2. Cell Cycle/DNA Damage Anti-infection
  3. SRPK Virus Protease
  4. SRPIN340

SRPIN340  (Synonyms: SRPK inhibitor)

Cat. No.: HY-13949 Purity: 99.97%
COA Handling Instructions

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SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1.

For research use only. We do not sell to patients.

SRPIN340 Chemical Structure

SRPIN340 Chemical Structure

CAS No. : 218156-96-8

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 87 In-stock
Solution
10 mM * 1 mL in DMSO USD 87 In-stock
Solid
5 mg USD 79 In-stock
10 mg USD 132 In-stock
50 mg USD 449 In-stock
100 mg USD 581 In-stock
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Based on 8 publication(s) in Google Scholar

SRPIN340 Related Antibodies

Top Publications Citing Use of Products

    SRPIN340 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2022 Oct 25;15(757):eabm0808.  [Abstract]

    Representative primary human type II pneumocyte culture images from samples that are pretreated with SRPIN340 (53 μM; 12 h) before they are infected with SARS-CoV-2. 24 hours later, the cells are fixed and stained for SARS-CoV-2, DNA with DAPI, and surfactant protein C (SFTPC). Scale bar, 100 μm.

    SRPIN340 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2019 Feb 26;510(1):97-103.   [Abstract]

    Bcl-2 protein expression and cleaved caspase-3 protein expression are analyzed by Western blot with or without the treatment of SRPIN340.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1.

    IC50 & Target

    Ki: 0.89 μM (SRPK1)[1]
    In Vitro

    SRPIN340 is a serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1. SRPIN340 also inhibits SRPK2, but shows no significant inhibition on other SRPK, such as Clk1 and Clk4. SRPIN340 promotes degradation of SRp75, which is necessary for HIV expression. SRPIN340 suppresses the propagation of Sindbis virus (IC50, 60 μM) as well as severe acute respiratory syndrome virus[1]. SRPIN340 shows inhibitory effect on leukemia cell lines, such as AML HL60, ALL-T Molt4 and Jurkat, with IC50s of 44.7 μM, 92.2 μM and 82.3 μM, respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SRPIN340 Related Antibodies
    Molecular Weight

    349.35

    Formula

    C18H18F3N3O
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(NC1=CC(C(F)(F)F)=CC=C1N2CCCCC2)C3=CC=NC=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 42 mg/mL (120.22 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8625 mL 14.3123 mL 28.6246 mL
    5 mM 0.5725 mL 2.8625 mL 5.7249 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.16 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.97%

    COA (252 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    Leukemic cells (5 × 104 cells/well) and isolated PBMCs (8 × 104 cells/well) are seeded in 96-well plates. Each well contained 100 μL of complete RPMI medium and 100 μL of SRPIN340 solution at different concentrations. The compound is diluted in RPMI medium with 10% fetal bovine serum and 0.4% DMSO (v/v). After 48 h of culture, MTT (5 mg/mL) is added to the wells (3 h, 37°C). The plates are centrifuged at room temperature for 30 min 500 ×g, followed by the removal of the MTT solution and the addition of 100 μL/well of DMSO to solubilize the formazan. Absorbance is measured at 540 nm in a microplate reader. Each experimental procedure is performed in triplicate[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.8625 mL 14.3123 mL 28.6246 mL 71.5615 mL
    5 mM 0.5725 mL 2.8625 mL 5.7249 mL 14.3123 mL
    10 mM 0.2862 mL 1.4312 mL 2.8625 mL 7.1561 mL
    15 mM 0.1908 mL 0.9542 mL 1.9083 mL 4.7708 mL
    20 mM 0.1431 mL 0.7156 mL 1.4312 mL 3.5781 mL
    25 mM 0.1145 mL 0.5725 mL 1.1450 mL 2.8625 mL
    30 mM 0.0954 mL 0.4771 mL 0.9542 mL 2.3854 mL
    40 mM 0.0716 mL 0.3578 mL 0.7156 mL 1.7890 mL
    50 mM 0.0572 mL 0.2862 mL 0.5725 mL 1.4312 mL
    60 mM 0.0477 mL 0.2385 mL 0.4771 mL 1.1927 mL
    80 mM 0.0358 mL 0.1789 mL 0.3578 mL 0.8945 mL
    100 mM 0.0286 mL 0.1431 mL 0.2862 mL 0.7156 mL
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    SRPIN340 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SRPIN340218156-96-8SRPK inhibitorSRPIN 340SRPIN-340SRPKVirus ProteaseSerine-arginine protein kinasesInhibitorinhibitorinhibit

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