, 10 μL of 19.75 μM (base pair concentration) supercoiled homologuecontaining target plasmid DNA (pRS306) is next added along with sufficient magnesium acetate to give a final concentration of 10 mM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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RS-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

RS-1312756-74-4RS1RS 1RAD51CRISPR/Cas9Inhibitorinhibitorinhibit

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